**2.15 Statistical analysis**

The data collected on the parameters, motility, fertility and hatchability was subjected to standard statistical analysis. The data collected were analyzed using analysis of variance (ANOVA) to find a level of significance at p < 0.05. The number of motile sperm counted per square of hemocytometer and percentage of motile sperm obtained after cryopreservation were subjected to Duncan's multiple range test to evaluate effects of types of cryopreservatives as well as egg clutch weights and sperm dilutions and period of cryopreservation on sperm motility. The data collected on motility, fertility and hatchability in the first trial were subjected to 2- way ANOVA at a significance level of p < 0.05. The bar charts and line chart showing the relationship between the period of refrigeration and the % motility for the various extenders were also employed for better understanding of the results.
