**6. Acknowledgment**

This work was financially sponsored by the National Natural Science Foundation of China (No. 21004039), the Research Foundation for The Excellent Youth Scholars of Higher Education of Shanghai, the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry, and the Innovative Foundation of Shanghai University.

## **7. References**


This work was financially sponsored by the National Natural Science Foundation of China (No. 21004039), the Research Foundation for The Excellent Youth Scholars of Higher Education of Shanghai, the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry, and the Innovative Foundation of Shanghai

Asada, S., Fukuda, K., Oh, M., Hamanishi, C. & Tanaka, S. (1999). Effect of Hydrogen

Balakrishnan, B., Mohanty, M., Umashankar, P.R. & Jayakrishnan, A. (2005). Evaluation of

Balakrishnan, B., Mohanty, M., Fernandez, A.C., Mohanan, P.V. & Jayakrishnan, A. (2006).

Benya, P.D. & Shaffer, J.D. (1982). Dedifferentiated Chondrocytes Reexpress the

Bryant, S.J. & Anseth, K.S. (2002). Hydrogel Properties Influence ECM Production by

Bryant, S.J. & Anseth, K.S. (2003). Controlling the Spatial Distribution of ECM Components

Bryant, S.J., Durand, K.L. & Anseth, K.S. (2003). Manipulations in Hydrogel Chemistry

Bryant, S.J., Arthur, J.A. & Anseth, K.S. (2005). Incorporation of Tissue-Specific Molecules

Burdick, J.A. & Anseth, K.S. (2002). Photoencapsulation of Osteoblasts in Injectable Rgd-

Cai, S., Liu, Y., Shu, X.Z. & Prestwich, G.D. (2005). Injectable Glycosaminoglycan Hydrogels

Vol.48, No.7, (July 1999), pp. 399-403, ISSN 1023-3830

No.1, (August 1982), pp. 215-224, ISSN 0092-8674

2003), pp. 1430-1436, ISSN 1552-4965

(November 2002), pp. 4315-4323, ISSN 0142-9612

Vol.26, No.30, (October 2005), pp. 6054-6067, ISSN 0142-9612

Peroxide on the Metabolism of Articular Chondrocytes. *Inflammation Research,*

an in Situ Forming Hydrogel Wound Dressing Based on Oxidized Alginate and Gelatin. *Biomaterials,* Vol.26, No.32, (November 2005), pp. 6335-6342, ISSN 0142-

Evaluation of the Effect of Incorporation of Dibutyryl Cyclic Adenosine Monophosphate in an in Situ-Forming Hydrogel Wound Dressing Based on Oxidized Alginate and Gelatin. *Biomaterials,* Vol.27, No.8, (March 2006), pp. 1355-

Differentiated Collagen Phenotype When Cultured in Agarose Gels. *Cell,* Vol.30,

Chondrocytes Photoencapsulated in Poly(Ethylene Glycol) Hydrogels. *Journal of Biomedical Materials Research,* Vol.59, No.1, (January 2002), pp. 63-72, ISSN 1552-

in Degradable PEG Hydrogels for Tissue Engineering Cartilage. *Journal of Biomedical Materials Research Part A,* Vol.64A, No.1, (January 2003), pp. 70-79, ISSN

Control Photoencapsulated Chondrocyte Behavior and Their Extracellular Matrix Production. *Journal of Biomedical Materials Research: Part A,* Vol.67A, No.4, (June

Alters Chondrocyte Metabolism and Gene Expression in Photocrosslinked Hydrogels. *Acta Biomaterialia,* Vol.1, No.2, (March 2005), pp. 243-252, ISSN 1742-

Modified PEG Hydrogels for Bone Tissue Engineering. *Biomaterials,* Vol.23, No.22,

for Controlled Release of Human Basic Fibroblast Growth Factor *Biomaterials,*

**6. Acknowledgment** 

University.

**7. References** 

9612

4965

7061

1552-4965

1361, ISSN 0142-9612


In-Situ Forming Biomimetic Hydrogels for Tissue Regeneration 55

Jones, A. & Vaughan, D. (2005). Hydrogel Dressing in the Management of a Variety of

Khattak, S.F., Bhatia, S.R. & Roberts, S.C. (2005). Pluronic F127 as a Cell Encapsulation

Kim, M., Shin, Y., Hong, B.-H., Kim, Y.-J., Chun, J.-S., Tae, G. & Kim, Y.H. (2010). In Vitro

Kobayashi, S., Uyama, H. & Kimura, S. (2001). Enzymatic Polymerization. *Chemical Review,*

