**2.4 pH**

312 Gamma Radiation

incidence of obesity and cardiovascular disease. Today consumers already have access to food at the same time convenient and healthy. The light processing adds to the fruit (products healthy by nature) the value of convenience. With the advancement of technology it is possible to find in the market peeled and sliced fruit or juice form with cool features ready for consumption (Demczuk Junior, 2007; Oliveira, 2011; Oliveira et. al.,

The principal processed products obtained from fruits are juices and nectars (sweetened drink). In this work, a no-alcoholic sweetened drink was prepared from Kiwi, containing 50% pure juice and sugar, and ready to consume (Tocchini, 1995), which was treated with

The aim of this work was to formulate a no-alcoholic sweetened drink, starting from kiwi fruits and to submit the drink to gamma radiation derived from a source of Cobalt-60, and

Kiwi fruits (Actinidia deliciosa) in natura were locally purchased. The fruits were washed in a bleach solution, peeled, and cut in half. The juice was extracted by home centrifuge Walita, pre-filtered, centrifuged at 5000 rpm, and filtered. Mineral water was added to 50% volume, and sucrose to reach 16º Brix. It was made an ultra filtration with ceramic membrane of 0.10µm at the pressure of 3bar at 45ºC for clarification, according to Lopes et al. (2005). The drink was kept in 500 mL plastic bottles (PET), and exposed to the

Irradiation was conducted in a Cobalt-60 source; model Gammabeam 650, of Nordion Canada, installed in the Center of Nuclear Energy in Agriculture of the University of Sao Paulo – CENA/USP., Piracicaba city, State of São Paulo, Brazil. The dose rate was 0.712 kGy/hour. The analyses were conduced in the Laboratory of Radiobiology and

Dosimetry was performed using 5 mm diameter alanine dosimeters (Bruker Instruments, Rheinstetten, Germany), and the free radical signal was measured with Bruker EMS 104 EPR Analyzer. The actual dose was within 0.02 of the target dose. Samples was turned 360 continuously during the irradiation process to achieve uniform target doses and the nonirradiated control was placed outside the irradiation chamber to have the same

Estimated in refractometer RT-30ATC and expressed n Brix, according to AOAC (1995).

environmental temperature effect with the irradiating sample.

evaluates changes in physical and chemical quality attributes cause by irradiation.

2011).

irradiation at 0.5; 1.0 and 2.0 kGy doses.

**2. Material and methods** 

following treatments:

2. Irradiation with 0.5 kGy; 3. Irradiation with1.0 kGy 4. Irradiation with 2.0 kGy

Environment of CENA/USP.

**2.1 Physico-chemical analyses** 

**2.2 Soluble solids content** 

1. Control;

Determined using pHmeter MB-10, according to AOAC (1995).
