**8. Surgical treatment**

#### **8.1 Principles of valve reconstruction**

Guided by functional valve analysis, the surgeon should indentify all the lesions. The ultimate aim of valve reconstruction is to restore durable normal valve function. Three basic conditions are required to achieve this goal: preserving or restoring normal leaflet motion of all valve segments, creating a large surface of leaflet coaptation, remodeling the mitral valve orifice. Ideally correct application of repair techniques will produce appropriate coaptation. The rough zones of the leaflet should be in contact. The ratio of leaflet surface area to valve orifice area should be corrected to its natural 2:1 ratio. Because annular dilatation is a component of chronic mitral regurgitation, some type of annular support is necessary. Annuloplasty often completes the repair but cannot compensate for an inadequate repair. Echocardiography with color flow and spectral Doppler evaluation is an important noninvasive method for assessing the significance of cardiac murmurs. Information regarding valve morphology and function, chamber size, wall thickness, ventricular function, pulmonary and hepatic vein flow, and estimates of pulmonary artery pressures can be readily integrated. Zoghbi et al., 2003 presented qualitative and quantitative parameters useful in grading mitral regurgitation severity (Table#5).


#### Table 5. Qualitative and quantitative parameters useful in grading mitral regurgitation severity.

Three different MV operations are currently used for correction of MR: 1) MV repair; 2) MV replacement with preservation of part or all of the mitral apparatus; and 3) MV replacement with removal of the mitral apparatus. Each procedure has its advantages and disadvantages, and therefore, the indications for each procedure are somewhat different (Bonow et al., 2008).

In most cases, MV repair is the operation of choice when the valve is suitable for repair and appropriate surgical skill and expertise are available. This procedure preserves the patient's native valve without a prosthesis and therefore avoids the risk of chronic anticoagulation (except in patients in atrial fibrillation) or prosthetic valve failure late after surgery. Additionally, preservation of the mitral apparatus leads to better postoperative LV function and survival than in cases in which the apparatus is disrupted. Improved postoperative function occurs with repair because the mitral apparatus is an integral part of the left ventricle that is essential for maintenance of normal shape, volume, and function of the left ventricle. However, MV repair is technically more demanding than MV replacement, may require longer extracorporeal circulation time, and may occasionally fail. In USA (Savage et al., 2003) and Europe (Lung et al., 2003) the valve with MV regurgitation is repaired in only 50% of cases. Valve morphology and surgical expertise are of critical importance for the success of valve repair. The reoperation rate after MV repair is similar to the reoperation rate after MV replacement. There is a 7% to 10% reoperation rate at 10 years in patients undergoing MV repair, usually for severe recurrent MR. Approximately 70% of the recurrent MR is thought to be due to the initial procedure and 30% to progressive valve disease. The reoperation rate is lower in those patients who had the initial operation for posterior leaflet abnormalities than in those who had bileaflet or anterior leaflet abnormalities. In many cases, the type of operation, MV repair versus replacement, is important in timing surgery. In fact, although the type of surgery to be performed is never actually established until the operation, many situations lend themselves to preoperative prediction of the operation that can be performed. This prediction is based on the skill and experience of the surgeon in performing repair and on the location and type of MV disease that caused the MR. Nonrheumatic posterior leaflet prolapse due to degenerative MV disease or a ruptured chordae tendineae can usually be repaired using a resection of the portion of the valve and an annuloplasty. Involvement of the anterior leaflet or both anterior and posterior leaflets diminishes the likelihood of repair because the operation requires other interventions, such as chordal shortening, chordal transfer, and innovative anatomic repairs. Consequently, the skill and experience of the surgeon are probably the most important determinants of the eventual operation that will be performed.

#### **8.2 Surgical approach**

10 Front Lines of Thoracic Surgery

Guided by functional valve analysis, the surgeon should indentify all the lesions. The ultimate aim of valve reconstruction is to restore durable normal valve function. Three basic conditions are required to achieve this goal: preserving or restoring normal leaflet motion of all valve segments, creating a large surface of leaflet coaptation, remodeling the mitral valve orifice. Ideally correct application of repair techniques will produce appropriate coaptation. The rough zones of the leaflet should be in contact. The ratio of leaflet surface area to valve orifice area should be corrected to its natural 2:1 ratio. Because annular dilatation is a component of chronic mitral regurgitation, some type of annular support is necessary. Annuloplasty often completes the repair but cannot compensate for an inadequate repair. Echocardiography with color flow and spectral Doppler evaluation is an important noninvasive method for assessing the significance of cardiac murmurs. Information regarding valve morphology and function, chamber size, wall thickness, ventricular function, pulmonary and hepatic vein flow, and estimates of pulmonary artery pressures can be readily integrated. Zoghbi et al., 2003 presented qualitative and quantitative

parameters useful in grading mitral regurgitation severity (Table#5).

Table 5. Qualitative and quantitative parameters useful in grading mitral regurgitation

Three different MV operations are currently used for correction of MR: 1) MV repair; 2) MV replacement with preservation of part or all of the mitral apparatus; and 3) MV replacement

**8. Surgical treatment** 

severity.

**8.1 Principles of valve reconstruction** 

According to M. Scorsin (Scorsin et al., 2010), in 90% cases of posterior mitral valve prolapse is repaired with standart techniques: 1 - quadrangular \ triangular resection + placation of annulus, sliding, folding; 2- artificial chordate sutures. Current techniques for anterior leaflet prolapsed include valvular and subvalvular approach. In turn, valvular approach include papillary muscle placation, chordal shortening, leaflet resection, transposition of chordate. Subvalvular approach include artificial chordate. Among several repair techniques chordal replacement using ePTFE sutures has been introduced into clinical practise by Frater. There are several advantages to use of ePTFE suture for chordal replacement preservation of native valve anatomy, physiological leaflet motion and creation of large

Mitral Valve Subvalvular Apparatus Repair with Artificial Neochords Application 13

