**3. Modulatory effect of P2X receptors on synaptic transmission**

Following the discovery of purinergic neurotransmission in 1972 in non-adrenergic, noncholinergic inhibitory nerves in guinea-pig taenia coli, ATP was identified as a cotransmitter in both sympathetic and parasympathetic peripheral nerves and latter also in the central nervous systems (Burnstock, 2011). Of all tissues investigated, the mammalian brain has the highest levels of purines and the greatest variety of the ATP-binding P2XRs (Buell et al., 1996; Collo et al., 1996; Seguela et al., 1996). Both neurons and glial cells release ATP and express P2XRs (Fields and Stevens, 2000; Raivich, 2005; Inoue et al., 2007) and it is common that several subtypes of P2XRs are expressed in the plasma membrane of one cell (Abbracchio et al., 2009). Neurons release ATP by exocytosis together with other neurotransmitters, such as GABA, glycine, glutamate and noradrenaline (Jo and Schlichter, 1999; Robertson et al., 2001; Sokolova et al., 2001; Jo and Role, 2002; Day et al., 1993). Glia have been shown to release ATP in response to mechanical and electrical stimulation (Newman, 2003; Burnstock, 2004), although the precise mechanisms have not been identified. The most frequent receptor forms in the brain are P2X2, P2X4, and P2X6 as well as heteromers composed of P2X2+X6, P2X4+X6, and perhaps P2X1+X4 receptors (Buell et al., 1996; Collo et al., 1996). Activation of P2X receptors by extracellular ATP acts mainly as a short-term signal but has also several long-term effects. The short-term effects involve fast synaptic transmission mediated by ATP in both the peripheral (Evans et al., 1992) and central nervous systems (Edwards et al., 1992), modulation of neuronal excitability (Khakh and Henderson, 1998) and long-term potentiation (Sim et al., 2006). Long-term (trophic) role comprise cell proliferation, differentiation and death, growth of axons during development and regeneration (Heine et al., 2006; Burnstock, 2011). Like other transmitters, ATP can

Facilitation of Neurotransmitter and Hormone Release by P2X Purinergic Receptors 67

acute hypothalamic explants revealed that it was due to depolarization of membrane potential following exposure to ATP (Hiruma and Bourque, 1995). In voltage-clamped SON neurons of rat brain slices, application of ATP (10–100 M) stimulated inward currents that were potentiated by acidic pH and attenuated by PPADS suggesting the involvement of P2X2R (Vavra et al., 2011). Similar ATP-evoked currents were recorded from neuronal somata of acutely dissociated SON neurons (Shibuya et al., 1999) and isolated terminals of

There are also evidences for the contribution of P2X4Rs, which are particularly prevalent in the central nervous system and hypothalamus (Illes and Ribeiro, 2004; Collo et al., 1996; Stojilkovic, 2009; Jo et al., 2011). Ivermectin, a receptor-specific allosteric modulator (Khakh et al., 1999) caused more than 2-fold augmentation of the maximum amplitude of ATPevoked current and prolonged the ATP-stimulated calcium responses in SON neurons of slices (Vavra et al., 2011). A similar potentiating effect of ivermectin has been shown previously in neurons from the somato-sensory cortex (Lalo et al., 2007) and pituitary lactorophs that endogenously express homomeric or heteromeric P2X4Rs (Zemkova et al., 2010). The novel inhibitor of recombinant P2X4Rs, 5BDBD (Wu et al., 2011), failed to affect ATP-induced responses in neurons (Vavra et al., 2011), however, it may be ineffective on heteromeric P2X4Rs which prevail in the brain. The presence of the functional P2X2R and P2X4Rs on SON neurons was further corroborated by the qRT-PCR experiments, which demonstrated significant expression of mRNA transcripts for these receptors in SON tissue (Vavra et al., 2011). Significant level of mRNA for P2X7R was also observed but P2X1, P2X3, P2X5 and P2X6 mRNA expression levels were minor. The high mRNA level for P2X7 can be ascribed to P2X receptors expressed in non-neuronal cells, such as microglia (Collo et al., 1997), because the currents of SON neurons were not affected by BzATP, but non-neuronal SON cells in slices responded with an increase in intracellular

Fig. 2. Expression and function of P2X receptors in hypothalamus.

the hypothalamic neurohypophysial system (Knott et al., 2005).

calcium (Vavra et al., 2011).

mediate both reciprocal interactions between neurons and glia, and intercellular communication in astrocytic networks (Guthrie et al., 1999; Newman, 2003; Fields and Burnstock, 2006; Burnstock, 2007).

