**2.1 Instruments**

Gas chromatograph/mass spectrometer (GC/MS) was a Hewlett Packard HP-5890 series and a JEOL auto mass system. Total nitrogen (T-N) analysis was performed using a Tokyo Kasei Ind. Co. TCI-NOX 1000, GASTORR GT-102, VISIBLE DETECTOR S-3250 and AUTO SAMPLER SS-3600, while other determinations Hitachi Co. U-2000A a spectrophotometer was used.