Chordae tendinae reconstruction, in patients with prolapsed of anterior leaflet was done by **Matsumoto et al., 1999** in children using the following technique: double-armed mattress e-PTFE sutures were passed through the free prolapsed edge from the ventricular side to the atrial side and then the two ends were passed through the papillary muscle at 3 to 4 mm from its top, drawing the free edge down to the entry point on the papillary muscle of the two ends of the suture. The sutures were passed through a pledget, which would be on the side where the sutures emerged from the papillary muscle. The knot was tied at the level of the opposing normal leaflet. The new chorda was pulled back through the papillary muscle until the pledget came up against the muscle. Another e-PTFE suture was placed in the same fashion. A Kay-Reed annuloplasty was

**Kawahira et al., 1999** used 4-0 e-PTFE sutures through the prolapsed leaflet from its ventricular to atrial aspect, placing pledgets for reinforcement on the ventricular surface of the leaflet. The sutures were anchored to the papillary muscles in a mattress fashion. This maneuver could be carried out in reverse order: attaching e- PTFE suture initially to the papillary muscle, subsequently passing it through the leaflet from it's ventricular to atrial aspect. In this circumstance, the knot would be placed on the atrial aspect of the mitral valve. **Adams et al., 2001** placed one or more 4-0 Gore- Tex sutures into the head of the papillary muscle. Papillary muscle exposure was enhanced after quadrangular posterior leaflet resection. Before annuloplasty poor leaflet apposition is present in all leaflet segments with saline testing and segmental anterior leaflet prolapse is best identified by height comparison with a normal reference point. After ring annuloplasty symmetric leaflet apposition limits leaflet incompetence to the prolapsing anterior leaflet segment. Both arms of the previously placed Gore-Tex suture are passed through the margin of the prolapsing leaflet segment. Passing the suture through the free edge of the cusp twice as well as starting with a surgeon's knot are techniques to prevent overaggressive sliding of the knots when

added (Figure 6).

Fig. 6.

tying the Gore-Tex suture (Figure 7).

mitral orifice area. Properly inserted, ePTFE sutures restore stress on the valve leaflet to a normal range. Long-term studies in experimental animals and in humans show that ePTFE chordae do not shrink or stiffen; indeed, these artificial chordae become covered by a fibrosis and intima simulating true chordae.

#### **8.3 Evolution of modern valve repair techniques with artificial chordae**

Medical literature describes numerous techniques to create and to determine ideal length for artificial chordae. Bizzarri et al has analysed evolution of modern valve repair techniques with artificial chordae (Bizzarri et al., 2010). **Morita et al., 1996** were the first to use 4-0 PTFE figure of 8 to repair both leaflets prolapse passing from the papillary body to the leaflet and back adding a Kay annuloplasty at the end of the procedure. **Zussa, 1997**, one of the pioneers of this technique, repaired an anterior leaflet with PTFE strings passing through the head of the papillary muscle and tying over a reinforcing autologous pericardial pledget. The strings were then anchored to the free margin of the anterior mitral leaflet at the unsupported areas and reinforced with a small autologous pericardial pledget. The two strands were tied after filling the ventricular cavity with saline solution for adjusting the chordal length. **Murakami et al., 1998** approached the anterior leaflet prolapse using mattress e-PTFE suture with Teflon or autologous pledget passed through the free margin of the leaflet from the ventricular side to the atrial side. The two arms of the suture, reinforced with pledgets, were brought down to the papillary muscle and passed through it. The length of the e-PTFE chordae was then adjusted by approximating the coapting area of the opposite leaflet and the ends of the sutures were then tied together (Figure 5).

Fig. 5.

Chordae tendinae reconstruction, in patients with prolapsed of anterior leaflet was done by **Matsumoto et al., 1999** in children using the following technique: double-armed mattress e-PTFE sutures were passed through the free prolapsed edge from the ventricular side to the atrial side and then the two ends were passed through the papillary muscle at 3 to 4 mm from its top, drawing the free edge down to the entry point on the papillary muscle of the two ends of the suture. The sutures were passed through a pledget, which would be on the side where the sutures emerged from the papillary muscle. The knot was tied at the level of the opposing normal leaflet. The new chorda was pulled back through the papillary muscle until the pledget came up against the muscle. Another e-PTFE suture was placed in the same fashion. A Kay-Reed annuloplasty was added (Figure 6).

Fig. 6.

12 Front Lines of Thoracic Surgery

mitral orifice area. Properly inserted, ePTFE sutures restore stress on the valve leaflet to a normal range. Long-term studies in experimental animals and in humans show that ePTFE chordae do not shrink or stiffen; indeed, these artificial chordae become covered by a

Medical literature describes numerous techniques to create and to determine ideal length for artificial chordae. Bizzarri et al has analysed evolution of modern valve repair techniques with artificial chordae (Bizzarri et al., 2010). **Morita et al., 1996** were the first to use 4-0 PTFE figure of 8 to repair both leaflets prolapse passing from the papillary body to the leaflet and back adding a Kay annuloplasty at the end of the procedure. **Zussa, 1997**, one of the pioneers of this technique, repaired an anterior leaflet with PTFE strings passing through the head of the papillary muscle and tying over a reinforcing autologous pericardial pledget. The strings were then anchored to the free margin of the anterior mitral leaflet at the unsupported areas and reinforced with a small autologous pericardial pledget. The two strands were tied after filling the ventricular cavity with saline solution for adjusting the chordal length. **Murakami et al., 1998** approached the anterior leaflet prolapse using mattress e-PTFE suture with Teflon or autologous pledget passed through the free margin of the leaflet from the ventricular side to the atrial side. The two arms of the suture, reinforced with pledgets, were brought down to the papillary muscle and passed through it. The length of the e-PTFE chordae was then adjusted by approximating the coapting area of the opposite leaflet and the ends of the sutures were then tied

**8.3 Evolution of modern valve repair techniques with artificial chordae** 

fibrosis and intima simulating true chordae.

together (Figure 5).

Fig. 5.

