**4. Conclusion**

Spectrophotometric determinations of drugs in Vis-UV region are ones from simpler and cheaper methods used in quantitative pharmaceutical analysis. The key problem in application of these methods in analysis of multicomponents mixture is achievement of desired selectivity. Above-mentioned procedures shown that using various methods of chemical derivatization (significant for analysis in Vis region) or ones based on mathematical extends (significant for analysis in UV region) the selective determination of such labile drug as β-lactam antibiotics was possible. Taking into consideration variability of stability of some β-lactam analogs (in the presence of impurities, degradation products, inhibitors of β-lactamases, excipients and other drugs) and pathways of their degradation, confirmation of selectivity of method and values of other validation parameters for each analogs is required. It is important that analytical method used as alternative, referential one should not use spectrophotometric properties of compound as the base of detection. Recently, the development of chemometric procedures, as the complementary method for spectrophotometric ones, is often supported by suitable, high level software and equipment of apparatus allowing to assume that analysis based on calculated algorithms meet all validation criteria and can replace these methods which require pre-treatment of samples. Moreover, these methods permit multiple determination of the same sample and can be used in routine control analysis of intravenous pharmaceutical dosage forms. Additionally, proposed conditions of determination using spectrophotometric methods are in accordance with all criteria identified as the trends of "*green chemistry*". A enrichment of visible spectrophotometric methods by the application of FIA and SIA permit accept of challenges of modern industrial-scale pharmaceutical analysis [60]. It can be expected that spectrophotometric methods enriched by chemometric procedures will become an important field of pharmaceutical analysis including also labile drugs.
