**1. Introduction**

296 Soybean – Genetics and Novel Techniques for Yield Enhancement

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Revolution in plant biotechnology can be categorized into in vitro culture and genetic transformation. Plant regeneration was successfully achieved in 1950's while production of transgenic plant was accomplished in 80's. For production of transformants, in vitro culturing strategies are prerequisite.

Soybean has been cultured through organogenesis and embryogenesis but still it is considered recalcitrant. Many explant types has been subjected for shoot induction but immature cotyledons and cotyledonary node of mature seeds got attention in recent years due to high number of shoot production in less time period. But still nature of culture media, application of plant growth regulator and environmental conditions affect on regeneration efficiency. If all constrains are consistent, genotype dependency along with age of explants can not be neglected.
