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**26** 

*China* 

Jiang-hua Ran

*The First Hospital of Kunming* 

 *and The Ganmei Hospital Affiliated of Kunming Medical College Kunming,* 

**Causes of Death of Rhesus Monkeys** 

As non-human primates have similar pathophysiological reactions to humans, experimental data evaluating acute rejection reactions following liver transplantation in rhesus monkeys are clinically significant. However, the success rate and long-term survival rate are low, and post-transplant death is one of the major factors influencing survival in rhesus monkeys undergoing experimental liver transplantation.Non-human primates provide the ideal model for clinical studies of liver transplantation. A large number of factors can influence the establishment of a stable and reproducible non-human primate model. Post-transplant death is a major problem in experimental liver transplantation in rhesus monkeys. This

Healthy rhesus monkeys provided by the Laboratory Animal Center of Kunming Medical University were used as donor and recipient. Recipients were male rhesus monkeys weighing 7.2–11.5 kg, and donors were of either gender weighing 5.3–8.1 kg. The animals were housed in the Laboratory Animal Center of Kunming Medical University, and were allowed free access to food and water. Food access for donors was restricted, and recipients were starved of food for 12 hours and of water for 6 hours preoperatively. Recipients were given cefazolin sodium (0.1 g/kg) before transplantation. Experimental procedures were performed in accordance with the Guidance Suggestions for the Care and Use of Laboratory Animals formulated by the Ministry of Science and Technology of the People's Republic of China.

We operated on 9 rhesus monkeys using our original surgical model, and then modified our model for the remaining 16 monkeys. In the original model, the hepatic vein, portal vein and hepatic artery were directly anastomosed, and a supporting tube was placed in the biliary

study investigates causes of rhesus monkey death following liver transplantation.

**1. Introduction** 

**2. Materials and methods** 

**2.2 Surgery for donor and recipient animals** 

tract. The modified model is described below.

**2.1 Materials** 

**Undergoing Liver Transplantation** 

*The Department of Hepato-Biliary-Pancreatic Surgery* 

