**3.8 Examples of equipment used**

The BioCool Controlled Rate Freezer from FTS Systems/SP Scientific is a mechanically refrigerated bath with temperature control to -40 °C or -80 °C. The fluid in the 2 litre bath provides temperature stability and dispersion of the heat of fusion without a concomitant temperature rise. Courtesy SP Scientific and Gary Gold Photography.

BioCool™ Controlled Rate Freezer

The BioCool Controlled Rate Freezer from FTS Systems/SP Scientific is a mechanically refrigerated bath with temperature control to -40 °C or -80 °C. The fluid in the 2 litre bath provides temperature stability and dispersion of the heat of fusion without a concomitant

temperature rise. Courtesy SP Scientific and Gary Gold Photography.

Fig. 3. Single solution mode.

**3.8 Examples of equipment used** 

BioCool™ Controlled Rate Freezer

The Asymptote EF600, a unique liquid nitrogen free, controlled-rate freezer, is electrically powered by a Stirling Cycle Cryocooler rather than liquid nitrogen. This allows the freezer to be used where liquid nitrogen is in short supply, where extra high air quality is needed, or where there is a risk of LN2 contamination to samples.

Asymptote liquid nitrogen free controlled-rate freezer

The Planer Kryo 360 cell freezer controls down to a -180 °C end temperature to ensure sample integrity during transfer to storage. Fully programmable, it allows the use of protocols associated with the most advanced cryopreservation techniques and is widely used in laboratories around the world.

Planer Kryo 360 programmable cell freezer

The Crysalys controlled rate freezer: programmes, time and temperatures may be entered via a touch screen with up to 100,000 cycles held on the onboard SD card; the data can be retrieved by any PC or Mac computer. A battery back up operates the system for 3 hours; its portability and 3.2 kg weight make it especially suited to veterinary purposes.

Precision in Cryopreservation – Equipment and Control 525

The Planer ShipsLog is a datalogger specifically designed for vapour shippers, which

The Liquidus Tracker is a new controlled vitrifier for cryopreservation of samples using the liquidus tracking technique. This approach may have uses in vitrifying larger samples and

We would like to thank Dr. Damian Marshall, Principal Scientist, LGC Ltd, Teddington, UK and Mr Ian Pope, Coreus Consulting, Faribault, MN, USA, for their valuable assistance with

Additional material can be found in 'Cryopreservation and Freeze-Drying Protocols'; Day, J.; Stacey, G. (Eds.); 2nd ed., 2007 Humana Press Cryopreservation of Animal and Human Cell

Chapter 16; Morris, C; Cryopreservation of Hematopoietic Stem/Progenitor Cells;

maintains a downloadable temperature history of samples during transit.

ShipsLog datalogger for vapour shippers

those which are currently difficult to cryopreserve.

Liquidus Tracker controlled vitrifier for cryopreservation

Chapter 3; Pegg,D.E. Principles of Cryopreservation.

Chapter 17; Watt S., Austin E., Armitage S.,

**4. Acknowledgement** 

this chapter.

Lines; See

Crysalys controlled rate freezer

The Gemini Tinytag View 2 data logger, when used with a specially designed probe, is used for temperature monitoring in cryogenic environments down to -200 °C.

Tinytag View 2 data logger

The CoolCell is an alcohol-free cell freezing container which provides a reproducible cooling rate of 1 °C/minute when placed in a -80 °C freezer. No alcohol is required to control the freeze rate as the design and materials of the CoolCell ensure precise and uniform heat removal from cryovials.

CoolCell®, an alcohol-free cell freezing container

The Gemini Tinytag View 2 data logger, when used with a specially designed probe, is used

The CoolCell is an alcohol-free cell freezing container which provides a reproducible cooling rate of 1 °C/minute when placed in a -80 °C freezer. No alcohol is required to control the freeze rate as the design and materials of the CoolCell ensure precise and uniform heat

for temperature monitoring in cryogenic environments down to -200 °C.

Crysalys controlled rate freezer

Tinytag View 2 data logger

removal from cryovials.

CoolCell®, an alcohol-free cell freezing container

The Planer ShipsLog is a datalogger specifically designed for vapour shippers, which maintains a downloadable temperature history of samples during transit.

ShipsLog datalogger for vapour shippers

The Liquidus Tracker is a new controlled vitrifier for cryopreservation of samples using the liquidus tracking technique. This approach may have uses in vitrifying larger samples and those which are currently difficult to cryopreserve.

Liquidus Tracker controlled vitrifier for cryopreservation
