**5. Acknowledgment**

114 Bioluminescence – Recent Advances in Oceanic Measurements and Laboratory Applications

In a second assay, a mixed microbial population composed of *P. aeruginosa, Burkholderia cepacia* and *E. coli* were spiked and analyzed with the procedure described in "Combination of Total Viable Count and Specific Detection of P. aeruginosa." Results are presented in the figure 11. After 9 hours growth at 35 °C, 24 CFUs were detected after the TVC procedure

This demonstrates that the system is able to make TVC and specific detection even in a

**2D view 3D view A** 

**B** 

and 8 CFUs were detected using the *P. aeruginosa* specific detection procedure.

**Rapid microbiology count 24 CFUs Specific detection count 8 CFUs** 

**contaminants in the mix 29% Incubation Time 9 h Overall procedure 11 h 30** 

microbial contamination rapidly in a variety of filterable samples.

Fig. 11. Specific detection of *P. aeruginosa* after TVC on the same membrane using a mixed population of *P. aeruginosa* ATCC 9027, B. cepacia ATCC 25416 and E.coli ATCC 25922

The different studies presented here show how versatile is the use of Bioluminescence for microorganisms detection. We demonstrate here that it offers a high sensitivity to detect

mixed population of microorganisms.

**Milliflex Rapid Microbiology Detection of TVC (A) and**  *P.aeruginosa* **specific detection (B) in a mixed population composed of** *P.aeruginosa***,** *B.cepacia* **&**  *E. coli*

[TSA, 32.5 ± 2.5°C]

**Percentage of** *P.aeruginosa*

incubated on TSA at 32.5°C+/-2.5°C.

**4. Conclusion** 

Authors would like to thanks colleagues from Merck-Millipore Application group, Development group and Predevelopment - Technology – Collaboration for their technical collaboration. The research described in this paper was carried out at the Merck-Millipore R&D laboratory (Molsheim, France).
