**9. Conclusions**

The aim of our study was the analysis of tissues produced in TB for therapeutic purposes, by known techniques, XRD and RS, able to approach the study of structures at the molecular level, mainly in reference to collagen, the fundamental component of ECM,. Taking account the hypothesis that preservation techniques introduce changes in the matrix elements of the tissues, we subjected amniotic membrane and arterial vascular samples under two types of procedures: a) cryopreservation-defrost as physical process, and glycerolization deglycerolization as chemical process. Regarding to the results of our tests, we accept our hypothesis and concluded that while cryopreservation modifies the structural arrangement of collagen at the level of ECM, glycerolization changes molecular d spacing of biological polymers, besides the aforementioned order. However, the changes between the processed vascular tissues and amniotic membrane are different, because while the vascular cryopreservation increases the molecular order of the crystalline structure measured by OPC value, the amniotic membrane glycerolization and cryopreservation decrease the referred molecular order. These differences are interpreted as the result of complex physicochemical phenomena that occur during preservation procedures on molecular structures and its designs. These phenomena promote variations in the tissue molecular complexity and order distribution. Preliminary data from the Raman tests corroborate the hypothesis of specific modifications in the molecular structures. The consequences of these findings on the allograft biological behavior applied to clinical purposes is a challenge to research and development. Both types of tissues studied are widely applied in the world with beneficial results for the restoration of altered structures and functions in the recipients. But the gold standard allograft is not yet produced, so it is necessary to obtain the allograft that better reproduce the structural and functional conformation of the patient implanted. This objective will be achieved through the best inter relation between recipient structures and the preserved tissues applied, at molecular level to obtain the better possible allograft behavior and patency. Mainly, taking account the advanced development of applied bio engineering, and the design of complex products (composites) that combine different types of cells and artificial, biological, or modified scaffolds.

#### **10. References**


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The aim of our study was the analysis of tissues produced in TB for therapeutic purposes, by known techniques, XRD and RS, able to approach the study of structures at the molecular level, mainly in reference to collagen, the fundamental component of ECM,. Taking account the hypothesis that preservation techniques introduce changes in the matrix elements of the tissues, we subjected amniotic membrane and arterial vascular samples under two types of procedures: a) cryopreservation-defrost as physical process, and glycerolization deglycerolization as chemical process. Regarding to the results of our tests, we accept our hypothesis and concluded that while cryopreservation modifies the structural arrangement of collagen at the level of ECM, glycerolization changes molecular d spacing of biological polymers, besides the aforementioned order. However, the changes between the processed vascular tissues and amniotic membrane are different, because while the vascular cryopreservation increases the molecular order of the crystalline structure measured by OPC value, the amniotic membrane glycerolization and cryopreservation decrease the referred molecular order. These differences are interpreted as the result of complex physicochemical phenomena that occur during preservation procedures on molecular structures and its designs. These phenomena promote variations in the tissue molecular complexity and order distribution. Preliminary data from the Raman tests corroborate the hypothesis of specific modifications in the molecular structures. The consequences of these findings on the allograft biological behavior applied to clinical purposes is a challenge to research and development. Both types of tissues studied are widely applied in the world with beneficial results for the restoration of altered structures and functions in the recipients. But the gold standard allograft is not yet produced, so it is necessary to obtain the allograft that better reproduce the structural and functional conformation of the patient implanted. This objective will be achieved through the best inter relation between recipient structures and the preserved tissues applied, at molecular level to obtain the better possible allograft behavior and patency. Mainly, taking account the advanced development of applied bio engineering, and the design of complex products (composites) that combine different types of cells and artificial, biological, or

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**9. Conclusions** 

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