**2.3.2 Quantitative 31P NMR analysis**

Quantitative 31P NMR analysis was carried out in a Bruker Avance 600 MHz automatic spectrometer using triphenyl phosphate as internal standard (Spectral Service GmbH, Köln, Germany) (Diehl, 1997; 2001; 2008). Phospholipid content of samples obtained under different conditions of enzymatic hydrolysis, was determined by this spectroscopic technique.
