**3.3 Somatic cells**

This way has the main advantage of being easy to do, which permits to sample tissues quickly and simply on a large number of individuals. But the main difficulty is the recreation of animals after somatic cloning, still not well controlled. The cells can be isolated from different tissues: skin, cartilage, bones, blood… These cells, present in a large number in each organism, would allow the reconstitution of an individual of the same generation than the tissues giver after somatic cloning (Chesne *et al.,* 2002).

More recently, a new opportunity came with induced pluripotent stem cells, which are able to differentiate in many tissues. These cells are potential vectors for genetic traits transmission and could be used after thawing as an important core source to regenerate many individuals after nuclear transfer (Honda *et al*, 2010).
