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**6** 

*UK* 

**Development of a pH-Tolerant** 

**Thermostable** *Photinus pyralis*

Amit Jathoul1, Erica Law2, Olga Gandelman3,\*, Martin Pule1, Laurence Tisi3 and Jim Murray4

> *1Cancer Institute, University College London, 2Illumina Inc., Chesterford Research Park, 3Lumora Ltd., Cambridgeshire Business Park, 4School of Biosciences, Cardiff University*

**Luciferase for Brighter** *In Vivo* **Imaging** 

Firefly luciferase (Fluc) catalyzes a bioluminescent reaction using the substrates ATP and beetle luciferin in the presence of molecular oxygen (Fig. 1A). Because of its use of ATP and the simplicity of the single-enzyme system, firefly luciferase is widely used in numerous applications, notably those involving detection of living organisms, gene expression or

**A** 

**B C**  Fig. 1. Bioluminescent reaction of Fluc (A) and chemical structures of luciferin (LH2) (B) and

Fluc has found intensive application in small animal *in vivo* bioluminescence imaging (BLI) in which the activity or state of labelled proteins, cells, tissues and organs may be localised

**1. Introduction** 

 \*

Corresponding Author

amplification in both *in vivo* and *in vitro* systems.

aminoluciferin (ALH2) (C) eliciting intense bioluminescence.

