**2.4 Hematoxylin-Eosin (HE) and Masson's Trichrome (TRI) histopathological preparations**

For microscopic preparations, after 15 days, 3 surviving fish of each group were removed and dissected. Small pieces of the liver, kidneys, gills, and skin were taken and immediately fixed in 10% neutral buffered formalin. Fixed tissues were processed routinely for paraffin embedding technique. Embedded tissues were sectioned at 5-7μ in thickness and then stained with Harris' hematoxylin and eosin stain (H & E) and Masson's Trichrome (TRI) stain according to Bancroft and Steven, (1982). Sections were visualized and studied using OLYMPUS microscope model BX50F4 (Olympus optical Co., LTP. Japan).
