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**15** 

*Japan* 

Daisuke Kami

**Cryopreservation of** 

**Plant Genetic Resources** 

*National Agricultural Research Center for Hokkaido Region* 

The plant genetic resources is preserved by pollen, seed, branch, bulb, or tissue culture at the gene bank. Since these need to be updated periodically (several months ~ several years), I point out the problems involving the great labor and space spent for the maintenance of

Cryopreservation is a storage method of plant genetic resources at ultra-low temperature, for example, that of liquid nitrogen (LN; -196 oC). It is a preservation method that enables

For successful cryopreservation, it is essential to avoid intracellular freezing and induce the vitrification state of plant cells during cooling in LN. In addition, the cryopreservation method should be a simple protocol for everyone to use easily. Since the 1970's, cryopreservation techniques have been researched using different plant organs, tissues and cells. As a result, different cryopreservation procedures have been developed (for example, slow-prefreezing method, vitrification method, dehydration method). With the development of these cryopreservation methods, tissues of tropical plants, which have been conventionally thought to be not cryopreserved, also were successfully preserved in LN (Bajaj, 1995; Towill & Bajaj, 2002). In this Chapter, I describe different types of

In addition, I often ask my colleagues why cryopreservation of plant tissue did not succeed irrespective of the method. As the cause, it is possible that there is a problem in the character of plant species (stress resistance and polyphenol production), or the ways used in the cryopreservation technique. Then, I also would like to present some knowledge about some improvements for making cryopreservation of plant genetic resources more successful in

In the section (2.1), I would like to introduce cryopreservation methods of plant genetic resources that have been developed. In the section (2.2), I would like to describe the approach when cryopreserving plant samples from the past literatures or my own

plant genetic resources to be conserved safely, and cost-effectively.

**2. Methods of cryopreservation of plant genetic resources** 

experience in order to enhance the regrowth percentage after cryopreservation,

**1. Introduction** 

plant genetic resources.

cryopreservation methods.

this chapter.

