**2.2 Phenotypic characterization**

Each fungus was inoculated with a single touch in the center of a Petri dish containing PDA and incubated under the conditions previously described. Macro and microscopic observations were conducted after 15 days, taking into account color and diameter of the colony, red pigment production (cercosporin) and the presence or absence of typical elements of reproduction. For the latter case, plates were discarded only after 25 days of incubation.

Cercosporin production was confirmed following Jenns et al. methodology (Jenns et al, 1989) with slight modifications as described by González et al. (González et al., 2008).

Phenotypic and Genotypic Variability in

analyzed by Ward method.

*Cercospora kikuchii* Isolates from Santa Fe Province, Argentina 101

AGNES makes agglomerative hierarchical clusters, the distance between clusters (k) being

Silhouette Coefficient (SC) and Agglomeration Coefficient (AC) were used for FANNY and

OPA-01 5'-CAGGCCCTTC-3' OPA-02 5'-TGCCGAGCTG-3' OPA-03 5'-AGTCAGCCAC-3' OPA-04 5'-AATCGGGCTG-3' OPA-05 5'-AGGGGTCTTG-3' OPA-06 5'-GGTCCCTGAC-3' OPA-07 5'-GAAACGGGTG-3' OPA-08 5'-GTGACGTAGG-3' OPA-09 5'-GGGTAACGCC-3' OPA-10 5'-GTGATCGCAG-3' OPA-11 5'-CAATCGCCGT-3' OPA-12 5'-TCGGCGATAG-3' OPA-13 5'-CAGCACCCAC-3' OPA-14 5'-TCTGTGCTGG-3' OPA-15 5'-TTCCGAACCC-3' OPA-16 5'-AGCCAGCGAA-3' OPA-17 5'-GACCGCTTGT-3' OPA-18 5'-AGGTGACCGT-3' OPA-19 5'-CAAACGTCGG-3' OPA-20 5'-GTTGCGATCC-3'

**Fungi j Total** 

**1 0** 

**Total** a+c b+d a+b+c+d

Table 4. Contingency table summarizing the coincidences in the presence/absence of bands between pairs of fungi. Letters i and j: correspond to the same fungus or to different fungi; 1:

presence of bands; 0: absence of bands; a, b, c and d are the frequencies1.

. No 0-0 matches in numerator or denominator.

**1** a b a+b **0** c d c+d

AGNES, respectively, to evaluate cluster quality (Kaufman & Rousseeuw, 1990).

**Oligonucleotide Sequence** 

Data were processed with R version 2.10.1 Software (Torgo, 2003).

Table 3. Oligonucleotides used in RAPD reaction

**Fungi i** 

coefficient of distance is given by:

 1

> *b c <sup>J</sup> abc*
