**5. Conclusions**

Flavonoids, having a wide spectrum of health-beneficial activities, seem to be applicable in various areas of national management from food additivization to pharmaceutical preparations with the purpose of prevention and/or treatment of important civilization diseases. Their chemical structure determines not only biological effects on human health but also their solubility, stability and bioavailability. Recently, selective enzyme-mediated acylation of flavonoids has been introduced to confer improved biological properties to the novel compounds including both biological activity of initial flavonoid and other parameters determined by the chemical structure of an acyl donor. In the past, proteases, esterases and acyltransferases were used for the preparation of acylated flavonoids. In light of our review, immobilized lipases, especially *Candida antarctica* B lipase, are suitable for this purpose. Not only the given enzyme but also the reaction conditions have a distinct influence on the performance of acylation. This aspect must be considered when producing acylated flavonoids in technology scale for potential uses in the food, pharmaceutical and cosmetic industry.

#### **6. Acknowledgment**

The work was supported by The Slovak Research and Development Agency in the frame of the Project APVV-VMSP-II-0021-09 and by The Agency of the Ministry of Education, Science, Research and Sport of the Slovak Republic for the Structural Funds of EU, OP R&D of ERDF in the frame of the Project "Evaluation of natural substances and their selection for prevention and treatment of lifestyle diseases (ITMS 26240220040).

#### **7. References**


anti-wrinkle agents, stimulators of the synthesis of the components of the extracellular matrix,

Flavonoids, having a wide spectrum of health-beneficial activities, seem to be applicable in various areas of national management from food additivization to pharmaceutical preparations with the purpose of prevention and/or treatment of important civilization diseases. Their chemical structure determines not only biological effects on human health but also their solubility, stability and bioavailability. Recently, selective enzyme-mediated acylation of flavonoids has been introduced to confer improved biological properties to the novel compounds including both biological activity of initial flavonoid and other parameters determined by the chemical structure of an acyl donor. In the past, proteases, esterases and acyltransferases were used for the preparation of acylated flavonoids. In light of our review, immobilized lipases, especially *Candida antarctica* B lipase, are suitable for this purpose. Not only the given enzyme but also the reaction conditions have a distinct influence on the performance of acylation. This aspect must be considered when producing acylated flavonoids in technology scale for potential uses in the food, pharmaceutical and cosmetic industry.

The work was supported by The Slovak Research and Development Agency in the frame of the Project APVV-VMSP-II-0021-09 and by The Agency of the Ministry of Education, Science, Research and Sport of the Slovak Republic for the Structural Funds of EU, OP R&D of ERDF in the frame of the Project "Evaluation of natural substances and their selection for

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