**2.2.3 Disaccharidase activity**

The lactase (EC 3.2.1.23), maltase (EC 3.2.1.20) and saccharase (EC 3.2.1.26) activities were measured according to Mir et al. (1997). Mucosa samples (200 mg) were homogenized for 3 min with 1 mL saline solution at 0oC. The homogenate was transferred to a test tube together with 2.5 mL (2 × aliquot) of saline solution. Three reaction tubes were filled with 100 µL of the homogenate and placed in a 37oC water bath, and then 400 µL of 56 mM lactose, maltose, saccharose in citrate buffer (pH 6.6, 0.01 mM) were added, respectively. After shaking and incubation for 30 min enzyme activity was stopped in boiling water. The reaction tubes were centrifuged at 2000 × g (30 min, 5oC). The individual enzymes were determined using enzymatic UV method (Boehringer Mannheim, Germany). Protein content in homogenate was started according to Bradford et al. (1976) and the results were expressed as µmol/ mg protein/ hour.