Kuo, C.K. & Ma, P.X. (2001). Ionically Crosslinked Alginate Hydrogels as Scaffolds for

Lee, F., Chung, J.E. & Kurisawa, M. (2009). An Injectable Hyaluronic Acid-Tyramine

Lee, H. & Park, T.G. (2009). Photo-Crosslinkable, Biomimetic, and Thermo-Sensitive

Lee, H.J., Lee, J.-S., Chansakul, T., Yu, C., Elisseeff, J.H. & Yu, S.M. (2006). Collagen Mimetic

Lee, H.J., Yu, C., Chansakul, T., Hwang, N.S., Varghese, S., Yu, S.M. & Elisseeff, J.H. (2008).

Lee, S.H. & Shin, H. (2007). Matrices and Scaffolds for Delivery of Bioactive Molecules in

Liu, Y., Shu, X.Z. & Prestwich, G.D. (2006). Osteochondral Defect Repair with Autologous

Lutolf, M.P., Raeber, G.P., Zisch, A.H., Tirelli, N. & Hubbell, J.A. (2003). Cell-Responsive

Martens, P.J., Bryant, S.J. & Anseth, K.S. (2003). Tailoring the Degradation of Hydrogels

Vol.101, No.12, (November 2001), pp. 3793-3818, ISSN 0009-2665

*Biomaterials,* Vol.22, No.6, (March 2001), pp. 511-521, ISSN 0142-9612 Langer, R. & Vacanti, J.P. (1993). Tissue Engineering. *Science,* Vol.260, No.5110, (May 1993),

2005), pp. S1-S11, ISSN 1361-3111

pp. 920-926, ISSN 0036-8075

No.5-6, (May-June 2005), pp. 974-983, ISSN 1076-3279

(November 1999), pp. 183-190, ISSN 0144-8617

(March 2009), pp. 186-193, ISSN 0168-3659

No.30, (October 2006), pp. 5268-5276, ISSN 0142-9612

(November 2008), pp. 1843-1851, ISSN 1076-3279

No.4-5, (May 2007), pp. 339-359, ISSN 0169-409X

2006), pp. 3405-3416, ISSN 1076-3279

ISSN 0935-9648

2009), pp. 797-806, ISSN 1552-4965

Wound Types: A Review. *Journal of Orthopaedic Nursing,* Vol.9, No.1, (December

Material: Utilization of Membrane-Stabilizing Agents. *Tissue engineering,* Vol.11,

Chondrocyte Culture in a Heparin-Based Hydrogel for Cartilage Regeneration. *Tissue Engineering Part C,* Vol.16, No.1, (February 2010), pp. 1-10, ISSN 1076-3279 Kim, S.H., Won, C.Y. & Chu, C.C. (1999). Synthesis and Characterization of Dextran-Based

Hydrogel Prepared by Photocrosslinking. *Carbohydrate Polymers,* Vol.40, No.3,

Tissue Engineering: Part I. Structure, Gelation Rate and Mechanical Properties.

Hydrogel System for Protein Delivery. *Journal of Controlled Release,* Vol.134, No.3,

Pluronic Grafted Hyaluronic Acid Copolymers for Injectable Delivery of Chondrocytes. *Journal of Biomedical Materials Research Part A,* Vol.88A, No.3, (March

Peptide-Conjugated Photopolymerizable PEG Hydrogel. *Biomaterials,* Vol.27,

Enhanced Chondrogenesis of Mesenchymal Stem Cells in Collagen Mimetic Peptide-Mediated Microenvironment. *Tissue Engineering Part A,* Vol.14, No.11,

Bone and Cartilage Tissue Engineering. *Advanced Drug Delivery Review,* Vol.59,

Bone Marrow-Derived Mesenchymal Stem Cells in an Injectable, in Situ, Cross-Linked Synthetic Extracellular Matrix. *Tissue engineering,* Vol.12, No.12, (December

Synthetic Hydrogels. *Advanced Materials,* Vol.15, No.11, (June 2003), pp. 888-892,

Formed from Multivinyl Poly(ethylene glycol) and Poly(vinyl alcohol) Macromers

Synthesis, Rheology, and Degradation. *Macromolecules,* Vol.40, No.4, (January 2007a), pp. 1165-1173, ISSN 0024-9297


Hiemstra, C., Zhou, W., Zhong, Z., Wouters, M. & Feijen, J. (2007b). Rapidly in Situ Forming

Hoemann, C.D., Sun, J., Légaré, A., McKee, M.D. & Buschmann, M.D. (2005). Tissue

Holland, T.A., Tabata, Y. & Mikos, A.G. (2003). In Vitro Release of Transforming Growth