**Kawahira et al., 1999** used 4-0 e-PTFE sutures through the prolapsed leaflet from its ventricular to atrial aspect, placing pledgets for reinforcement on the ventricular surface of the leaflet. The sutures were anchored to the papillary muscles in a mattress fashion. This maneuver could be carried out in reverse order: attaching e- PTFE suture initially to the papillary muscle, subsequently passing it through the leaflet from it's ventricular to atrial aspect. In this circumstance, the knot would be placed on the atrial aspect of the mitral valve. **Adams et al., 2001** placed one or more 4-0 Gore- Tex sutures into the head of the papillary muscle. Papillary muscle exposure was enhanced after quadrangular posterior leaflet resection. Before annuloplasty poor leaflet apposition is present in all leaflet segments with saline testing and segmental anterior leaflet prolapse is best identified by height comparison with a normal reference point. After ring annuloplasty symmetric leaflet apposition limits leaflet incompetence to the prolapsing anterior leaflet segment. Both arms of the previously placed Gore-Tex suture are passed through the margin of the prolapsing leaflet segment. Passing the suture through the free edge of the cusp twice as well as starting with a surgeon's knot are techniques to prevent overaggressive sliding of the knots when tying the Gore-Tex suture (Figure 7).

Mitral Valve Subvalvular Apparatus Repair with Artificial Neochords Application 15

**Soga, 2003** made a resection of both the anterior and posterior mitral leaflets and subvalvular apparatus and placed two 3-0 e-PTFE mattress sutures: one placed and tied at the tip of the anterior papillary muscle, and one at the tip of the posterior papillary muscle. The suture of the anterior PM is placed at the 9-10 o'clock position on the mitral annulus (as defined by mid-anterior annulus to be 0 o'clock), and the suture for the posterior PM at the 5-6 o'clock. According to the authors, the length of the artificial CT can be determined during intraoperative cardiac arrest, and may be suitable if the sutures are tied just less than taut before insertion of the prosthetic. After the valve replacement, the motion of the prosthetic leaflets is examined to ensure that the leaf let are not entrapped by the 3-0 e-PTFE

Fig. 8.

Fig. 9.

sutures (Figure 9).

#### Fig. 7.

**Tomita, 2002** applied the method of David to use the reconstruction of the valve with CV-4 e-PTFE sutures. The double armed suture is passed twice through the fibrous portion of the papillary muscle head that anchors the elongated or ruptured chordae and is tied down (seven or eight knots are needed for this type of suture material). The two arms of the suture are then brought up to the free margin of the leaflet and passed through the point where the original chorda was attached (thickened portion of the leaflet). The needle is brought from the ventricular side of the leaflet to its atrial side and then passed once through the leaflet. The length of the PTFE chordae is adjusted by approving the coating area of the opposite leaflet. Then both ends of the suture are passed through the leaflet again and tied together on the ventricular side. Another PTFE suture is placed when the prolapsed portion is wide. Kay's annuloplasty is added at the end.

With time, it appeared mandatory to find the correct technique to determine the length of the artificial chordae. **Sarsam et al., 2002** passed one or more 5-0 e-PTFE sutures, supported by a felt pledget through the fibrous portion of the papillary muscle. The suture was left untied. The two arms of the suture were then passed once through the rough free edge of the prolapsing leaflet from the ventricular to the atrial side. If the native chorda to the corresponding part of the opposing leaflet are normal, the edges of the anterior and posterior leaflet are temporarily approximated by a simple or figure 8 suture and then the suture is tied against the temporary suture. Three knots are used. The suture in passed again through the edge of the leaflet from the ventricular to the atrial side and tied permanently. The temporary suture is then removed (Figure 8).

#### Fig. 8.

14 Front Lines of Thoracic Surgery

**Tomita, 2002** applied the method of David to use the reconstruction of the valve with CV-4 e-PTFE sutures. The double armed suture is passed twice through the fibrous portion of the papillary muscle head that anchors the elongated or ruptured chordae and is tied down (seven or eight knots are needed for this type of suture material). The two arms of the suture are then brought up to the free margin of the leaflet and passed through the point where the original chorda was attached (thickened portion of the leaflet). The needle is brought from the ventricular side of the leaflet to its atrial side and then passed once through the leaflet. The length of the PTFE chordae is adjusted by approving the coating area of the opposite leaflet. Then both ends of the suture are passed through the leaflet again and tied together on the ventricular side. Another PTFE suture is placed when the prolapsed portion is wide.

With time, it appeared mandatory to find the correct technique to determine the length of the artificial chordae. **Sarsam et al., 2002** passed one or more 5-0 e-PTFE sutures, supported by a felt pledget through the fibrous portion of the papillary muscle. The suture was left untied. The two arms of the suture were then passed once through the rough free edge of the prolapsing leaflet from the ventricular to the atrial side. If the native chorda to the corresponding part of the opposing leaflet are normal, the edges of the anterior and posterior leaflet are temporarily approximated by a simple or figure 8 suture and then the suture is tied against the temporary suture. Three knots are used. The suture in passed again through the edge of the leaflet from the ventricular to the atrial side and tied permanently.

Fig. 7.

Kay's annuloplasty is added at the end.

The temporary suture is then removed (Figure 8).

**Soga, 2003** made a resection of both the anterior and posterior mitral leaflets and subvalvular apparatus and placed two 3-0 e-PTFE mattress sutures: one placed and tied at the tip of the anterior papillary muscle, and one at the tip of the posterior papillary muscle. The suture of the anterior PM is placed at the 9-10 o'clock position on the mitral annulus (as defined by mid-anterior annulus to be 0 o'clock), and the suture for the posterior PM at the 5-6 o'clock. According to the authors, the length of the artificial CT can be determined during intraoperative cardiac arrest, and may be suitable if the sutures are tied just less than taut before insertion of the prosthetic. After the valve replacement, the motion of the prosthetic leaflets is examined to ensure that the leaf let are not entrapped by the 3-0 e-PTFE sutures (Figure 9).

