**2.3.1 Extraction, filtration and concentration**

The milled samples (100-500g) were soaked in 70% ethanol (5L) for 48hours with frequent shaking. Thereafter the extracts were filtered first with cotton wool followed by Whatman filter paper® and stored in at room temperature. The filtrate was then concentrated using vacuum rotary evaporator. The concentrates were then dried in vacuum oven at 60oC to dryness.
