**5.2.3 The number of retained leaves of soybean scion**

The retained leaves numbers of scion when grafting may also have an important impact on the mutation rate. In general, the retained leaves numbers of scion negatively correlate with the mutation rate of grafted progeny. According to our experience, retaining 1-2 leaves of soybean scion is the best option for the early 30 days after grafting.
