**2.6 Particle size measurements**

Particle size distribution, and De Brouckere (D[4,3]) and Sauter (D[3,2]) mean diameters of particles of the emulsions were determined with a particle size analyzer (Malvern Mastersizer 2000E, Malvern Instruments Ltd., Worcestershire,U.K.). Samples were diluted in the water bath of the dispersion system (Hydro 2000MU), which is a laser diffraction based particle size analyzer (Márquez & Wagner, 2010). This determination was carried out in triplicate for each case.

#### **2.7 Statistical analysis**

Data were evaluated by analysis of variance (ANOVA) using the software Systat® 12.0 (Systat, 2007). For this purpose, differences were considered significant at *p* < 0.05.

Emulsifying Properties of Hydrolized

Sunflower Lecithins by Phospholipases A2 of Different Sources 43

a Mean values are shown (*n* = 3). The coefficient of variation was lower than 5%

(a) DSL, 0.1%; (b) SHLP, 0.1%

Fig. 1. Backscattering (%BS) profiles of O/W emulsions (30:70 wt/wt) with the addition of:


Table 1. Phospholipid (PL) composition of modified sunflower lecithins by 31PNMRa
