**2.3 Analysis method**

Regarding research on serum peptidomics, the analysis of data collected shall have: highefficient analysis technical platform, computer and network have become a necessary tool of biological research; high throughput technical platform, mainly targeting at how to use the information technology to analyze the giant data; data mining technical platform, which shall be able to mine knowledge from the massive data saved in database or other information banks for the analysis; data visualization technical platform, in describing the systematic relations, the functions of nucleic acid, protein, cell, organ and tissue shall be considered, that is to say, a systematic method shall be used to learn about vital activities.

Currently, databases used for proteomics research include SWISS-PROT, BLOCKS, SMART, PROSITE, WORLD-2D-PAGE, EMBL, GenBank, DDBJ, ProClass, PR INTS, MASCOT, PROTO-MAP, DOMO, PDB and NCBI. Among them, SWISS-PROT is a real protein sequence database and also the largest and most diversified proteome database in the world. EMBL is to collect protein sequences that have been translated from nucleic acid automatically and not yet entered the SWISS-PROT. NCBI contains protein sequences translated from DNA in the GenBank and from the PDB, SWISS-PROT and PIR databases.

Presently, many tools and methods used for MS data process and analysis of serum peptidomics have been developed, mainly including:
