**4.1 Primers selection**

Band patterns variation may be related to high occurrence rate of base substitution and the great possibility of indels accumulation (events of inserts and/or deletions of nucleotides), moreover, these sequences are difficult to identify (Albert *et al*., 2002). The study with a great number of genotypes aims to explain the inheritance of the chloroplast, which may vary according to the subgenus and be useful for genetic diversity studies of the group. These *primers* (*trnL-F*) have been successfully used in genetic diversity analysis of Orchidaceae (Kocyan *et al*., 2004) and Bromeliaceae groups (Sousa *et al*., 2007).
