Contents

## **Preface XIII**

	- **Part 2 Stem Cells and Cryopreservation in Regenerative Medicine 137**
	- **Part 3 Human Assisted Reproduction Techniques (ART) 167**

X Contents


Contents VII

Chapter 17 **Cryopreserving Vegetatively Propagated Tropical Crops –** 

Chapter 18 **Precision in Cryopreservation – Equipment and Control 507**

Edoardo Lopez, Katiuscia Cipri and Vincenzo Naso

Agnieszka Partyka, Wojciech Niżański and Małgorzata Ochota

Chapter 20 **Methods of Assessment of Cryopreserved Semen 547** 

**and** *Solenostemon rotundifolius* **485** 

Elizabeth Acheampong and Marceline Egnin

**The Case of** *Dioscorea* **Species**

Marian D. Quain, Patricia Berjak,

Stephen Butler and David Pegg

**Overview and Innovation 527**

Chapter 19 **Technologies for Cryopreservation:** 

**Part 8 Equipment and Assays 505** 

	- **Part 4 Farm / Pet / Laboratory Animal ART 229**
	- **Part 5 Cryopreservation of Wildlife Genome (Terrestrial Animals) 291**
	- **Part 6 Cryopreservation of Aquatic Species 389**
	- **Part 7 Cryopreservation of Plants 415**

#### Chapter 17 **Cryopreserving Vegetatively Propagated Tropical Crops – The Case of** *Dioscorea* **Species and** *Solenostemon rotundifolius* **485**  Marian D. Quain, Patricia Berjak, Elizabeth Acheampong and Marceline Egnin

#### **Part 8 Equipment and Assays 505**

VI Contents

Chapter 7 **Oocyte Cryopreservation for the Elective** 

Chapter 8 **Cryopreservation of Testicular Tissue 209**

**Part 4 Farm / Pet / Laboratory Animal ART 229**

Heriberto Rodriguez-Martinez

Chapter 10 **Cryopreservation of Embryos from** 

Chapter 11 **Genome Banking for Vertebrates** 

Joseph Saragusty

Chapter 9 **Cryopreservation of Porcine Gametes, Embryos and Genital Tissues: State of the Art 231**

> **Model Animals and Human 259**  Wai Hung Tsang and King L. Chow

**Part 5 Cryopreservation of Wildlife Genome (Terrestrial Animals) 291**

**Wildlife Conservation 293** 

Regina Celia Rodrigues da Paz

Chapter 12 **Wildlife Cats Reproductive Biotechnology 369** 

**Part 6 Cryopreservation of Aquatic Species 389**

Loredana Zilli and Sebastiano Vilella

Chapter 14 **Current Issues in Plant Cryopreservation 417**

Chapter 15 **Cryopreservation of Plant Genetic Resources 439** 

Chapter 16 **Cryopreservation of Spices Genetic Resources 457**

P.N. Ravindran and K.V. Peter

K. Nirmal Babu, G. Yamuna, K. Praveen, D. Minoo,

**Part 7 Cryopreservation of Plants 415**

Daisuke Kami

Chapter 13 **Effect of Cryopreservation on Bio-Chemical Parameters,**

**of Proteins of Seawater Fish Spermatozoa 391**

Anja Kaczmarczyk, Bryn Funnekotter, Akshay Menon,

**DNA Integrity, Protein Profile and Phosphorylation State** 

Pui Ye Phang, Arwa Al-Hanbali, Eric Bunn and Ricardo L. Mancera

Ali Honaramooz

**Preservation of Reproductive Potential 185** Catherine Bigelow and Alan B. Copperman


#### Chapter 20 **Methods of Assessment of Cryopreserved Semen 547**  Agnieszka Partyka, Wojciech Niżański and Małgorzata Ochota

Preface

well.

started their journey to this fascinating science.

Almost a decade has passed since the last textbook on the science of cryobiology and the most common methods of cryopreservation was published [Fuller *et al*, 2007], to which we will refer as *"the previous book"* here and below. When it was published, it became a useful guide for both "seasoned" cryobiologists and those who had just

However, there have been some serious tectonic shifts in cryobiology, which were perhaps not seen on the surface but may have a profound effect on both the future of cryobiology and on the development of new cryopreservation methods. We feel that it is time to revise the previous paradigms and dogmas, discuss the conceptually new cryobiological ideas and introduce the recently emerged practical methods of cryopreservation. The present books, *Current Frontiers in Cryobiology* [Katkov, 2012A] (referred here as *Book 1*) and *Current Frontiers in Cryopreservation* [Katkov, 2012B] (*Book 2*), will serve the purpose. These two books are not a substitute for *the previous book* but are rather complementary, so we highly recommend to all readers who want to know the background on which *the current books* were written to read *the previous book* as

Before we describe the current books, let us first briefly compare them to the previous book in retrospective. First of all, there were some very promising directions a decade ago that unfortunately did not meet the expectations. Molecular biology and genetics, particularly in regards to expression of stress proteins and other pathways related to the cell injury, have not introduced any serious breakthroughs except for the use of ROCK inhibitors for cryopreservation of human embryonic and induced pluripotent stem cells. The latter really was a revolutionary discovery, which however, was not made by cryobiologists; it was just *"*picked up*"* by them from the Watanabe's seminal work [Watanabe *et al*, 2007] (see the Chapter by Martin- Ibáñez in *Book 1* for details). In general, however, all those molecular biology tools have helped the solution but have not solved the cryopreservation problems *per se*. One of the backlashes of this new era is that the "traditional" cryobiologists now have little chances of getting a grant from many funding agencies such as NIH, whose panels are dominated by molecular biologists and geneticists, unless the applicant is willing to study those pathways and use of transcriptomics, proteomics, metabolomics, and other *"*omics*"*. Yet, all those very expensive tools have so far added a little to the science of cryobiology, and
