Significantly reduced (P0.05) compared to control.

ALT, Alanin Amino transferase

AST. Aspartate amino transferase

ALK. Phos, Alkaline Phosphatase.

Table 5. Effects of *V.Amygdalina* on Clinical Chemistry Parameter in rats. (14days exposure)

Herbal Medicine in the Treatment of Malaria:

**3.7 Hematological analysis** 

leave of *V.amygdalina.* 

*Vernonia amygdalina*: An Overview of Evidence and Pharmacology 181

Table 6. Mean hematology values of rats after repeated (6 weeks) oral dosing with extract

mg/kg/d) and (8.02 ± 0.7 in 1500 mg/kg/d) versus control (4.80 ± 0.8).

Doses mg/kg WBC (109/ l) RBC (1012/l) HGB (g/dl) HCT (%) MCV (fL) MCH (pg) MCHC (g/dl) PLT (109

Control 4.80.8 8.41.2 16.92 446 534 202 382 42950 6.70.1 0.50.01 4.10.7

750mg/kg 6.61.0 8.71.1 17.23 451 524 191 384 46418 6.51.0 1.00.4\* 5.40.8

1500mg/kg 8.00.7\* 8.20.6 16.71 433 532 200.3 383 55543 6.50.1 0.70.1 6.90.6\*

3000mg/kg 8.50.3\* 8.40.2 17.10.2 448 523 200.3 383 436161 6.70.3 0.70.1 7.30.3\*

WBC = White blood cell; RBC = Red blood cell; HGB = Hemoglobin; HCT = Hematocrit; MCV = Mean corpuscular volume; MCH = Mean

corpuscular hemoglobin; MCHC = Mean corpuscular hemoglobin concentration; PLT = Platelets; NE = Neutrophil; LY = Lymphocyte

//)

(fL)

(%)

(%)

MPV

NE

LY

mg/kg/d) and (6.94 0.6 in 1500 mg/kg/d) as compared to control (4.13 0.7)

The WBC count presented a significant increase in the two high doses (8.47 ± 0.3 in 3000

Values presented as mean standard deviation.

Statistical analysis: ANOVA, then Dunnett's test

\*Mean value of group is significantly different from control at p<0.05

n = 7

There was a significant increase in the lymphocyte values in high doses (7.33 0.2 in 3000

The mean values of the nine haematological parameters are reported in (Table 6)

Weeks

Fig. 5. Evolution of the mean ±SE of rat body weight during the subchronic toxicity of *Vernonia amygdalina* extract.

Fig. 6. Plots of the mean SE. Of the harvested organ weights during the sub-chronic toxicity study of *V.amygdalina* extract.