#### **3.1 Expression of P2X receptor subtypes in hypothalamus**

Purinergic P2X receptors are expressed throughout the hypothalamus where ATP, coreleased with neuropeptides, appears to be involved in the regulation of hormone secretion (Troadec et al., 1998; Kapoor and Sladek, 2000) and control of specific autonomic functions including the central mechanism of body temperature regulation (Gourine et al., 2002), for example. P2X2, P2X4 and P2X6, but not P2X5 receptor mRNAs have been identified in the rat supraoptic nucleus (SON), ventromedial nucleus, paraventricular nucleus, arcuate nucleus, suprachiasmatic nucleus, and several other hypothalamic areas using in situ hybridization; the P2X1, P2X3 and P2X7 receptors were not tested in this study (Collo et al., 1996). Another PCR analysis revealed that P2X2, P2X3, P2X4, P2X6, and P2X7 receptor mRNAs are expressed in the rat SON neurons; P2X1 and P2X5 mRNAs were not found (Shibuya et al., 1999). In the preoptic area of the rhesus monkey, the P2X2, P2X4 and P2X7 receptor mRNAs, but not P2X1, P2X3 and P2X5 receptor mRNAs, were identified; subunit P2X6 was not examined (Terasawa et al., 2005). Immunohistochemistry shows local differences in P2XR protein distribution in the rat hypothalamus. For example, the P2X2R immunoreactive neurons and nerve fibers have been found to be localized in the paraventricular nucleus, arcuate nucleus, retrochiasmatic area, periventricular nucleus, the ventral part of tuber cinereum area, supraoptic, circular, and ventral tuberomammillary nuclei, organum vasculosum, and median eminence (Xiang et al., 1998; Yao et al., 2003), but are absent in the ventromedial nucleus (Vulchanova et al., 1996; Xiang et al., 1998). The magnocellular neurons from paraventricular and supraoptic nuclei synthesize vasopressin and oxytocin and transport them to the axonal terminals in the posterior pituitary where they are secreted into the general circulation. Double-labeling fluorescence immunohistochemistry has shown that both vasopressin- and oxytocin-containing neurons in the SON express P2X2, P2X4 and P2X5 receptor proteins (Guo et al., 2009). P2X2Rpositive immunoreactivity has been found both on SON somata and nerve fibers (Yao et al., 2003; Vulchanova et al., 1996; Xiang et al., 1998; Shibuya et al., 1999; Loesch and Burnstock, 2001). Our quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis showed the expression of mRNAs of all P2XR subunits and several P2YRs in SONcontaining tissue; the expression of mRNAs for ionotropic P2X2R > P2X7R > P2X4R subunits was the most significant (Fig.2) (Vavra et al., 2011). Thus hypothalamus has a capacity to express both P2XR and P2YR.

Functional studies showed that application of ATP caused an increase in Ca2+ in cultured hypothalamic neurons (Chen et al., 1994), acutely isolated SON neurons and glial cells (Shibuya et al., 1999), SON neurons in hypothalamo-neurohypophyseal system explants (Song et al., 2007) and rat brain slices (Vavra et al., 2011). Application of ATP also stimulates the release of vasopressin and oxytocin in perfused explants of the hypothalamoneurohypophyseal system (Gomes et al., 2009) and isolated neurohypophysial nerve terminals of the rat (Troadec et al., 1998). These ATP-induced effects were attenuated by the P2X receptor antagonists suramin and PPADS indicating that P2X2Rs or P2X3Rs play a role. Extracellular recordings showed that ATP application increases excitability of SON neurons in the hypothalamus (Day et al., 1993) and intracellular recordings from SON neurons in

mediate both reciprocal interactions between neurons and glia, and intercellular communication in astrocytic networks (Guthrie et al., 1999; Newman, 2003; Fields and

