**2.4 Genetic linkage analysis**

Primers that distinguished the bulks and the parents were tested on the entire F2 population. Marker (RAPD, AFLP, and SSR) data obtained from 94 F2 progenies of population 1 and 2 were used to construct genetic linkage map including *Ti* locus using the computer program MAPMAKER v. 3.0 (Lander et al., 1987). Markers were assigned to group using the "Group" command, with a LOD score of 4.0 and maximum recombination distance of 50 cM. Once markers were assigned to a given linkage group, the most linkage marker order within the group was determined using the "Compare" command. Marker orders within each linkage group were ascertained by use of "Ripple" command. Map distance (cM) were computed using the Kosambi (Kosambi, 1944) mapping function.
