**2.9 Quantitative characters in M2 and M3 generations**

Gamma radiations and GR + EMS proved to be very effective to induce variability in quantitative traits like plant height, primary branches per plant, days required for first flowering and first pod maturity, number of pods per plant, pod length, number of seeds per pod, 1000 seed weight and yield per plant in M2 and M3 generations (Table-3 and 4 ).

#### **2.10 Quantitative traits**

#### **2.10.1 Plant height**

The treatments of gamma radiations and combinations were effective for reducing the plant height (Table-3) and the maximum reduction was noted in 100Gy.

Induction of Genetic Variability

**Plant height (cm)** 

**Pri. br./ plant** 

**DAS for first flowering**

Table 3. Micromutations in M2 generation of horsegram cv. Dapoli Kulthi-1.

Data obtained in M2 generation on number of primary branches per plant (Table-3) indicated that the mean values of this parameter showed positive and negative influence. Maximum number of primary branches per plant was recorded in 300Gy (7.66) over

All the treatments in M3 showed increase in number of primary branches per plant. Maximum number was recorded in 100Gy (12.58) and 200Gy + 0.2 %EMS (11.25) over control (10.99). Dalvi (1990) also noted similar trend with physical as well as chemical

GR and GR + EMS treatments have induced the variability in number of days required for first flowering in M2 generation. However some treatments were stimulatory and others were inhibitory for inducing the flowering. The minimum number of days required for first

**2.11 Number of primary branches per plant** 

**2.12 Number of days required for first flowering** 

flowering were 50 DAS in 300Gy and 42 DAS in 400Gy + 0.4%EMS.

**Treat** 

100Gy +

control.

mutagens in horsegram.

with Gamma Radiation and Its Applications in Improvement of Horsegram 217

**DAS for first pod maturity**

Control 46.70±1.87 6.30±0.25 53.30±2.13 85.20±3.41 71.20±2.85 7.20±0.29 26.80±1.07 14.54±0.58 100Gy 35.40±1.77 6.66±0.33 53.07±2.65 81.70±4.09 67.70±3.39 6.66±0.33 28.30±1.42 13.36±0.67 200 39.40±2.76 6.20±0.43 53.70±3.76 78.90±5.52 59.50±4.17 6.33±0.44 28.20±1.97 11.22±0.79 300 41.30±1.24 7.66±0.23 54.80±1.64 84.60±2.54 83.80±2.51 7.10±0.21 27.50±0.83 16.76±0.50 400 40.10±2.41 6.10±0.37 54.70±3.28 84.40±5.06 71.30±4.28 6.33±0.38 26.30±1.58 12.87±0.77

0.2%EMS 28.70±1.72 6.10±0.37 49.40±2.96 79.20±4.75 38.40±2.30 6.20±0.37 25.60±1.54 8.83±0.53 100 + 0.3 29.40±1.18 8.40±0.34 47.60±1.90 77.30±3.09 49.70±1.99 6.10±0.24 26.50±1.06 7.64±0.31 100 + 0.4 26.50±1.86 5.60±0.39 47.30±3.31 79.70±5.58 37.80±2.65 6.30±0.44 26.20±1.83 8.46±0.59 100 + 0.5 31.20±0.94 5.10±0.15 47.50±1.43 78.60±2.36 41.40±1.24 6.00±0.18 27.50±0.83 8.29±0.25 200 + 0.2 33.20±1.66 5.90±0.30 49.30±2.47 77.80±3.89 38.70±1.94 6.50±0.33 27.60±1.38 8.87±0.44 200 + 0.3 25.70±0.77 6.10±0.18 49.40±1.48 79.30±2.38 41.30±1.24 6.20±0.19 26.60±0.80 9.12±0.27 200 + 0.4 27.50±1.65 6.20±0.37 47.60±2.86 79.80±4.79 40.70±2.44 6.00±0.36 27.80±1.67 9.76±0.59 200 + 0.5 30.20±2.11 7.10±0.50 49.20±3.44 78.50±5.50 39.80±2.79 6.50±0.46 26.30±1.84 8.90±0.62 300 + 0.2 34.30±1.37 6.50±0.26 54.90±2.20 79.40±3.18 54.80±2.19 7.10±0.28 26.40±1.06 13.10±0.52 300 + 0.3 31.20±1.56 7.10±0.35 54.40±2.72 79.60±3.98 50.70±2.54 7.00±0.35 26.60±1.33 11.53±0.58 300 + 0.4 29.10±2.04 5.20±0.36 54.30±3.80 78.70±5.51 33.50±2.35 7.10±0.50 30.50±2.14 9.98±0.70 300 + 0.5 35.30±1.06 9.10±0.27 50.80±1.52 80.60±2.42 71.70±2.15 7.10±0.21 29.80±0.89 16.01±0.48 400 + 0.2 42.50±2.13 7.800.39± 50.40±2.52 76.80±3.84 71.40±3.57 6.50±0.33 28.70±1.44 14.44±0.72 400 + 0.3 33.20±1.99 6.60±0.40 56.30±3.38 77.60±4.66 70.40±4.22 7.10±0.43 25.80±1.55 12.69±0.76 400 + 0.4 40.90±1.64 6.70±0.27 40.80±1.63 57.70±2.71 52.80±2.11 6.20±0.25 27.70±1.11 10.38±0.42 400 + 0.5 47.50±1.43 8.30±0.25 40.60±1.22 66.80±2.00 74.40±2.23 6.20±0.19 27.70±0.83 14.10±0.42 SEM± 1.42 0.27 2.14 3.30 2.25 0.27 1.41 0.47 F-value 42.03 29.99 8.26 3.64 95.80 4.82 2.45 69.09 P-value 0.00 0.00 0.00 0.00 0.00 0.00 0.01 0.00 LSD 0.05 2.78 0.53 4.19 6.47 4.41 0.53 2.76 0.92

**No. of pods/ plant** 

**No. of seeds/ pod** 

**1000 seed Wt. (g)** 

**Seed yield/ plant (g)** 

Plate 3. Pod mutants.

Plate 3. Pod mutants.


Table 3. Micromutations in M2 generation of horsegram cv. Dapoli Kulthi-1.