Purinergic P2X receptors are expressed throughout the hypothalamus where ATP, coreleased with neuropeptides, appears to be involved in the regulation of hormone secretion (Troadec et al., 1998; Kapoor and Sladek, 2000) and control of specific autonomic functions including the central mechanism of body temperature regulation (Gourine et al., 2002), for example. P2X2, P2X4 and P2X6, but not P2X5 receptor mRNAs have been identified in the rat supraoptic nucleus (SON), ventromedial nucleus, paraventricular nucleus, arcuate nucleus, suprachiasmatic nucleus, and several other hypothalamic areas using in situ hybridization; the P2X1, P2X3 and P2X7 receptors were not tested in this study (Collo et al., 1996). Another PCR analysis revealed that P2X2, P2X3, P2X4, P2X6, and P2X7 receptor mRNAs are expressed in the rat SON neurons; P2X1 and P2X5 mRNAs were not found (Shibuya et al., 1999). In the preoptic area of the rhesus monkey, the P2X2, P2X4 and P2X7 receptor mRNAs, but not P2X1, P2X3 and P2X5 receptor mRNAs, were identified; subunit P2X6 was not examined (Terasawa et al., 2005). Immunohistochemistry shows local differences in P2XR protein distribution in the rat hypothalamus. For example, the P2X2R immunoreactive neurons and nerve fibers have been found to be localized in the paraventricular nucleus, arcuate nucleus, retrochiasmatic area, periventricular nucleus, the ventral part of tuber cinereum area, supraoptic, circular, and ventral tuberomammillary nuclei, organum vasculosum, and median eminence (Xiang et al., 1998; Yao et al., 2003), but are absent in the ventromedial nucleus (Vulchanova et al., 1996; Xiang et al., 1998). The magnocellular neurons from paraventricular and supraoptic nuclei synthesize vasopressin and oxytocin and transport them to the axonal terminals in the posterior pituitary where they are secreted into the general circulation. Double-labeling fluorescence immunohistochemistry has shown that both vasopressin- and oxytocin-containing neurons in the SON express P2X2, P2X4 and P2X5 receptor proteins (Guo et al., 2009). P2X2Rpositive immunoreactivity has been found both on SON somata and nerve fibers (Yao et al., 2003; Vulchanova et al., 1996; Xiang et al., 1998; Shibuya et al., 1999; Loesch and Burnstock, 2001). Our quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis showed the expression of mRNAs of all P2XR subunits and several P2YRs in SONcontaining tissue; the expression of mRNAs for ionotropic P2X2R > P2X7R > P2X4R subunits was the most significant (Fig.2) (Vavra et al., 2011). Thus hypothalamus has a

Functional studies showed that application of ATP caused an increase in Ca2+ in cultured hypothalamic neurons (Chen et al., 1994), acutely isolated SON neurons and glial cells (Shibuya et al., 1999), SON neurons in hypothalamo-neurohypophyseal system explants (Song et al., 2007) and rat brain slices (Vavra et al., 2011). Application of ATP also stimulates the release of vasopressin and oxytocin in perfused explants of the hypothalamoneurohypophyseal system (Gomes et al., 2009) and isolated neurohypophysial nerve terminals of the rat (Troadec et al., 1998). These ATP-induced effects were attenuated by the P2X receptor antagonists suramin and PPADS indicating that P2X2Rs or P2X3Rs play a role. Extracellular recordings showed that ATP application increases excitability of SON neurons in the hypothalamus (Day et al., 1993) and intracellular recordings from SON neurons in

Burnstock, 2006; Burnstock, 2007).

capacity to express both P2XR and P2YR.

**3.1 Expression of P2X receptor subtypes in hypothalamus** 

Fig. 2. Expression and function of P2X receptors in hypothalamus.

acute hypothalamic explants revealed that it was due to depolarization of membrane potential following exposure to ATP (Hiruma and Bourque, 1995). In voltage-clamped SON neurons of rat brain slices, application of ATP (10–100 M) stimulated inward currents that were potentiated by acidic pH and attenuated by PPADS suggesting the involvement of P2X2R (Vavra et al., 2011). Similar ATP-evoked currents were recorded from neuronal somata of acutely dissociated SON neurons (Shibuya et al., 1999) and isolated terminals of the hypothalamic neurohypophysial system (Knott et al., 2005).

There are also evidences for the contribution of P2X4Rs, which are particularly prevalent in the central nervous system and hypothalamus (Illes and Ribeiro, 2004; Collo et al., 1996; Stojilkovic, 2009; Jo et al., 2011). Ivermectin, a receptor-specific allosteric modulator (Khakh et al., 1999) caused more than 2-fold augmentation of the maximum amplitude of ATPevoked current and prolonged the ATP-stimulated calcium responses in SON neurons of slices (Vavra et al., 2011). A similar potentiating effect of ivermectin has been shown previously in neurons from the somato-sensory cortex (Lalo et al., 2007) and pituitary lactorophs that endogenously express homomeric or heteromeric P2X4Rs (Zemkova et al., 2010). The novel inhibitor of recombinant P2X4Rs, 5BDBD (Wu et al., 2011), failed to affect ATP-induced responses in neurons (Vavra et al., 2011), however, it may be ineffective on heteromeric P2X4Rs which prevail in the brain. The presence of the functional P2X2R and P2X4Rs on SON neurons was further corroborated by the qRT-PCR experiments, which demonstrated significant expression of mRNA transcripts for these receptors in SON tissue (Vavra et al., 2011). Significant level of mRNA for P2X7R was also observed but P2X1, P2X3, P2X5 and P2X6 mRNA expression levels were minor. The high mRNA level for P2X7 can be ascribed to P2X receptors expressed in non-neuronal cells, such as microglia (Collo et al., 1997), because the currents of SON neurons were not affected by BzATP, but non-neuronal SON cells in slices responded with an increase in intracellular calcium (Vavra et al., 2011).