*Release,* Vol.91, No.3, (September 2003), pp. 299-313, ISSN 0168-3659 Hu, B.-H. & Messersmith, P.B. (2003). Rational Design of Transglutaminase Substrate

2007a), pp. 1165-1173, ISSN 0024-9297

ISSN 1063-4584

313, ISSN 0168-3659

ISSN 0024-9297

862, ISSN 0028-0836

(October 2007), pp. 2369-2385, 1076-3279

1999), pp. 7064-7069, ISSN 0024-9297

2007), pp. 2791-2800, ISSN 0142-9612

(August 2007b), pp. 9918-9926, ISSN 0002-7863

Synthesis, Rheology, and Degradation. *Macromolecules,* Vol.40, No.4, (January

Biodegradable Robust Hydrogels by Combining Stereocomplexation and Photopolymerization. *Journal of the American Chemical Society,* Vol.129, No.32,

Engineering of Cartilage Using an Injectable and Adhesive Chitosan-Based Cell-Delivery Vehicle. *Osteoarthritis and Cartilage,* Vol.13, No.4, (April 2005), pp. 318-329,

Factor-[Beta]1 from Gelatin Microparticles Encapsulated in Biodegradable, Injectable Oligo(Poly(Ethylene Glycol) Fumarate) Hydrogels. *Journal of Controlled* 

Peptides for Rapid Enzymatic Formation of Hydrogels. *Journal of the American Chemical Soiety,* Vol.125, No.47, (October 2003), pp. 14298-14299, ISSN 0002-7863 Hubbell, J.A. (1996). Hydrogel Systems for Barriers and Local Drug Delivery in the Control

of Wound Healing. *Journal of Controlled Release,* Vol.39, No.2-3, (May 1996), pp. 305-

Biomaterials for Tissue Engineering Applications. *Tissue engineering,* Vol.13, No.10,

Enantiomeric Poly(Lactide). *Macromolecules,* Vol.20, No.4, (April 1987), pp. 904-906,

Hattori, H., Uenoyama, M. & Kurita, A. (2002). Photocrosslinkable Chitosan as a Dressing for Wound Occlusion and Accelerator in Healing Process. *Biomaterials,*

Injectable Drug-Delivery Systems. *Nature,* Vol.388, No.1, (August 1997), pp. 860-

Triblock Copolymer Aqueous Solutions. *Macromolecules,* Vol.32, No.21, (October

Copolymer Aqueous Solutions and Degradation Thereof. *Journal of Biomedical* 

of Hydrogels from Dextran-Tyramine Conjugates. *Biomaterials,* Vol.28, No.18, (June

& Feijen, J. (2009). Injectable Chitosan-Based Hydrogels for Cartilage Tissue Engineering. *Biomaterials,* Vol.30, No.13, (May 2009), pp. 2544-2551, ISSN 0142-9612

Ifkovits, J.L. & Burdick, J.A. (2007). Review: Photopolymerizable and Degradable

Ikada, Y., K.Jamshidi, Tsuji, H. & Hyon, S.-H. (1987). Stereocomplex Formation between

Ishihara, M., Nakanishi, K., Ono, K., Sato, M., Kikuchi, M., Saito, Y., Yura, H., Matsui, T.,

Jeong, B., Bae, Y.H., Lee, D.S. & Kim, S.W. (1997). Biodegradable Block Copolymers as

Jeong, B., Bae, Y.H. & Kim, S.W. (1999). Thermoreversible Gelation of PEG-PLGA-PEG

Jeong, B., Bae, Y.H. & Kim, S.W. (2000). In Situ Gelation of PEG-PLGA-PEG Triblock

Jin, R., Moreira Teixeira, L.S., Dijkstra, P.J., Karperien, M., van Blitterswijk, C.A., Zhong, Z.Y.

*Materials Research,* Vol.50, No.2, (May 2000), pp. 171-177, ISSN 1549-3296 Jin, R., Hiemstra, C., Zhong, Z. & Feijen, J. (2007). Enzyme-Mediated Fast in Situ Formation

Vol.23, No.3, (February 2002), pp. 833-840, ISSN 0142-9612


In-Situ Forming Biomimetic Hydrogels for Tissue Regeneration 57

Seliktar, D., Zisch, A.H., Lutolf, M.P., Wrana, J.L. & Hubbell, J.A. (2004). Mmp-2 Sensitive,

Silver, D., Kapsch, D.N. & Tsoi, E.K. (1983). Heparin-Induced Thrombocytopenia,

Skjak-Brvk, G., Grasdalen, H. & Smidsrod, O. (1989). Inhomogeneous Polysaccharide Ionic

Sontjens, S.H.M., Nettles, D.L., Carnahan, M.A., Setton, L.A. & Grinstaff, M.W. (2006).

