**2.4 Total polyphenol content**

The whole and clarified acid lime juices were previously lyophilized in a Labconco Lyophilizer (model 75223, Kansas City, Missouri, USA) at - 40 ºC, under vacum (< 200 x 10-3 mBar), for 18 hours.

The Folin-Ciocalteau method (Folin & Ciocalteau, 1927; Singleton & Rossi, 1965) was used for quantification of the total polyphenol in the juices. After reaction with the Folin-Ciocalteau reagent (2N) (Sigma Aldrich, Steinhem, Germany), absorbance was measured at 760 nm (λmax = 765 nm) using a UV- vis spectrophotometer (Shimadzu, model 1240, Kyoto, Japan). The amount of polyphenols was calculated from a standard curve of gallic acid (MP Biomedicals, Ilkirch, France). The results were expressed as mg of gallic acid equivalents (GAE)/mL of juice sample (Andrade et al., 2007).

#### **2.5 Statistical analysis**

Statistical analysis was performed using one-way ANOVA and mean values were compared applying Duncan's multiple range tests using Minitab Microsoft Excel. Trends were considered significant when means of compared sets differed at *P* < 0.05.