Facilitation of Neurotransmitter and Hormone Release by P2X Purinergic Receptors 69

(Valera et al., 1994). Another role of the P2X1R in the brain thus might be in the smooth muscle of small arteries and arterioles, allowing the control of blood flow and pressure.

Fig. 3. Presynaptic effect of ATP on spontaneous glutamate and GABA release.

rat brain slices. (\*) P < 0.01. For details see: (Vavra et al., 2011).

A, ATP (100 μM) induced inward current and increase in the frequency of spontaneous GABAergic inhibitory postsynaptic currents (sIPSC). B, ATP induced increases in the frequency of spontaneous glutamatergic excitatory postsynaptic currents (sEPSC). Traces in an expanded time scale show spontaneous synaptic currents before (Control) and after ATP application (ATP). Glutamatergic and GABAergic spontaneous postsynaptic currents differ in their amplitudes and duration. Experiments were performed on SON neurons voltage clamped at -60 mV. C,D, Summary histograms showing the effect of ATP on the frequency (C) and amplitude (D) of sEPSCs and sIPSCs. Analysis was performed on SON neurons in

Numerous studies have shown that P2X2Rs act presynaptically to increase glutamate in several brain areas. Experiments with knockout mice showed that Ca2+ entry through presynaptic P2X2Rs increases the frequency of spontaneous AMPA receptor-mediated glutamatergic currents in GABAergic hippocampal interneurons (Khakh et al., 2003). Furthermore, inhibiting P2XRs by application of PPADS has been shown to abolish the glutamate-dependent postsynaptic currents evoked by focal application of ATP in dorsal horn neurons (Li et al., 1998 Nakatsuka and Gu, 2001). PPADS-sensitive P2X2Rs have been shown in glutamatergic terminals of neurons in trigeminal mesencephalic motor nucleus (Khakh and Henderson, 1998), the nucleus tractus solitari (Shigetomi and Kato, 2004) and the area postrema (Kodama et al., 2007). The presynaptic P2X2Rs have been shown to

ATP-evoked inward current mediated by P2X2 or P2X4 receptors, as observed in SON neurons, likely represents a particular example of a more general function of P2XRs: to make voltage-threshold more positive and to drive neurons to fire action potentials. We suppose the functional role of somatic P2XRs in the brain consists in controlling excitability close to the nominal resting potential of the neuron cell body, near − 60 mV (Vavra et al., 2011).

A, The presence of seven P2XRs (black columns) and three P2YRs (gray columns) mRNA transcripts in hypothalamic tissues from 16 day-old rats. Quantitative RT-PCR analysis shows significant expressions of ionotropic P2X2, P2X4 and P2X7 receptor mRNAs and moderate levels of mRNA for P2X3, P2Y1 and P2Y2 receptors. The mRNA levels of P2 receptor genes were related to the expression of GAPDH as a housekeeping gene /endogenous control. B,C Example recordings of action potentials from the SON neuron in slices before, during and after the application (bar) of 100 µM ATP (B) and 100 µM ADP (D). ATP induced depolarization and an increase in frequency of action potentials. Neither effect was observed after the application of ADP which had a tendency to decrease the frequency of action potentials. All neurons had resting membrane potential (Vm) lower than -50 mV when measured in current clamp mode. For details see: (Vavra et al., 2011).

#### **3.2 Modulation of glutamate and GABA release by presynaptic P2XRs**

ATP is viewed as a neurotransmitter in both the peripheral (Evans et al., 1992) and the central nervous system (Pankratov et al., 1998). It has been suggested that endogenously released ATP acts at postsynaptic P2XRs to mediate synaptic currents in the hippocampus (Edwards et al., 1992). This classical view, however, is challenged by a series of recent studies that showed that ATP acts rather as a modulator that stimulates the release of other neurotransmitters (Li et al., 1998; Sperlagh et al., 2002; Watano et al., 2004; Rodrigues et al., 2005; Kodama et al., 2007; Sperlagh et al., 2007; Donato et al., 2008). In the SON neurons, for instance, all spontaneous synaptic currents were inhibited by blockers of glutamate and GABAA receptors and application of ATP stimulated about 2300 % increase in frequency of both spontaneous inhibitory and excitatory postsynaptic currents without changes in their amplitude (Fig.3) (Vavra et al., 2011). This finding suggests that ATP does not act as a spontaneously released neurotransmitter, but as a modulator that stimulates the release of GABA or glutamate (or both) by activating purinergic receptors in the nerve terminals (Vavra et al., 2011). These experiments were performed in the presence of tetrodotoxin, which inhibits action potentials, thus P2XRs are implicated in presynaptic function by stimulating Ca2+ influx into the nerve terminals. Known presynaptic P2XR subtypes with a role in neurotransmission include P2X1, P2X2, P2X3 and possibly P2X4 and P2X7 receptors.

