**1. Introduction**

The purpose of this chapter is to introduce new methods of analysis, to evaluate the final quality of human origin bio therapeutics products generated in Tissue Banks (TB), using well developed and known techniques in various fields of Physics, Chemistry and Biology as applied X – Ray diffraction (XRD), and Raman Scattering (RS).

Cryopreservation techniques are fundamental supports in the conservation procedures of biological materials in TB work. However, controversial views remain on the effects at the molecular level that cryogenic temperatures and thawing could produce on the functional structures of tissues. The same concept can be sustained to glycerolized tissue preservation. Taking into account this scope, we implemented a methodological scheme to analyze tissue specimens before and after programmed cryopreservation, or glycerolization in order to find structural differences in the basic material constitutive collagen, using the techniques formerly mentioned: diffractive and scattering.

It is noteworthy that both methods of analysis can be applied to any type of tissue preserved for the aforesaid purposes, with other conservation techniques, such as freeze drying or un programmed freezing.
