**2.2.3 Calibration, accuracy and precision**

The developed analytical method was validated by evaluating the linear dynamic range, precision, accurate, limit of detection (LOD) and limit of quantification (LOQ) as well as by applying the standard addition technique. Under the optimized experimental conditions, a linear calibration graph was obtained for 0.01-120 mg L-1 iron (II) under the optimum conditions with a regression coefficient of 0.9914. The relative standard deviation for the determination of 5 g L-1 iron (II). was 0.85 % for 10 replicate injections. The limit of detection (blank signal plus three times the standard deviation of the blank) was 0.4 g L-1. The sample throughput of the proposed method was almost 60 sample h-1.