P2X1Rs have been localized and implicated in the function of sympathetic ganglion neurons (Calvert and Evans, 2004), cerebellum (Hervas et al., 2003) and spinal cord dorsal horn sensory neurons (Nakatsuka et al., 2003). Presynaptic P2X1R have been found to mediate agonist-evoked release of tritium-labeled glutamate from purified nerve terminals of the rat hippocampus (Rodrigues et al., 2005). Facilitation of both glutamate and GABA release mediated by presynaptic P2X1R was observed in the rat medial nucleus of the trapezoid body (Watano et al., 2004). Activation of presynaptic P2X1R elicits noradrenaline release from central catecholaminergic terminals in superfused rat hippocampal slices (Papp et al., 2004). These receptors are present in high concentrations on some smooth muscle cells

ATP-evoked inward current mediated by P2X2 or P2X4 receptors, as observed in SON neurons, likely represents a particular example of a more general function of P2XRs: to make voltage-threshold more positive and to drive neurons to fire action potentials. We suppose the functional role of somatic P2XRs in the brain consists in controlling excitability close to the

A, The presence of seven P2XRs (black columns) and three P2YRs (gray columns) mRNA transcripts in hypothalamic tissues from 16 day-old rats. Quantitative RT-PCR analysis shows significant expressions of ionotropic P2X2, P2X4 and P2X7 receptor mRNAs and moderate levels of mRNA for P2X3, P2Y1 and P2Y2 receptors. The mRNA levels of P2 receptor genes were related to the expression of GAPDH as a housekeeping gene /endogenous control. B,C Example recordings of action potentials from the SON neuron in slices before, during and after the application (bar) of 100 µM ATP (B) and 100 µM ADP (D). ATP induced depolarization and an increase in frequency of action potentials. Neither effect was observed after the application of ADP which had a tendency to decrease the frequency of action potentials. All neurons had resting membrane potential (Vm) lower than -50 mV

ATP is viewed as a neurotransmitter in both the peripheral (Evans et al., 1992) and the central nervous system (Pankratov et al., 1998). It has been suggested that endogenously released ATP acts at postsynaptic P2XRs to mediate synaptic currents in the hippocampus (Edwards et al., 1992). This classical view, however, is challenged by a series of recent studies that showed that ATP acts rather as a modulator that stimulates the release of other neurotransmitters (Li et al., 1998; Sperlagh et al., 2002; Watano et al., 2004; Rodrigues et al., 2005; Kodama et al., 2007; Sperlagh et al., 2007; Donato et al., 2008). In the SON neurons, for instance, all spontaneous synaptic currents were inhibited by blockers of glutamate and GABAA receptors and application of ATP stimulated about 2300 % increase in frequency of both spontaneous inhibitory and excitatory postsynaptic currents without changes in their amplitude (Fig.3) (Vavra et al., 2011). This finding suggests that ATP does not act as a spontaneously released neurotransmitter, but as a modulator that stimulates the release of GABA or glutamate (or both) by activating purinergic receptors in the nerve terminals (Vavra et al., 2011). These experiments were performed in the presence of tetrodotoxin, which inhibits action potentials, thus P2XRs are implicated in presynaptic function by stimulating Ca2+ influx into the nerve terminals. Known presynaptic P2XR subtypes with a role in neurotransmission include P2X1, P2X2, P2X3 and possibly P2X4 and P2X7 receptors. P2X1Rs have been localized and implicated in the function of sympathetic ganglion neurons (Calvert and Evans, 2004), cerebellum (Hervas et al., 2003) and spinal cord dorsal horn sensory neurons (Nakatsuka et al., 2003). Presynaptic P2X1R have been found to mediate agonist-evoked release of tritium-labeled glutamate from purified nerve terminals of the rat hippocampus (Rodrigues et al., 2005). Facilitation of both glutamate and GABA release mediated by presynaptic P2X1R was observed in the rat medial nucleus of the trapezoid body (Watano et al., 2004). Activation of presynaptic P2X1R elicits noradrenaline release from central catecholaminergic terminals in superfused rat hippocampal slices (Papp et al., 2004). These receptors are present in high concentrations on some smooth muscle cells

nominal resting potential of the neuron cell body, near − 60 mV (Vavra et al., 2011).

when measured in current clamp mode. For details see: (Vavra et al., 2011).

**3.2 Modulation of glutamate and GABA release by presynaptic P2XRs** 

(Valera et al., 1994). Another role of the P2X1R in the brain thus might be in the smooth muscle of small arteries and arterioles, allowing the control of blood flow and pressure.