*Biomacromolecules,* Vol.7, No.1, (January 2006), pp. 310-316, ISSN 1525-7797 Sperinde, J.J. & Griffith, L.G. (1997). Synthesis and Characterization of Enzymatically-Cross-

Tomme, S.R.v., Steenbergen, M.J.v., Smedt, S.C.d., Nostrum, C.F.v. & Hennink, W.E. (2005).

Wang, D.A., Williams, C.G., Yang, F. & Elisseeff, J.H. (2004). Enhancing the Tissue-

Wang, D.A., Varghese, S., Sharma, B., Strehin, I., Fermanian, S., Gorham, J., Fairbrother,

Wathier, M., Jung, P.J., Carnahan, M.A., Kim, T. & Grinstaff, M.W. (2004). Dendritic

Wathier, M., Johnson, C.S., Kim, T. & Grinstaff, M.W. (2006). Hydrogels Formed by Multiple

Weng, L., Gouldstone, A., Wu, Y. & Chen, W. (2008). Mechanically Strong Double Network

Wichterle, O. & Lim, D. (1960). Hydrophilic Gels in Biologic Use. *Nature,* Vol.185, No.1,

Xu, C. & Kopeček, J. (2008). Genetically Engineered Block Copolymers: Influence of the

Vol.17, No.4, (June 2006), pp. 873-876, ISSN 1043-1802

(January 1960), pp. 117-118, ISSN 0028-0836

*Biomaterials,* Vol.26, No.14, (May 2005), pp. 2129-2135, ISSN 0142-9612 Wang, C., Stewart, R.J. & KopeCek, J. (1999). Hybrid Hydrogels Assembled from Synthetic

1549-3296

pp. 301-306, ISSN 0003-4932

1997), pp. 5255-5264, ISSN 0024-9297

1999), pp. 417-420, ISSN 0028-0836

pp. 385-392, ISSN 1476-1122

12745, ISSN 0002-7863

2163, ISSN 0142-9612

Vegf-Bearing Bioactive Hydrogels for Promotion of Vascular Healing. *Journal of Biomedical Materials Research: Part A,* Vol.68, No.4, (March 2004), pp. 704-716, ISSN

Thrombosis, and Hemorrhage. *Annuals of Surgery,* Vol.198, No.3, (September 1983),

Gels. *Carbohydrate Polymer,* Vol.10, No.1, (February 1989), pp. 31-54, ISSN 0144-8617

Biodendrimer-Based Hydrogel Scaffolds for Cartilage Tissue Repair.

Linked Poly(Ethylene Glycol) Hydrogels. *Macromolecules,* Vol.30, No.(September

Self-Gelling Hydrogels Based on Oppositely Charged Dextran Microspheres.

Polymers and Coiled-Coil Protein Domains. *Nature,* Vol.397, No.6718, (February

Biomaterial Interface: Tissue-Initiated Integration of Biomaterials. *Advanced Functional Materials,* Vol.14, No.12, (December 2004), pp. 1152-1159, ISSN 1616-3028

D.H., Cascio, B. & Elisseeff, J.H. (2007). Multifunctional Chondroitin Sulphate for Cartilage Tissue-Biomaterial Integration. *Nature Materials,* Vol.6, No.5, (May 2007),

Macromers as in Situ Polymerizing Biomaterials for Securing Cataract Incisions. *Journal of the American Chemical Society,* Vol.126, No.40, (September 2004), pp. 12744-

Peptide Ligation Reactions to Fasten Corneal Transplants. *Bioconjugated Chemistry,*

Photocrosslinked Hydrogels from N, N-Dimethylacrylamide and Glycidyl Methacrylated Hyaluronan. *Biomaterials,* Vol.29, No.14, (February 2008), pp. 2153-

Length and Structure of the Coiled-Coil Blocks on Hydrogel Self-Assembly. *Pharmaceutical Research,* Vol.25, No.3, (March 2008), pp. 674-682, ISSN 0724-8741

for Cartilage Tissue Engineering. *Biomacromolecules,* Vol.4, No.2, (March-April 2003), pp. 283-292, ISSN 1525-7797


Mather, B.D., Viswanathan, K., Miller, K.M. & Long, T.E. (2006). Michael Addition Reactions

McHale, M.K., Setton, L.A. & Chilkoti, A. (2005). Synthesis and in Vitro Evaluation of

Nettles, D.L., Kitaoka, K., Hanson, N.A., Flahiff, C.M., Mata, B.A., Hsu, E.W., Chilkoti, A. &

Obara, K., Ishihara, M., Ishizuka, T., Fujita, M., Ozeki, Y., Maehara, T., Saito, Y., Yura, H.,

Ono, K., Saito, Y., Yura, H., Ishikawa, K., Kurita, A., Akaike, T. & Ishihara, M. (2000).

