**Meet the editor**

Dr. Christopher L. Frewin is a National Institute of Health Ruth L. Kirschstein National Research Service Post Doctoral Fellow in the Department of Molecular Pharmacology and Physiology at the University of South Florida. He began as materials scientist. He designed and generated a chemical vapor deposition reactor for the heteroepitaxial growth of cubic silicon

carbide (3C-SiC). He used the AFM at Max-Planck-Institut für Festkörperforschung, Stuttgart, Germany to evaluate topographic changes in the etched surfaces of SiC. He employed AFM techniques to characterize his 3C-SiC crystals grown in the CVD reactor. During his Ph.D. candidacy, he switched focus from materials science to bioengineering by characterizing the in vitro biocompatibility of 3C-SiC and diamond to neural cells. The key of this investigation centered on living cell AFM measurements which were used to assess cellular lamellipodia/ filopodia permissiveness. Currently he is developing 3C-SiC implantable neural prosthetics for brain machine interface devices.

Contents

**Preface IX** 

**Part 1 General Techniques 1** 

Chapter 1 **Atomic Force Spectroscopies:** 

**A Toolbox for Probing the Biological Matter 3**  Michele Giocondo, Said Houmadi, Emanuela Bruno, Maria P. De Santo, Luca De Stefano, Emmanuelle Lacaze,

Sara Longobardi and Paola Giardina

Chapter 2 **Artifacts in Atomic Force Microscopy of Biological Samples 29**  E. Ukraintsev, A. Kromka, H. Kozak,

Z. Remeš and B. Rezek

Chapter 3 **Tapping Mode AFM Imaging for** 

L. Latterini and L. Tarpani

Chapter 6 **Protein Interactions on Phospholipid Bilayer, Studied by AFM Under Physiological Conditions 123**  Špela Irman, Miha Škarabot, Igor Muševič and Borut Božič

Taiji Ikawa

Chapter 5 **AFM Imaging of Biological Supramolecules** 

**Process on a Photopolymer 99** 

**Functionalized Surfaces 55**  Nadine Mourougou-Candoni

**Part 2 Biological Molecules, Proteins and Polymers 85** 

Chapter 4 **AFM Measurements to Investigate Particulates and** 

**Their Interactions with Biological Macromolecules 87** 

**by a Molecular Imprinting-Based Immobilization** 