Fig. 3. Presynaptic effect of ATP on spontaneous glutamate and GABA release.

A, ATP (100 μM) induced inward current and increase in the frequency of spontaneous GABAergic inhibitory postsynaptic currents (sIPSC). B, ATP induced increases in the frequency of spontaneous glutamatergic excitatory postsynaptic currents (sEPSC). Traces in an expanded time scale show spontaneous synaptic currents before (Control) and after ATP application (ATP). Glutamatergic and GABAergic spontaneous postsynaptic currents differ in their amplitudes and duration. Experiments were performed on SON neurons voltage clamped at -60 mV. C,D, Summary histograms showing the effect of ATP on the frequency (C) and amplitude (D) of sEPSCs and sIPSCs. Analysis was performed on SON neurons in rat brain slices. (\*) P < 0.01. For details see: (Vavra et al., 2011).

Numerous studies have shown that P2X2Rs act presynaptically to increase glutamate in several brain areas. Experiments with knockout mice showed that Ca2+ entry through presynaptic P2X2Rs increases the frequency of spontaneous AMPA receptor-mediated glutamatergic currents in GABAergic hippocampal interneurons (Khakh et al., 2003). Furthermore, inhibiting P2XRs by application of PPADS has been shown to abolish the glutamate-dependent postsynaptic currents evoked by focal application of ATP in dorsal horn neurons (Li et al., 1998 Nakatsuka and Gu, 2001). PPADS-sensitive P2X2Rs have been shown in glutamatergic terminals of neurons in trigeminal mesencephalic motor nucleus (Khakh and Henderson, 1998), the nucleus tractus solitari (Shigetomi and Kato, 2004) and the area postrema (Kodama et al., 2007). The presynaptic P2X2Rs have been shown to

Facilitation of Neurotransmitter and Hormone Release by P2X Purinergic Receptors 71

In the rat suprachiasmatic nuclei, ATP content and extracellular ATP levels (Yamazaki et al., 1994; Womac et al., 2009) negatively correlate with electrical activity and arginine vasopressin (AVP) secretion rhythm: the frequency of action potentials and the level of AVP is high during the day and low during the night, but ATP release peaks during the night. This might indicate that ATP is primarily stored and released from distinct pool of vesicles and/or that ATP and AVP are released from different cells. How ATP release from hypothalamic cells is regulated and what is the reason for local differences in distribution of

Depolarization and Ca2+ influx stimulated by extracellular ATP has numerous functions also in endocrine cells. These involve stimulation of luteinizing hormone, prolactin, oxytocin and vasopressin hormone secretion by pituitary gland (Kapoor and Sladek, 2000; Stojilkovic, 2009; Stojilkovic et al., 2010b). The anterior pituitary is a heterogeneous gland with multiple cell types that secrete six major peptide hormones necessary for reproduction, lactation, growth, development, metabolic homeostasis, and the response to stress: FSH and LHproducing gonadotrophs, prolactin (PRL)-producing lactotrophs, GH-producing somatotrophs, TSH-producing thyrotrophs, and ACTH-producing corticotrophs. This lobe also contains the non-hormone-producing folliculostellate cells, which are glia-like cells, and endothelial cells that line the capillaries. Pituitary hormone secretion is under control of hypothalamic neurohormones and is also modulated by extracellular ATP (Chen et al., 1995) that is released by the anterior pituitary itself in a regulated manner, or coreleased with hypothalamic peptides (Tomic et al., 1996; Lazarowski et al., 2000; Stojilkovic and Koshimizu, 2001; He et al., 2005). The mRNA transcripts for several P2X subunits (P2X2R, P2X3R, P2X4R, and P2X7R) were identified in anterior pituitary cells from neonatal (Zemkova *et al.*, 2006) and adult rats (Koshimizu et al., 2000a; Koshimizu et al., 2000b; Stojilkovic and Koshimizu, 2001), including two spliced forms of the P2X2R subunit. Experiments with the plasma membrane-targeted luciferase expressed in HEK cells or ACN neuroblastoma cells indicated that endogenous extracellular ATP concentrations are in the range of 100-200 µM, which is more than sufficient to activate all types of P2X receptors (Pellegatti et al., 2005). *In vivo*, the ATP action on gonadotroph functions could be controlled by ectonucleotidases 1-3, which are expressed in pituitary cells (He et al., 2005) and provide

P2X receptor subtypes is still being elucidated.

**4. Potentiation of hormone secretion in pituitary gland** 

an effective pathway for the control of extracellular ATP concentrations.

details see: (Stojilkovic et al., 2010b).