Park, K.H. & Na, K. (2008). Effect of Growth Factors on Chondrogenic Differentiation of

Park, Y.D., Tirelli, N. & Hubbell, J.A. (2003). Photopolymerized Hyaluronic Acid-Based

Park, Y.D., Lutolf, M.P., Hubbell, J.A., Hunziker, E.B. & Wong, M. (2004). Bovine Primary

*engineering,* Vol.10, No.3-4, (March-April 2004), pp. 515-522, ISSN 1076-3279 Sanborn, T.J., Messersmith, P.B. & Barron, A.E. (2002). In Situ Crosslinking of a Biomimetic

*Biomaterials,* Vol.23, No.13, (July 2002), pp. 2703-2710, ISSN 0142-9612

*and Bioengineering,* Vol.106, No.1, (July 2008), pp. 74-79, ISSN 1389-1723 Park, Y., Sugimoto, M., Watrin, A., Chiquet, M. & Hunziker, E.B. (2005). BMP-2 Induces the

Vol.13, No.6, (June 2005), pp. 527-536, ISSN 1063-4584

*Research,* Vol.49, No.2, (February 2000), pp. 289-295, ISSN 1549-3296 Park, H., Temenoff, J.S., Holland, T.A., Tabata, Y. & Mikos, A.G. (2005). Delivery of TGF-

2003), pp. 283-292, ISSN 1525-7797

2003), pp. 3437-3444, ISSN 0142-9612

7095-7103, ISSN 0142-9612

2003), pp. 893-900, ISSN 0142-9612

1076-3279

0142-9612

Vol.31, No.5, (May 2006), pp. 487-531, ISSN 0079-6700

for Cartilage Tissue Engineering. *Biomacromolecules,* Vol.4, No.2, (March-April

in Macromolecular Design for Emerging Technologies. *Progress in Polymer Science,*

Enzymatically Cross-Linked Elastin-Like Polypeptide Gels for Cartilaginous Tissue Repair. *Tissue Engingeering,* Vol.11, No.11-12, (January 2005), pp. 1768-1779, ISSN

Setton, L.A. (2008). In Situ Crosslinking Elastin-Like Polypeptide Gels for Application to Articular Cartilage Repair in a Goat Osteochondral Defect Model. *Tissue Engineering Part A,* Vol.14, No.7, (July 2008), pp. 1133-1140, ISSN 1076-3279 Nguyen, K.T. & West, J.L. (2002). Photopolymerizable Hydrogels for Tissue Engineering

Applications. *Biomaterials,* Vol.23, No.22, (November 2002), pp. 4307-4314, ISSN

Matsui, T., Hattori, H., Kikuchi, M. & Kurita, A. (2003). Photocrosslinkable Chitosan Hydrogel Containing Fibroblast Growth Factor-2 Stimulates Wound Healing in Healing-Impaired db/db Mice. *Biomaterials,* Vol.24, No.20, (September

Photocrosslinkable Chitosan as a Biological Adhesive. *Journal of Biomedical Mterials* 

Beta1 and Chondrocytes Via Injectable, Biodegradable Hydrogels for Cartilage Tissue Engineering Applications. *Biomaterials,* Vol.26, No.34, (December 2005), pp.

Rabbit Mesenchymal Cells Embedded in Injectable Hydrogels. *Journal of Bioscience* 

Expression of Chondrocyte-Specific Genes in Bovine Synovium-Derived Progenitor Cells Cultured in Three-Dimensional Alginate Hydrogel. *Osteoarthritis Cartilage,*

Hydrogels and Interpenetrating Networks. *Biomaterials,* Vol.24, No.10, (March

Chondrocyte Culture in Synthetic Matrix Metalloproteinase-Sensitive Poly(ethylene glycol)-Based Hydrogels as a Scaffold for Cartilage Repair. *Tissue* 

Peptide-PEG Hydrogel Via Thermally Triggered Activation of Factor XIII


**Part 2** 

**Gene Medicine and Nanobiomedicine** 

