**2.2.5 Statistical analysis**

Statistical analysis was performed using Statistic software PRIZMA (version 3.0). All the data were presented as means ± SEM. To estimate the effect of age and weaning on the concentration of SCFAs, bacterial count, disaccharidase activity and intestinal morphology, the data were evaluated statistically by one-way analysis of variance (ANOVA) followed by a multiple comparison Tukey´s test. Significant differences between the two groups of piglets were tested using analysis of variance and Student´s t-test. Probability values less than 0.05 were used as the criterion for statistical significance.