Detection and localization of mRNA transcripts for P2X2, P2X3 and P2X4 receptors in pituitary gland by in situ hybridization. *PP,* posterior pituitary, *AP,* anterior pituitary. For

Most anterior pituitary cells express functional P2X receptors (Fig.4) (Carew et al., 1994; Villalobos et al., 1997; Koshimizu et al., 2000a). The P2X2Rs were identified in pituitary gonadotrophs (Tomic et al., 1996; Koshimizu et al., 2000b; Zemkova et al., 2006) and somatotrophs (Koshimizu et al., 2000a). Lactotrophs express functional P2X4Rs (Carew et al., 1994; He et al., 2003; Zemkova et al., 2010) as well as P2X7R subtypes (Chung et al., 2000; He et al., 2003). Corticotrophs seem not to respond to extracellular ATP directly by stimulation of any P2X receptor (Zhao et al., 2006), and identification of P2X receptors in remaining anterior pituitary cell type, thyrotrophs, has not yet been done. Functional studies showed that ATP application induces inward slowly desensitizig current and increases

underlie increase in GABA release in a subset of GABAergic interneurons in the spinal cord (Hugel and Schlichter, 2000) and in Purkinje cells in rat cerebellar slices (Donato et al., 2008). In SON neurons, the ATP-induced increases in the frequency of spontaneous excitatory and inhibitory postsynaptic currents were also inhibited by PPADS and potentiated by pH 6.5, indicating the involvement of presynaptic P2X2Rs in the release of both neurotransmitters (Vavra et al., 2011).

The P2X3Rs are almost exclusively expressed in dorsal root ganglion (DRG) neuronal processes that are involved in acute pain (Vulchanova et al., 1997; Dunn et al., 2001; North, 2004). Immunocytochemistry for P2X3 subunits revealed that these receptors are expressed in small- and medium-sized neurons but are absent from large diameter neurons (Vulchanova et al., 1997; Vulchanova et al., 1998; Novakovic et al., 1999). Stimulation of presynaptic P2XRs in DRG neuronal processes by ATP application induced glutamate release that activated postsynaptic glutamatergic receptors expressed in the dorsal horn neurons (Gu and MacDermott, 1997) and that was inhibited by PPADS. Presynaptic P2X3R have been also found in other brain areas, including purified nerve terminals of the rat hippocampus (Rodrigues et al., 2005), in cranial afferent neurons in nodose ganglia (Kato and Shigetomi, 2001; Jin et al., 2004), in the midbrain periaqueductal gray (Xing et al., 2008), in the rat medial nucleus of the trapezoid body (Watano et al., 2004). ATP-stimulated GABA release from rat midbrain terminals are mediated through the activation of P2X receptors with an abundance of P2X3 subunits (Gomez-Villafuertes et al., 2001). Activation of presynaptic P2X3R receptors contributes to noradrenaline release in superfused rat hippocampal slices (Papp et al., 2004).

Evidence for the presence of P2X4Rs in the nerve terminals is less obvious because selective antagonists are lacking. We showed that the ATP-induced effect on frequency of spontaneous inhibitory postsynaptic currents in the SON declined during prolonged ATP application, which indicates the involvement of a desensitizing P2X4R (Vavra et al., 2011).

BzATP has been reported to enhance release of glutamate by acting at P2X7Rs in purified rat neocortex synaptosomes (Patti et al., 2006). Possible presynaptic mechanisms underlying enhancement of excitatory transmitter release by P2X7R has been also suggested for hypoglossal motoneurons in brainstem slices (Ireland et al., 2004). However, neuronal origin of P2X7R is still uncertain. The P2X7Rs are expressed on satellite glial cells enwrapping the peripheral neurons (e.g., DRG neurons) and on microglia, astrocytes and oligodendrocytes in the brain. After repeated or prolonged exposure to ATP, P2X7R are able to form pores permeable to small molecules, and could mediate release of cytokines from satellite glial and microglial cells (Ferrari et al., 1997b; Ferrari et al., 1997a; Ferrari et al., 2006; Matute et al., 2007) or release of neurotransmitters from astrocytes. Several reports have shown that the P2X7R, and not other P2XRs, is responsible for IL-1β release from activated myeloid cells (Mehta et al., 2001; Le Feuvre et al., 2002). LPS-primed macrophages from transgenic mice lacking P2X7 receptors fail to release IL-1β in response to ATP, although synthesis of pro-IL-1β and caspase-1 is unaltered (Solle et al., 2001; Labasi et al., 2002). Activation of P2X7Rs has been reported to elicit the release of glutamate (Duan et al., 2003), GABA (Wang et al., 2002), 2 arachidonoylglycerol (Walter et al., 2004), and purines (Ballerini et al., 1996) from cultured astrocytes. In neurological diseases or injuries extracellular ATP may activate P2X7Rs further enhancing purine release, with important pathophysiological consequences (Ballerini et al., 1996). In paraventricular nucleus of hypothalamus, P2X7 receptor activation results in insertion of AMPA receptors into postsynaptic neuronal membrane (Gordon et al., 2009).

