**3.2 Effects of AV-6-93 and diflurone on rat liver mitochondrial bioenergetics**

AV-6-93 and diflurone (up to 100 μM) were studied for their effects on mitochondrial bioenergetics by evaluating several mitochondrial respiratory chain parameters (state 2, state 3, state 4, FCCP-stimulated respiration, RCR, ADP/O ratio, ∆, and phosphorylation rate) using glutamate/malate as the respiratory substrate.

The effects of AV-6-93 on glutamate/malate-supported respiratory rates (state 2, state 3, state 4 and FCCP-stimulated respiration), respiratory indices RCR and ADP/O of rat liver mitochondria were almost non-existent and insignificant at concentrations of up to 100 µM (Table 1), indicating that the compounds did not significantly affect mitochondrial bioenergetics.

These results are demonstrated in Table 2, where AV-6-93 and diflurone, at concentrations of up to 100 µM, did not significantly affect either the ∆ induced by glutamate/malatedependent respiration or the phosphorylation time.

As for glutamate/malate-supported respiration, the effects of AV-6-93 and diflurone on succinate-supported respiratory rates (state 2, state 3, state 4 and FCCP**)** and respiratory indices RCR and ADP/O of rat liver mitochondria were not significantly affected (results not shown), further supporting the finding that these compounds did not affect mitochondrial bioenergetics.
