**2.8 Transformation of vegetable oils to other supramolecular structures**

In addition to the above described wide numbers of supramolecular structures, we also received rubber like, membrane/sheet like and microemulsion structures from vegetable oil under insitu conditions. The following figure depicts the images of rubber like (a), membrane/sheet like (b) and microemulsion structures (c).

Fig. 8. Different supramolecular structures from vegetable oil (a) rubber like; (b) membrane/sheet like; (c) microemulsion structures.

The major differences in the above said three structures are lies with the nature of the vegetable oil chosen. The rubber like material is obtained when the oil is heated near boiling point and then cooled and then incorporated in the mineral medium. The important message obtained from this material preparation is, the whole process is proceeded with out any microbes. Hence, self-assembly would have been takes place by mere self-assembly of the saturated fatty acids.

With reference to membrane/sheet like material and microemulsion structures, marine *Bacillus* sps is involved in the presence of mineral salts.

All these supramolecular structures received from vegetable oils under *in situ* condition during the growth of organisms pose a serious question on whether application of all the microbial products separately without microbes will provide the same kind of materials or not. Thus experiments were conducted separately with the selected oils with the enzyme lipase obtained from Sigma and the biosurfactants separated from the species used in the present study, mineral medium and the environmental conditions like agitation at 180-200 rpm. We received supramolecular structures of vesicles, multilamellar vesicles and selfhealing materials. Other said structures could not be received and implies they will be formed only in the presence of microbes.

The following schematic representation revealed the formation of various supramolecular structures from vegetable oils and the transformation phases observed in the presence and the absence of microbes.

In addition to the above described wide numbers of supramolecular structures, we also received rubber like, membrane/sheet like and microemulsion structures from vegetable oil under insitu conditions. The following figure depicts the images of rubber like (a),

**Rubber like Membrane/sheet like Microemulsion**

**2.8 Transformation of vegetable oils to other supramolecular structures** 

membrane/sheet like (b) and microemulsion structures (c).

**a b c**

Fig. 8. Different supramolecular structures from vegetable oil (a) rubber like;

The major differences in the above said three structures are lies with the nature of the vegetable oil chosen. The rubber like material is obtained when the oil is heated near boiling point and then cooled and then incorporated in the mineral medium. The important message obtained from this material preparation is, the whole process is proceeded with out any microbes. Hence, self-assembly would have been takes place by mere self-assembly of

With reference to membrane/sheet like material and microemulsion structures, marine

All these supramolecular structures received from vegetable oils under *in situ* condition during the growth of organisms pose a serious question on whether application of all the microbial products separately without microbes will provide the same kind of materials or not. Thus experiments were conducted separately with the selected oils with the enzyme lipase obtained from Sigma and the biosurfactants separated from the species used in the present study, mineral medium and the environmental conditions like agitation at 180-200 rpm. We received supramolecular structures of vesicles, multilamellar vesicles and selfhealing materials. Other said structures could not be received and implies they will be

The following schematic representation revealed the formation of various supramolecular structures from vegetable oils and the transformation phases observed in the presence and

(b) membrane/sheet like; (c) microemulsion structures.

*Bacillus* sps is involved in the presence of mineral salts.

formed only in the presence of microbes.

the saturated fatty acids.

the absence of microbes.

Fig. 9. Schematic representation of different self-assembled supramoelcular structures using plant triglycerides with microbes, with microbial products and by simple physical treatments.

Transformation of Soybean Oil to Various Self-Assembled Supramolecular Structures 295

Isbell, TA. (1994). Acid catalyzed condensation of oleic acid into estolides and polyestolides.

Jiang, G. Wang, L. Yu, H. Chen, C. Dong, X. Chen, T. Yang, Q. (2006). Macroscopic selfassembly of hyperbranched polyesters. *Polymer*, Vol.47, pp. (12–17). Kestelman, V. Veslovsky, R. (2001). *Adhesion of polymer*, Kindle Edition, McGraw –Hill

Lehn, J.(2005).Supramolecular polymer chemistry- Scope and perspectives. In.

Lei, G. MacDonald, RC. (2003). Lipid Bilayer Vesicle Fusion: Intermediates Captured by

Liu, S. Volkmer, D. Kurth, DG. (2003). Functional polyoxometalate thin films via

Monnard, PA. (2005). Catalysis in abiotic structured media: an approach to selective synthesis of biopolymers. *Cell Mol Life Science*, Vol. 62, No.5, pp.(520–534). Montarnal, D. Cordier, P. Ziakovic, CS. Tournilhac, F. Leibler, L. (2008). Synthesis of self-

Novales, B. Navailles, L. Axelos, M. Nallet, F. Douliez, JP. (2008). Self-assembly of fatty acids

Ochiai, M. Miyamoto, K. Suefuji, K. Shiro, M. Sakamoto, S. Yamaguchi K. (2003). Synthesis

Rogers, A. Smith, AK. Wright, AJ. Marangoni, AJ. (2007). Novel cryo-SEM technique for

Singh, K. Marangoni, DJ. Quinn, JG. Singer, RD. (2009). Spontaneous vesicle formation with

Sowerby, SJ. Cohn, CA. Heckl, WM. Holm, NG. (2001). Differential adsorption of nucleic

Sowerby, SJ. Petersen, GB. Holm, NG.(2002). Primordial coding of amino acids by adsorbed

Lsraelachvili, J. (1985). *Intermolecular and surface forces* (New York: Academic)

and hydroxyl derivative salts. *Langmuir*, Vol. 24,pp.(62-68).

Scheludko, A. (1968). Thin liquid films. *Adv Coll Inter Science*, Vol.1, pp. (391-464).

purine bases. *Orig Life Evol Biosphere*,Vol. 32, No. 1. pp.(35– 46).

Lyons, JP. (1969). *Power steering process and lubricating composition*. US Patent 3,429,820 Markevich, MA. Rytov, BL. Vladimirov, LV. Shashkin, DP. Shiryaev, PA. Solovev, AG.

Supramolecular polymers ed.Alberto Ciferri, CRC Press, Taylor and Francis,

High-Speed Microfluorescence Spectroscopy. *Biophysical Journal*, Vol.85, No. 3,

electrostatic layer-by-layer self-assembly. *J Cluster Science*, Vol. 14, No. 3, pp. (405-

(1986). Structural organization in epoxy polymers and oligomers. *Vysokomolek* 

healing supramolecular rubbers from fatty acid derivatives, diethylene triamine, and urea. *Journal of Polymer Science*: *Part A: Polymer Chemistry*, Vol. 46, pp.(7925–

and structure of supramolecular complexes between 1-alkynyl (phenyl) (tetrafluoroborato)-l3-iodanes and 18-crown-6. *Tetrahedron*, Vol. 59, pp.(10153–

imaging vegetable oil based organogels. *J Am Oil Chem Society*, Vol. 84, pp. (899–

an ionic liquid amphiphile. *Journal of Colloids and Interface Science*, Vol.335, pp. (105–

acid bases: relevance to the origin of life. *Proc Natl Acad Sci USA*, Vol. 98, No.3, pp.

*J Am Oil Chem Soc*, Vol.71, pp. (169-174).

*Soedin A*, Vol. 28 No.8, pp.( 1595–1602).

publishers, pp. 22-97.

FL,USA pp.1-27.

pp.(1585–1599).

419).

7936).

10158).

906).

111).

(820–822).
