**4.5.3 Effects of temperature on enzyme activity and stability**

318 Soybean – Genetics and Novel Techniques for Yield Enhancement

A

B

C Fig. 2. SDS-PAGE electrophoresis of proteases produced by *B. subtilis* JM3, *B. megaterium*

KLP-98 and *A. oryzae* LK-101.

As shown in Table 7, the optimal temperature for the activity of protease produced by *A. oryzae* LK-101 was lower than those of the other two proteases, while the stability of proteases produced by *A.* LK-101 and *B. megaterium* KLP-98 was better than that of the protease produced by *B. subtilis* JM3 (W.J. Kim and S.M. Kim, 2005; Fu et al., 2008; Hwang et al., 2010).


Table 7. Effects of temperature on activity and stability of purified salt-tolerant acid proteases.
