**9. Acknowledgements**

To the National Council for Scientific and Technological Development (CNPq), process number 479329/2009-5, and Coordination of Improvement Staff (CAPES) for their financial support.

#### **10. References**


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Finally it is worth mentioning that the spectrophotometry should be more standardized and published as an analysis tool to better use of the bees by the beekeepers in beekeeping

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**15** 

*Romania* 

**Multivariate Data Processing in** 

Zoltan Szabadai2, Vicenţiu Vlaia2, Ioan Ţăranu1,

*2University of Medicine and Pharmacy "Victor Babeş",* 

**Chemical Systems** 

**Spectrophotometric Analysis of Complex** 

*1National Institute of Research-Development for Electrochemistry and Condensed Matter,* 

There are a great variety of processing the analytical spectroscopy data, especially useful in multicomponent systems [Ewing et al., 1953; Garrido et al., 2004; Lykkesfeld, 2001; Oka et al., 1991; Sánchez & Kowalski, 1986]. These methods essentially are based on different strategies of mathematical strategies including specific formalism of mathematical statistics and of matrix algebra [Garrido et al., 2004; Szabadai, 2005]. The matrix-based methods reffer to quantitative analysis [Bosch-Reigh et al., 1991; Garrido et al., 2008; Li et al., 2011; Lozano et al., 2009; Ruckenbusch et al., 2006; Szabadai, 2005], to determination of the number of independent chemical equilibria in multicomponent systems [Szabadai, 2005] and for correction the action of various perturbing factors such as stray light or backgroud absorption [Burnius, 1959; Fox & Mueller, 1950; Melnick, 1952; Morton & Stubbs, 1946, 1947,

In the present chapter original approaches of matrix treatment of the aforementioned items are presented, with special consideration to the simultaneous assay of compounds in a mixter, to backgruond correction procedures and to the standard addition method in a

The issue of the quantitative analysis of a mixture, when the components do not interact chemically, can be approached, in a rigorous and general manner, with the help of matrix computation [Ewing et al., 1953; Garrido et al., 2004, 2008; Lozano et al., 2009; Lykkesfeld, 2001; Oka et al., 1991; Ruckenbusch et al., 2006; Sánchez & Kowalski, 1986; Szabadai, 2005]. In the case of a mixture with M component, the quantitative determination of the components, one has to measure the absorbance at distinct values of wavelength ( > M). Given a set of N standard solutions (N > M and supposing that, as a rule, each standard solution may contain all of M chemical components of interest in known concentrations), absorbances are to be measured at the same set of wavelengths and in identical conditions

1948; Owen, 1995; Page & Berkovitz, 1943; Szabadai, 2005].

**2. Simultaneous assay of nonreacting compunds in a mixture** 

**1. Introduction** 

generalized form.

as done for standard solutions.

Bogdan-Ovidiu Ţăranu1, Lavinia Vlaia2 and Iuliana Popa1