Mitral Valve Subvalvular Apparatus Repair with Artificial Neochords Application 17

**Prêtre et al., 2006** applied the artificial chordae to the mitral valve using an approach through the aortic valve for an anterior and posterior leaflet prolaps. In the anterior repair an atriotomy was performed first, the artificial chordae was placed in the usual manner, and then a flexible annular ring was tied on the mitral annulus. An aortotomy was performed to expose the native chordae and to calibrate the length of the artificial chordae that were locked but not tied down. The mitral valve was inspected through the atriotomy while saline water was injected through the aortotomy in the left ventricle. The chordae were tied from the aortotomy and the incisions closed in the usual fashion. In the posterior leaflet prolapse, repair was done and a ring was inserted using a classical atrial incision. The ascending aorta was opened and the artificial chordae were set on the papillary muscles and the anterior leaflet was calibrated. The valve was re-inspected through the atriotomy with instillation of saline in the left ventricle for adjusting the chordae until they were definitively

**Lawrie et al., 2006** published their experience on 152 consecutive patients. 5-0 PTFE sutures were placed into the bases of the papillary muscles in a figure-8 fashion, and were brought through the free edge of the prolapsing segment. Dots were made to mark the desired final line of leaflet apposition. The left ventricle was inflated with saline solution and the chordal length was adjusted to align the edges of the leaflets. Leaflet alignment was checked and the PTFE was tied down. The knot was locked with a 6-0 polypropylene stitch which was tied over the end of the PTFE to prevent sliding of the PTFE knots. An annuloplasty ring was

**Calafiore, 2006** in the anterior leaflet prolapse passed 4-0 PTFE sutures through the fibrous tip of the papillary muscle and fixed the sutures. The new chorda was passed in the border of the anterior leaflet in the proper place and its final length was measured with a ruler. A mark was applied to indicate this distance and the suture was tied with the aid of a nerve

secured Figure 11.

Fig. 11.

then implanted.

hook (Figure 12).

**Tomita, 2005** repaired chordae tendinae with CV-4 e-PTFE sutures. Double armed sutures are passed twice through the fibrous portion of the PM head that anchors the elongated or ruptured chordae and are tied down (7 or 8 knots are needed for this suture material). The two arms of the suture are brought up to the free margin of the leaflet and passed through the point where the original chorda was attached (thickened portion of the leaflet). The needle is brought from the ventricular side of the leaflet to its atrial side and passed once more through the leaflet. The length of PTFE chordae is adjusted by referring the contact area of the opposite leaflet and then both ends of the suture are passed through the leaflet again and tied together on the ventricular side. When the prolapsed portion became wide, another PTFE suture was placed in the same fashion. At the end Kay's suture annuloplasty (n = 24) or ring annuloplasty was performed. **Minami, 2005** used double armed mattress sutures of 4-0, 5- 0 or 6-0 e-PTFE placed to reinforce with felt pledgets between the PM and free margin of the anterior leaflet. The length of the PTFE sutures was adjusted with the adjacent normal anterior leaflet or facing posterior leaflet. When the prolapsed portion became wide, another suture was placed in the same fashion. The number of sutures ranged from 1 to 3. In addition, Kay annuloplasty was perfomed.

**Matsui, 2005** employed a new device (Matsuda Ika-Kogyo, Tokyo, Japan) consisting of two metallic tubes with a circular, hook shaped distal tip made entirely of stainless steel. The distal tip, which is perpendicularly attached to the inner tube, was designed to hold the Gore-Tex thread at the reference point on the PM immovable. The outer tube could slide on the surface of the inner tube to measure the length from the tip of inner tube to the hook of outer tube. A 4-0 or 5-0 Gore-Tex mattress suture, reinforced with a felt pledget, was placed into the head of the PM. Both arms of the suture where left untied. Length was determined by measuring the distance between the leaflet edge and the site of implantation of the artificial chordae on the PM, using a normal valve segment adjacent to the prolapsing segment as a reference. The distal tip of the inner tube of the device was placed at the sutured site of the artificial chordae on the PM. The proximal hook of the outer tube was slid to the edge of the adjacent non prolapsing leaflet and then fixed at that point after reading the distance between the distal tip and proximal hook to the device. Devices were then moved to the prolapsed segments so as to hold an edge of the prolapsed leaflet with a proximal hook. As the determined distance and edge of the leaflet were fixed with the device, the Gore-Tex suture could be tied in the usual manner without knot slipping. The action of knot-tying itself works to immobilize the device by its strength. After removing the device, followed by saline testing, a Carpenter- Edwards annuloplasty ring was attached according to the size of the mitral annulus (Figure 10).

Fig. 10.

**Prêtre et al., 2006** applied the artificial chordae to the mitral valve using an approach through the aortic valve for an anterior and posterior leaflet prolaps. In the anterior repair an atriotomy was performed first, the artificial chordae was placed in the usual manner, and then a flexible annular ring was tied on the mitral annulus. An aortotomy was performed to expose the native chordae and to calibrate the length of the artificial chordae that were locked but not tied down. The mitral valve was inspected through the atriotomy while saline water was injected through the aortotomy in the left ventricle. The chordae were tied from the aortotomy and the incisions closed in the usual fashion. In the posterior leaflet prolapse, repair was done and a ring was inserted using a classical atrial incision. The ascending aorta was opened and the artificial chordae were set on the papillary muscles and the anterior leaflet was calibrated. The valve was re-inspected through the atriotomy with instillation of saline in the left ventricle for adjusting the chordae until they were definitively secured Figure 11.

#### Fig. 11.

16 Front Lines of Thoracic Surgery

**Tomita, 2005** repaired chordae tendinae with CV-4 e-PTFE sutures. Double armed sutures are passed twice through the fibrous portion of the PM head that anchors the elongated or ruptured chordae and are tied down (7 or 8 knots are needed for this suture material). The two arms of the suture are brought up to the free margin of the leaflet and passed through the point where the original chorda was attached (thickened portion of the leaflet). The needle is brought from the ventricular side of the leaflet to its atrial side and passed once more through the leaflet. The length of PTFE chordae is adjusted by referring the contact area of the opposite leaflet and then both ends of the suture are passed through the leaflet again and tied together on the ventricular side. When the prolapsed portion became wide, another PTFE suture was placed in the same fashion. At the end Kay's suture annuloplasty (n = 24) or ring annuloplasty was performed. **Minami, 2005** used double armed mattress sutures of 4-0, 5- 0 or 6-0 e-PTFE placed to reinforce with felt pledgets between the PM and free margin of the anterior leaflet. The length of the PTFE sutures was adjusted with the adjacent normal anterior leaflet or facing posterior leaflet. When the prolapsed portion became wide, another suture was placed in the same fashion. The number of sutures ranged

