**2.1.10 Varying** *steady-state* **and start (baseline) concentrations**

For each algorithm, a number of steady-state and start concentrations are used (2.38, 4.75, 9.5, 14.3, 19.0, 23.8, 28.5, 38.0, 47.5, 57.0, 66.5, 76.0, 85.5, and 95.0 U/L) where the concentration in the first 'sample' is fixed at 0.1 % below the stated concentration in order to ensure that at least one sample from each 'patient' is below the cut-off threshold. The percentage of positives at a certain time/sample is illustrated as a function of the starting concentration for each algorithm (see e.g. Fig 4).
