**5. Conclusion**

Novel crosslinking methods provide significant opportunities for the design of in-situ forming hydrogels with multifunctional properties on demand for tissue regeneration. The fast progress in molecular biology inspires researchers to design biomimetic in situ forming hydrogels. Polymer composition and structures, hydrogel forming methods, degradation properties, mechanical strength and biocompatibility are of significant importance. Artificial extracellular matrices combining in situ forming hydrogel scaffolds, cells and growth factors hold great promise for tissue engineering, and pave the way for regenerated tissue.

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