**Matsui, 2005** employed a new device (Matsuda Ika-Kogyo, Tokyo, Japan) consisting of two metallic tubes with a circular, hook shaped distal tip made entirely of stainless steel. The distal tip, which is perpendicularly attached to the inner tube, was designed to hold the Gore-Tex thread at the reference point on the PM immovable. The outer tube could slide on the surface of the inner tube to measure the length from the tip of inner tube to the hook of outer tube. A 4-0 or 5-0 Gore-Tex mattress suture, reinforced with a felt pledget, was placed into the head of the PM. Both arms of the suture where left untied. Length was determined by measuring the distance between the leaflet edge and the site of implantation of the artificial chordae on the PM, using a normal valve segment adjacent to the prolapsing segment as a reference. The distal tip of the inner tube of the device was placed at the sutured site of the artificial chordae on the PM. The proximal hook of the outer tube was slid to the edge of the adjacent non prolapsing leaflet and then fixed at that point after reading the distance between the distal tip and proximal hook to the device. Devices were then moved to the prolapsed segments so as to hold an edge of the prolapsed leaflet with a proximal hook. As the determined distance and edge of the leaflet were fixed with the device, the Gore-Tex suture could be tied in the usual manner without knot slipping. The action of knot-tying itself works to immobilize the device by its strength. After removing the device, followed by saline testing, a Carpenter- Edwards annuloplasty ring was attached

from 1 to 3. In addition, Kay annuloplasty was perfomed.

according to the size of the mitral annulus (Figure 10).

Fig. 10.

**Lawrie et al., 2006** published their experience on 152 consecutive patients. 5-0 PTFE sutures were placed into the bases of the papillary muscles in a figure-8 fashion, and were brought through the free edge of the prolapsing segment. Dots were made to mark the desired final line of leaflet apposition. The left ventricle was inflated with saline solution and the chordal length was adjusted to align the edges of the leaflets. Leaflet alignment was checked and the PTFE was tied down. The knot was locked with a 6-0 polypropylene stitch which was tied over the end of the PTFE to prevent sliding of the PTFE knots. An annuloplasty ring was then implanted.

**Calafiore, 2006** in the anterior leaflet prolapse passed 4-0 PTFE sutures through the fibrous tip of the papillary muscle and fixed the sutures. The new chorda was passed in the border of the anterior leaflet in the proper place and its final length was measured with a ruler. A mark was applied to indicate this distance and the suture was tied with the aid of a nerve hook (Figure 12).

Mitral Valve Subvalvular Apparatus Repair with Artificial Neochords Application 19

which was now ready to be secured to the papillary muscle. The ePTFE chordae were secured at the tip of the papillary muscle with two pledgets and attached to the edge of the

**Mandegar, 2007** for any leaflet prolapse used following technique. During preoperative transesophageal echocardiography, a line was drawn between the base of the anterior and posterior mitral leaflet to measure the distance between the head of the posterior papillary muscle and the plane at the co-optation of the leaflets; this measured the artificial chordal length. During surgery, 4-0 Gore- Tex was passed through the fibrous tip of the papillary muscle with a pledget and was fixed with a loose knot. Two tight reverse knots were made for every millimeter of 4-0 Gore-Tex that was required. The needles were passed through the edge of the anterior leaflet at the prolapsing portion, and the Gore-Tex was knotted onto a strip of pericardium so that the final knot could be placed at the atrial side of the leaflet

prolapsing mitral leaflet using eight 5-0 ePTFE sutures (Figure 14).

Fig. 14.

(Figure 15).

Fig. 15.

#### Fig. 12.

**Rankin, 2006** in the anterior and/or posterior leaflet prolapsed placed 4-0 prolene pledgetted horizontal mattress sutures longitudinally into each papillary muscle, passing one arm through the fibrous tip, and tying firmly; through this anchor suture, a doublearmed Gortex suture was passed but not tied. A Carpentier annuloplasty ring was sutured. With the ring in position, the chordae were retrieved from the ventricle, and both needles were woven into the prolapsing segment, straddling the point of maximal prolapse. Two or three bites were taken through the coaptation surface to the line of coaptation. The two arms of the suture were tied on the atrial surface with a slip-knot to bring the leaflet to the annular plane, and a clip was placed across the knot. Pericardial pledgets could be used if the leaflet tissue seems fragile. Cold saline solution was infused to check the length of the suture; once the valve was competent, eight more knot were tied tightly against the clip, the suture was cut, and the clip was removed (Figure 13).

#### Fig. 13.

**Tam, 2006** used the following technique for any prolapsing segment. A calliper was used to measure the length of the reference chordae. A 4-0 ePTFE suture was used to create loops around the calliper. Non-sliding knots were placed at the end of each loop while still on the calliper. After making a desired number of loops, the needles were passed through the loops and tied. Two needles at the end of the sutures were passed through an ePTFE pledget, which was now ready to be secured to the papillary muscle. The ePTFE chordae were secured at the tip of the papillary muscle with two pledgets and attached to the edge of the prolapsing mitral leaflet using eight 5-0 ePTFE sutures (Figure 14).

#### Fig. 14.

18 Front Lines of Thoracic Surgery

**Rankin, 2006** in the anterior and/or posterior leaflet prolapsed placed 4-0 prolene pledgetted horizontal mattress sutures longitudinally into each papillary muscle, passing one arm through the fibrous tip, and tying firmly; through this anchor suture, a doublearmed Gortex suture was passed but not tied. A Carpentier annuloplasty ring was sutured. With the ring in position, the chordae were retrieved from the ventricle, and both needles were woven into the prolapsing segment, straddling the point of maximal prolapse. Two or three bites were taken through the coaptation surface to the line of coaptation. The two arms of the suture were tied on the atrial surface with a slip-knot to bring the leaflet to the annular plane, and a clip was placed across the knot. Pericardial pledgets could be used if the leaflet tissue seems fragile. Cold saline solution was infused to check the length of the suture; once the valve was competent, eight more knot were tied tightly against the clip, the

**Tam, 2006** used the following technique for any prolapsing segment. A calliper was used to measure the length of the reference chordae. A 4-0 ePTFE suture was used to create loops around the calliper. Non-sliding knots were placed at the end of each loop while still on the calliper. After making a desired number of loops, the needles were passed through the loops and tied. Two needles at the end of the sutures were passed through an ePTFE pledget,

suture was cut, and the clip was removed (Figure 13).

