**3. Free radical and oxidative damage in plant cryopreservation**

While achieving an optimum cryopreservation protocol that successfully avoids ice damage is important, there are various other factors that can affect post-cryogenic survival (Fig. 2). During the cryopreservation procedure plant tissues are susceptible to a variety of stresses, including oxidative stresses (Benson & Bremner, 2004).

Fig. 2. Main causes of damage to plant tissues during cooling and cryopreservation (modified from [Turner, 2001]).
