**Experimental and Computational Methods Pertaining to Surface Tension of Pharmaceuticals**

Abolghasem Jouyban1 and Anahita Fathi-Azarbayjani2 *1Drug Applied Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, 2Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran* 

#### **1. Introduction**

46 Toxicity and Drug Testing

Wurst, F., Dresen, S., Allen, J., Wiesbeck, G., Graf, M. & Weinmann, W. (2006). Ethyl

*Addiction,* Vol.101, No.2, (February 2006), pp.204-211, ISSN 1360-0443 Wurst, F., Wiesbeck, G., Metzger, J., Weinmann, W. & Graf, M. (2004). On Sensitivity,

*Research,* Vol.28, No.8, (August 2004), pp.1220-1228, ISSN 1530-0277

Sulphate: A Direct Ethanol Metabolite Reflecting Recent Alcohol Consumption.

Specificity, and the Influence of Various Parameters on Ethyl Glucuronide Levels in Urine - Results from the Who/Isbra Study. *Alcoholism, Clinical and Experimental* 

> The molecules of a fluid experiences attractive forces exerted on it by all its neighboring molecules. In the bulk of the liquid, molecules are attracted equally in all directions resulting in a net force of zero. Molecules at or near the surface experience attractive force which tends to pull them to the interior. Surface chemistry deals with thermodynamic and kinetic parameters that take place between two different coexisting phases at equilibrium. Surface tension, γ is free energy of the surface at any air/fluid interface defined as force per unit length or energy per unit area. The latter term, also called surface energy, is more useful in thermodynamics and it applies to solids as well as liquid surfaces. The surface free energy of a liquid is measured by its surface tension and the surface free energy of a solid can be revealed by contact angle measurements. The surface tension measurement depends very markedly upon the presence of impurities in the liquid, temperature and pressure changes (Buckton, 1988).

> Surface tension is a phenomenon that we see in our everyday life. Human biological fluids, e.g. serum, urine, gastric juice, amniotic liquid, cerebrospinal and alveolar lining liquid contain numerous low-and high-molecular weight surfactants, proteins and lipids that adsorb at liquid interface. The physicochemical processes that take place in these interfaces are extremely important for the vital function of body organs and have a great impact on pharmacodynamic parameters of drug molecules (Kazakov et al., 2000; Trukhin et al., 2001). Drug substances are usually administered as part of a formulation in combination with excipients that have varied and specialized pharmaceutical functions. The design of drug formulation is based on the principles of pharmacokinetic, biopharmaceutic and pharmaceutical technology. The pharmaceutical industry has directed its attention mainly to the quality and processability of active pharmaceutical products which is reflected in various physicochemical parameters. The drug and pharmaceutical materials require extensive characterization and testing of their stability, physico-chemical properties, effectiveness, palatability and ease of administration to ensure drug efficacy. Among the important characteristics are the drug dissolution and solubility in gastrointestinal tract, intestinal absorption, drug distribution and drug-

Experimental and Computational Methods Pertaining to Surface Tension of Pharmaceuticals 49

meniscus formed at the solid–liquid interface is characterized by the contact angle. Two contact angles are measured, an advancing contact angle and a receding contact angle and the surface tension is calculated from the resulting force. The wetting force is monitored with time and this method is specially suited to check static surface tension value which in some cases is more than 4 h, hence the measurements are assumed to represent equilibrium. The main drawback of this method is that the surface age (time taken from surface formation till measurement) is not taken into account. When working with viscous liquids it takes time for a viscous material to flow from the dipped portion of the plate. Thus the surface tension will decrease initially till it reaches a pleatue once the excess liquid has flowed from the plate, therefore this method is not suited for highly viscous solutions (Avranas and Taspoulos, 2000; Krishnan et al., 2005; Santos and Castanho, 2004). The static wetting force on the plate is used to calculate the static surface tension (γ) using the

> .cos *F l*

where *F* is the difference in wetting force upon immersion and withdrawal in mN/m, *l* is

platinum plate or clean glass is often assumed to be zero. This method does not require other correction factors such as fluid density. This method can be used to measure the contact angle and wetting properties of solid surfaces, where the platinum ring plate is replaced by the test surface (Avranas and Tasopoulos, 2000; Krishnan et al., 2005; Santos and

pharmaceutics. Contact angle measurements span every pharmaceutical field, from fluid dynamic to powder and tablet (Muster and Prestidge, 2002), adhesion and spray-drying of various drug delivery systems (Millqvist-Fureby et al., 1999) to the detection of impurities in the solutions of surface active compounds (Al-Maaieh and Aburub, 2007). It is of particular interest in powders, because the formulations are dependent on the contact angle. Contact angle measurements can be performed using various methods including Wilhelmy plate method, capillary rise method, goniometer and sessile drop method (Dingles and Harris, 2005). Among the interfacial tensiometry methods, the Wilhelmy plate method has been extensively developed and used by scientists in the pharmaceutical industry. The equipment used in this method is commercially available at several companies. With this technique it is possible to measure and control interfacial properties in granulation and tabletting, (Dreu et al., 2005) polymeric surfactants, emulsions and foams, protein-phospholipid interaction (Oritz et al., 2003), interfacial tension of topical skin formulations (Vejnovic et al., 2010), bioadhesive forces between mucosal tissue and microsphere drug delivery system (Vasir et

Du Noüy ring method is a traditional method used to measure static surface or interfacial tension. The measurement simply requires the ring to be wetted by the liquid and then pulled through the interface while measuring the force exerted on the ring (see Figure 1). Wetting properties of the surface or interface have little influence on this measuring technique. As in the case of Wilhelmy plate, the ring, with a diameter of 2-3 cm, is usually

(1)

, is an important parameter in many industries including

is the advancing or receding contact

of most liquids against

Wilhelmy equation:

the wetted perimeter of the Wilhelmy plate and

Castanho, 2004; Sipahi, 2001; Tan et al., 2005).

The static contact angle,

**2.2 Du Noüy ring method** 

al., 2003).

angle between the liquid phase and the plate. The contact angle

plasma protein binding. By applying special surface treatments such as contact angle and surface tension measurements to pharmaceutical compounds, drug distribution, dissolution behavior and release pattern in various body fluids can be improved (Hancock et al., 1997; Ho et al., 2010).

Surface tension can influence the development, prediction and performance of pharmaceutical products and help to solve industrial problems and improve products quality. Due to the importance of this phenomenon in drug formulations, there is a growing need for specific interfacial consideration that can be used routinely to solve pharmaceutical problems and improve product quality and stability. In order to meet challenges and develop new and better performing pharmaceutical products, knowledge of surface tension and its measurements techniques is of utmost importance. Amongst many techniques used for characterizing the surface energies of pharmaceuticals are the surface tension measurements, contact angle and wettability tests (Buckton, 1988; Chamarthy et al., 2009; Puri et al., 2010). The objective of this chapter is to introduce experimental and computational methods of surface tension measurment in the pharmaceutical industry.