Fig. 12.

Fig. 13.

**Mandegar, 2007** for any leaflet prolapse used following technique. During preoperative transesophageal echocardiography, a line was drawn between the base of the anterior and posterior mitral leaflet to measure the distance between the head of the posterior papillary muscle and the plane at the co-optation of the leaflets; this measured the artificial chordal length. During surgery, 4-0 Gore- Tex was passed through the fibrous tip of the papillary muscle with a pledget and was fixed with a loose knot. Two tight reverse knots were made for every millimeter of 4-0 Gore-Tex that was required. The needles were passed through the edge of the anterior leaflet at the prolapsing portion, and the Gore-Tex was knotted onto a strip of pericardium so that the final knot could be placed at the atrial side of the leaflet (Figure 15).

Mitral Valve Subvalvular Apparatus Repair with Artificial Neochords Application 21

half the circumference. Given a circumference of approximately 4 cm for a circle with a diameter of 13 mm the length of the loop would be approximately 2 cm. Papillary component was obtained by cutting a CV-4 PTFE suture in 2 halves; 5 double knots were placed at a distance of 2 mm at the needleless tip of each CV-4 semisuture. The needleless tip of the suture was anchored on a drape; knots were placed with the help of forceps and a needle holder and slid into definitive position by inserting the tip of the needle or a nerve hook in the knot itself. To realize papillary component for each neochorda 2 CV-4 half sutures with knots were needed. After the assessment of the mitral valve lesions, the papillary component was set in place by first fixing 2 semisutures to a papillary head and tying the sutures so that the papillary head was "sandwiched" between 2 e-PTFE pledgets to reduce trauma. Two CV-5 loops were fixed on the desired leaflet 2 to 3 mm apart from the leaflet's edge, passing the needle from the atrial to the ventricular side and leaving knots on the ventricular aspect of the leaflet. A single PTFE pledget was interposed on the atrial side. To obtain reversible coupling of the leaflet component with the papillary, a loop which could be tightened and loosened as many times as required, was placed in the leaflet component with the help of forceps and a curved instrument. The papillary component passed inside the loop and the loop was tightened. The loop had to fall in the gap between two knots. Chordal length was fixed by closing the loop under the selected reference knot of the papillary component. Same steps were repeated for the other chordae. To shorten or elongate the neochorda without touching its papillary or leaflet anchoring, the loop was released and slid under a reference knot respectively closer or farther from the papillary

**Boon et al., 2007** used CV-5 e-PTFE sutures for older children, while CV-7 was typically used in neonates and small infants. The suture was first tied to the fibrous tip of the PM and the two ends were fixed to the free edge of the valve leaflet in a V-shape. For the anterior leaflet, a new chord length was measured by bringing the free edge of the valve to the level of the anterior annulus. The length could also be compared to healthy nonelongated native chords in the adjacent area. Then both ends of the sutures would be passed again through the free edge and tied on the ventricular side of the leaflet, to prevent the knot from interfering with the co-optation zone. Because the sutures are placed in a V-shape, one suture accounts for 2 new artificial chords. In addition, ring

muscle tip, and tightened again (Figure 18).

Fig. 18.

**Gillinov, 2007** describe a technique for reparing anterior leaflet prolapse. Chordal length was determined with a calliper, and ePTFE chordae were constructed making loops around it. A pledget was used to prepare the number of 5-0 ePTFE loops that were needed. When all chordal loops were constructed, each needle was passed through the head of the papillary muscle, and was affixed to the free edge of the anterior leaflet with a figure 8 suture of CV-5 ePTFE (Figure 16).

#### Fig. 16.

**Scorsin et al., 2007**: any leaflet prolapse. Artificial chordae system device was composed of 2 sets of 4 artificial chordae, attached to a 3-mm strip of knitted polyester 18 mm wide, leaving 4 mm between each chorda. The device was applied by suturing the strip to the free edge of the prolapsed leaflet by continuous suture. Each array was anchored to the tip of the correspondent papillary muscle by only one stitch. After this procedure, a prosthetic annuloplasty ring was inserted (Figure 17).

#### Fig. 17.

**Maselli and De Paulis, 2007** used a novel system to repair the valve consisting of two components: leaflet component and the papillary component. The first one was achieved with a CV-5 PTFE suture. A circular loop was obtained at the middle of the suture by tying it around a Hegar dilator with a diameter of 13 mm. Flattened loop's length should equals half the circumference. Given a circumference of approximately 4 cm for a circle with a diameter of 13 mm the length of the loop would be approximately 2 cm. Papillary component was obtained by cutting a CV-4 PTFE suture in 2 halves; 5 double knots were placed at a distance of 2 mm at the needleless tip of each CV-4 semisuture. The needleless tip of the suture was anchored on a drape; knots were placed with the help of forceps and a needle holder and slid into definitive position by inserting the tip of the needle or a nerve hook in the knot itself. To realize papillary component for each neochorda 2 CV-4 half sutures with knots were needed. After the assessment of the mitral valve lesions, the papillary component was set in place by first fixing 2 semisutures to a papillary head and tying the sutures so that the papillary head was "sandwiched" between 2 e-PTFE pledgets to reduce trauma. Two CV-5 loops were fixed on the desired leaflet 2 to 3 mm apart from the leaflet's edge, passing the needle from the atrial to the ventricular side and leaving knots on the ventricular aspect of the leaflet. A single PTFE pledget was interposed on the atrial side. To obtain reversible coupling of the leaflet component with the papillary, a loop which could be tightened and loosened as many times as required, was placed in the leaflet component with the help of forceps and a curved instrument. The papillary component passed inside the loop and the loop was tightened. The loop had to fall in the gap between two knots. Chordal length was fixed by closing the loop under the selected reference knot of the papillary component. Same steps were repeated for the other chordae. To shorten or elongate the neochorda without touching its papillary or leaflet anchoring, the loop was released and slid under a reference knot respectively closer or farther from the papillary muscle tip, and tightened again (Figure 18).

