**4.2.2 Production of salt-tolerant acid protease by** *B. megaterium* **KLP-98**

Proteolytic bacteria from the fermented squid were isolated by BHI agar. After inoculating 0.1 mL of fluid of the squid with 10% NaCl concentration fermented at room temperature for 2 months, the culture media were incubated at 37 °C for 48 h. The highest transparent colony on the culture medium was isolated, identified and inoculated in BHI broth media with 10% NaCl. 500 mL of medium in a 1 L wide-mouth culture flask was inoculated with 10 mL suspension prepared from BHI broth media cultivated at 37 °C for 72 h. The culture was incubated at 37 °C for 5 days in a shaking incubator at 150 rpm. The optimal protease production reached 520 U/L (Hwang et al., 2010).
