**1.1 Experimental layout**

The authentic seeds of horsegram (*Macrotyloma uniflorum* (Lam.) Verdc) cultivar Dapoli Kulthi-1 were procured from Head, Department of Botany, College of Agriculture, Dr. Balasaheb Savant Konkan Agricultural University, Dapoli, Dist-Ratnagiri (M.S.) India.

The field experiments were conducted on the experimental field at Department of Botany, University of Pune, Pune 411 007 (M.S.). The crop of horsegram Dapoli Kulti-1 was grown in *Kharif* season under uniform conditions. All the experiments were carried out in triplicate, following RBD design. The size of each plot was 3.75m X 2.75m and each plot had 225 plants. The distance between two rows and two plants was 30 X 15 cm and the distance between two adjacent plots was one meter.

A total of 42 combination treatments in M1 generation with untreated dry seeds, which was used as control. Final 675 seeds of each treatment were used to raise M1 generation. The M2, M3, M4 and mutants were raised in next rainy season.