underlie increase in GABA release in a subset of GABAergic interneurons in the spinal cord (Hugel and Schlichter, 2000) and in Purkinje cells in rat cerebellar slices (Donato et al., 2008). In SON neurons, the ATP-induced increases in the frequency of spontaneous excitatory and inhibitory postsynaptic currents were also inhibited by PPADS and potentiated by pH 6.5, indicating the involvement of presynaptic P2X2Rs in the release of both neurotransmitters

The P2X3Rs are almost exclusively expressed in dorsal root ganglion (DRG) neuronal processes that are involved in acute pain (Vulchanova et al., 1997; Dunn et al., 2001; North, 2004). Immunocytochemistry for P2X3 subunits revealed that these receptors are expressed in small- and medium-sized neurons but are absent from large diameter neurons (Vulchanova et al., 1997; Vulchanova et al., 1998; Novakovic et al., 1999). Stimulation of presynaptic P2XRs in DRG neuronal processes by ATP application induced glutamate release that activated postsynaptic glutamatergic receptors expressed in the dorsal horn neurons (Gu and MacDermott, 1997) and that was inhibited by PPADS. Presynaptic P2X3R have been also found in other brain areas, including purified nerve terminals of the rat hippocampus (Rodrigues et al., 2005), in cranial afferent neurons in nodose ganglia (Kato and Shigetomi, 2001; Jin et al., 2004), in the midbrain periaqueductal gray (Xing et al., 2008), in the rat medial nucleus of the trapezoid body (Watano et al., 2004). ATP-stimulated GABA release from rat midbrain terminals are mediated through the activation of P2X receptors with an abundance of P2X3 subunits (Gomez-Villafuertes et al., 2001). Activation of presynaptic P2X3R receptors contributes to noradrenaline release in superfused rat

Evidence for the presence of P2X4Rs in the nerve terminals is less obvious because selective antagonists are lacking. We showed that the ATP-induced effect on frequency of spontaneous inhibitory postsynaptic currents in the SON declined during prolonged ATP application, which indicates the involvement of a desensitizing P2X4R (Vavra et al., 2011). BzATP has been reported to enhance release of glutamate by acting at P2X7Rs in purified rat neocortex synaptosomes (Patti et al., 2006). Possible presynaptic mechanisms underlying enhancement of excitatory transmitter release by P2X7R has been also suggested for hypoglossal motoneurons in brainstem slices (Ireland et al., 2004). However, neuronal origin of P2X7R is still uncertain. The P2X7Rs are expressed on satellite glial cells enwrapping the peripheral neurons (e.g., DRG neurons) and on microglia, astrocytes and oligodendrocytes in the brain. After repeated or prolonged exposure to ATP, P2X7R are able to form pores permeable to small molecules, and could mediate release of cytokines from satellite glial and microglial cells (Ferrari et al., 1997b; Ferrari et al., 1997a; Ferrari et al., 2006; Matute et al., 2007) or release of neurotransmitters from astrocytes. Several reports have shown that the P2X7R, and not other P2XRs, is responsible for IL-1β release from activated myeloid cells (Mehta et al., 2001; Le Feuvre et al., 2002). LPS-primed macrophages from transgenic mice lacking P2X7 receptors fail to release IL-1β in response to ATP, although synthesis of pro-IL-1β and caspase-1 is unaltered (Solle et al., 2001; Labasi et al., 2002). Activation of P2X7Rs has been reported to elicit the release of glutamate (Duan et al., 2003), GABA (Wang et al., 2002), 2 arachidonoylglycerol (Walter et al., 2004), and purines (Ballerini et al., 1996) from cultured astrocytes. In neurological diseases or injuries extracellular ATP may activate P2X7Rs further enhancing purine release, with important pathophysiological consequences (Ballerini et al., 1996). In paraventricular nucleus of hypothalamus, P2X7 receptor activation results in insertion of AMPA receptors into postsynaptic neuronal membrane (Gordon et al., 2009).

(Vavra et al., 2011).

hippocampal slices (Papp et al., 2004).

In the rat suprachiasmatic nuclei, ATP content and extracellular ATP levels (Yamazaki et al., 1994; Womac et al., 2009) negatively correlate with electrical activity and arginine vasopressin (AVP) secretion rhythm: the frequency of action potentials and the level of AVP is high during the day and low during the night, but ATP release peaks during the night. This might indicate that ATP is primarily stored and released from distinct pool of vesicles and/or that ATP and AVP are released from different cells. How ATP release from hypothalamic cells is regulated and what is the reason for local differences in distribution of P2X receptor subtypes is still being elucidated.