#### Fig. 18.

20 Front Lines of Thoracic Surgery

**Gillinov, 2007** describe a technique for reparing anterior leaflet prolapse. Chordal length was determined with a calliper, and ePTFE chordae were constructed making loops around it. A pledget was used to prepare the number of 5-0 ePTFE loops that were needed. When all chordal loops were constructed, each needle was passed through the head of the papillary muscle, and was affixed to the free edge of the anterior leaflet with a figure 8 suture of CV-5

**Scorsin et al., 2007**: any leaflet prolapse. Artificial chordae system device was composed of 2 sets of 4 artificial chordae, attached to a 3-mm strip of knitted polyester 18 mm wide, leaving 4 mm between each chorda. The device was applied by suturing the strip to the free edge of the prolapsed leaflet by continuous suture. Each array was anchored to the tip of the correspondent papillary muscle by only one stitch. After this procedure, a prosthetic

**Maselli and De Paulis, 2007** used a novel system to repair the valve consisting of two components: leaflet component and the papillary component. The first one was achieved with a CV-5 PTFE suture. A circular loop was obtained at the middle of the suture by tying it around a Hegar dilator with a diameter of 13 mm. Flattened loop's length should equals

ePTFE (Figure 16).

Fig. 16.

Fig. 17.

annuloplasty ring was inserted (Figure 17).

**Boon et al., 2007** used CV-5 e-PTFE sutures for older children, while CV-7 was typically used in neonates and small infants. The suture was first tied to the fibrous tip of the PM and the two ends were fixed to the free edge of the valve leaflet in a V-shape. For the anterior leaflet, a new chord length was measured by bringing the free edge of the valve to the level of the anterior annulus. The length could also be compared to healthy nonelongated native chords in the adjacent area. Then both ends of the sutures would be passed again through the free edge and tied on the ventricular side of the leaflet, to prevent the knot from interfering with the co-optation zone. Because the sutures are placed in a V-shape, one suture accounts for 2 new artificial chords. In addition, ring

Mitral Valve Subvalvular Apparatus Repair with Artificial Neochords Application 23

**Doi, 2009** measured the length of the chordae of the posterior leaflet, opposing the prolapsing portion of the anterior leaflet by TEE. The length of chordae was a measurement of the distance between the head of the PM and the free edge of the posterior leaflet. Length of the opposing chordae of the posterior leaflet was measured directly by using a calliper. Double-armed mattress sutures with CV-5 GORE-TEX were placed at the fibrous tip of the PM using PTFE on both sides and tied down firmly. In all cases Doi performed Duran ring annuloplasty. Thereafter, the ePTFE suture is placed through the anterior leaflet. The needles were passed through the rough zone of the prolapsing portion from the atrial to the ventricular side, and again through the free margin of the leaflet from the ventricular to the atrial side. The caliper that was fixed at the length of the opposing chordae was inserted inside the loop created by the ePTFE suture. The suture was easily tied at the exact length of the opposing chordae and the anterior leaflet was fixed at the height of the posterior leaflet

The main difference between the techniques is in the measurement of the length of the artificial chordae. The oldest and most common method to calibrate the length of the neochordae consists in filling the left ventricular cavity with saline solution. Other authors elongated the prosthetic chordae trying to approximate the coaptation area between the two mitral leaflets. Recently, a variety of different calipers that allow in some manner to check the length and to tighten the number of necessary chordae have been introduced to better

define the adequacy of the PTFE chordae implantation.

Fig. 19.

(Figure 20).

Fig. 20.

annuloplasty or Wooler-Kay bilateral commissural plication annuloplasty was performed. **Chan, 2008**: for anterior leaflet prolapse a 4-0 Gore-Tex suture with pledgets was used. The suture was first passed through the papillary muscle and secured with 6 to 8 knots. Both braids were then passed through the prolapsed leaflet edge no more than 4 mm apart. The suture was then tensed up. The non-prolapsing posterior leaflet was used to check the reference length. A single-arm rubber- protected artery forceps was clipped on the mark, and knots were tied on it.

**Salvador, 2008**: for anterior leaflet prolapse repair a e-PTFE double-armed suture (GORE-TEX CV-5) were passed through the PM with a mattress technique and reinforced with autologous pericardial pledgets (rarely, GORE-TEX pledgets), on both sides of the muscle. Each end of the suture were fixed to the free margin of the prolapsed leaflet and reinforced with a small autologous pericardial pledget or a small GORE-TEX pledget. The length of the artificial chordae was adjusted to maintain the corresponding free margin of the leaflet at the desired level in the ventricular cavity. To determine the correct length of the artificial chordae, the neochordae were tied at the end of all the other repair procedures after the ventricular cavity is filled with saline solution. **Smith and Stein, 2008**  made the first endoscopic placement of multiple pre-measured artificial chordae with Robotic assistance and nitinol clip fixation. Robotic bileaflet mitral valve repair used a more lateral approach and 5 right thoracoscopic ports, ranging in size from 8 to 20 mm. Left atriotomy was perform to expose mitral valve using a robotically controlled EndoWrist atrial retractor (Intuitive Surgical Inc.). The prolapsing segment was identified with valve hooks. The "ski-tip" style ends of the robotic retractor blades are longed into the anterior leaflet, then the atrial septum is lifted to visualize PM. The length of the artificial chordae loops were determined with the measure of the distance between the correct plane of apposition on an adjacent normal non-prolapsing segment of the mitral leaflet and the respective PM (done with a More Suture Ruler device). Artificial chordae, with 4 loops each, were constructed of 4-0 PTFE GORE-TEX per the technique by von Oppel and Mohr. A single felt pledget constructed the platform with multiple neochords of definite length extending from its base. Both free suture needles from the pledget platform were passed through the respective PM with 2 robotic large needle drivers. After the correct placement in the muscle head, the needles were retrieved and the neochordae platform was secured with extracorporeal knots tied by the assistant using a closed knot pusher. Each neochordae loop was attached to the edge of the prolapsing leaflet by applying a single-armed V60 U-clip per loop. The singlearmed U-clip was placed in the leaflet edge with a robotic large needle holder and the neochordae loop was captured in the open clip circle. The U-clip was deployed by pulling the needle off the clip portion, securing the neochordae loop to the leaflet. Additional reduction of the leaflet height could be achieved by folding the leaflet edge toward the ventricle before deploying the Uclip. The remaining loops were distributed at equal distance along the edge of the prolapsed segment by applying the same technique. After the pledget platform was secured, the 2 free suture needles were placed through the anterior prolapse. The correct apposition was confirmed with saline test. The assistan, at the patient side, tied the knots. Annuloplasty was performed at the surgeon's discretion. For concomitant left atrial ablation a SurgiFlex XL probe was applied endocardially. Lastly the heart was de-aired and the left atrium was closed with a running suture line ( Figure 19).

#### Fig. 19.

22 Front Lines of Thoracic Surgery

annuloplasty or Wooler-Kay bilateral commissural plication annuloplasty was performed. **Chan, 2008**: for anterior leaflet prolapse a 4-0 Gore-Tex suture with pledgets was used. The suture was first passed through the papillary muscle and secured with 6 to 8 knots. Both braids were then passed through the prolapsed leaflet edge no more than 4 mm apart. The suture was then tensed up. The non-prolapsing posterior leaflet was used to check the reference length. A single-arm rubber- protected artery forceps was clipped on

**Salvador, 2008**: for anterior leaflet prolapse repair a e-PTFE double-armed suture (GORE-TEX CV-5) were passed through the PM with a mattress technique and reinforced with autologous pericardial pledgets (rarely, GORE-TEX pledgets), on both sides of the muscle. Each end of the suture were fixed to the free margin of the prolapsed leaflet and reinforced with a small autologous pericardial pledget or a small GORE-TEX pledget. The length of the artificial chordae was adjusted to maintain the corresponding free margin of the leaflet at the desired level in the ventricular cavity. To determine the correct length of the artificial chordae, the neochordae were tied at the end of all the other repair procedures after the ventricular cavity is filled with saline solution. **Smith and Stein, 2008**  made the first endoscopic placement of multiple pre-measured artificial chordae with Robotic assistance and nitinol clip fixation. Robotic bileaflet mitral valve repair used a more lateral approach and 5 right thoracoscopic ports, ranging in size from 8 to 20 mm. Left atriotomy was perform to expose mitral valve using a robotically controlled EndoWrist atrial retractor (Intuitive Surgical Inc.). The prolapsing segment was identified with valve hooks. The "ski-tip" style ends of the robotic retractor blades are longed into the anterior leaflet, then the atrial septum is lifted to visualize PM. The length of the artificial chordae loops were determined with the measure of the distance between the correct plane of apposition on an adjacent normal non-prolapsing segment of the mitral leaflet and the respective PM (done with a More Suture Ruler device). Artificial chordae, with 4 loops each, were constructed of 4-0 PTFE GORE-TEX per the technique by von Oppel and Mohr. A single felt pledget constructed the platform with multiple neochords of definite length extending from its base. Both free suture needles from the pledget platform were passed through the respective PM with 2 robotic large needle drivers. After the correct placement in the muscle head, the needles were retrieved and the neochordae platform was secured with extracorporeal knots tied by the assistant using a closed knot pusher. Each neochordae loop was attached to the edge of the prolapsing leaflet by applying a single-armed V60 U-clip per loop. The singlearmed U-clip was placed in the leaflet edge with a robotic large needle holder and the neochordae loop was captured in the open clip circle. The U-clip was deployed by pulling the needle off the clip portion, securing the neochordae loop to the leaflet. Additional reduction of the leaflet height could be achieved by folding the leaflet edge toward the ventricle before deploying the Uclip. The remaining loops were distributed at equal distance along the edge of the prolapsed segment by applying the same technique. After the pledget platform was secured, the 2 free suture needles were placed through the anterior prolapse. The correct apposition was confirmed with saline test. The assistan, at the patient side, tied the knots. Annuloplasty was performed at the surgeon's discretion. For concomitant left atrial ablation a SurgiFlex XL probe was applied endocardially. Lastly the heart was de-aired

and the left atrium was closed with a running suture line ( Figure 19).

the mark, and knots were tied on it.

**Doi, 2009** measured the length of the chordae of the posterior leaflet, opposing the prolapsing portion of the anterior leaflet by TEE. The length of chordae was a measurement of the distance between the head of the PM and the free edge of the posterior leaflet. Length of the opposing chordae of the posterior leaflet was measured directly by using a calliper. Double-armed mattress sutures with CV-5 GORE-TEX were placed at the fibrous tip of the PM using PTFE on both sides and tied down firmly. In all cases Doi performed Duran ring annuloplasty. Thereafter, the ePTFE suture is placed through the anterior leaflet. The needles were passed through the rough zone of the prolapsing portion from the atrial to the ventricular side, and again through the free margin of the leaflet from the ventricular to the atrial side. The caliper that was fixed at the length of the opposing chordae was inserted inside the loop created by the ePTFE suture. The suture was easily tied at the exact length of the opposing chordae and the anterior leaflet was fixed at the height of the posterior leaflet (Figure 20).

#### Fig. 20.

The main difference between the techniques is in the measurement of the length of the artificial chordae. The oldest and most common method to calibrate the length of the neochordae consists in filling the left ventricular cavity with saline solution. Other authors elongated the prosthetic chordae trying to approximate the coaptation area between the two mitral leaflets. Recently, a variety of different calipers that allow in some manner to check the length and to tighten the number of necessary chordae have been introduced to better define the adequacy of the PTFE chordae implantation.

Mitral Valve Subvalvular Apparatus Repair with Artificial Neochords Application 25

prolapse, 5 (17%) patients – bileaflet prolapse, and 18 (60%) – anterior leaflet prolapse. We performed 7 posterior mitral valve leaflet chord reconstructions, in 4 cases with multiple loops. Anterior mitral leaflet chord repair was carried out in 18 (60%) patients (including multiple loops in 10 cases). 5 (17%) patient had total AML chord repair ( Figure 22, 23).

Fig. 22. Patient Ch. Preoperative echocardiography (left, two-chambered position at the

mitral valve level) and postoperative view (right, four-chambered position).

Fig. 23. Patient Ch. Intraoperative photo total anterior mitral leaflet chord repair.
