**Meet the editor**

Igor I. Katkov, PhD, is a "seasoned" cryobiologist with more than 30 years of experience in the field. He received his MSc and PhD diplomas in Kharkov, Ukraine. He has collaborated with many prominent cryobiologists, including P. Mazur, F. Ostashko, V. Grischenko, the Isachenkos, and many others. Dr Katkov has more than 130 publications and is actively working in the

fields of hyper-fast kinetic vitrification, osmotic modeling, cryopreservation of adherent cells, and many other fields related to fundamental cryobiology, assisted reproduction and regenerative medicine. Igor Ivanovich is a renowned scientist and a member of Editorial Boards of the "Cryo Letters" and "Problems of Cryobiology and Cryomedicine" journals.

Contents

**Preface IX** 

**Part 1 Basic Cryobiology and Kinetic Vitrification 1** 

Chapter 1 **Kinetic Vitrification of Spermatozoa of Vertebrates: What Can We Learn from Nature? 3** 

S.I. Moskovtsev, M.P. Petrushko, V.I. Pinyaev,

Chapter 2 **Vitrification Technique – New Possibilities** 

M. Schulz, V. Isachenko and R. Sànchez

S.I. Moskovtsev, A.G-M. Lulat and C.L. Librach

**During Addition and Removal of Cryoprotective** 

Raquel Martín-Ibáñez, Outi Hovatta and Josep M. Canals

**Part 3 Human Assisted Reproduction Techniques (ART) 167** 

Chapter 3 **Cryopreservation of Human Spermatozoa by** 

Chapter 4 **Prevention of Lethal Osmotic Injury to Cells** 

Dayong Gao and Xiaoming Zhou

Chapter 6 **Vitrification of Oocytes and Embryos 169** 

Juergen Liebermann

**Part 2 Stem Cells and Cryopreservation in Regenerative Medicine 137** 

**Agents: Theory and Technology 101** 

Chapter 5 **Cryopreservation of Human Pluripotent Stem Cells: Are We Going in the Right Direction? 139** 

I.I. Katkov, V.F. Bolyukh, O.A. Chernetsov, P.I. Dudin, A.Y. Grigoriev, V. Isachenko, E. Isachenko, A.G.-M. Lulat,

K.M. Sokol, Y.I. Sokol, A.B. Sushko and I. Yakhnenko

**for Male Gamete Low-Temperature Storage 41**  E. Isachenko, P. Mallmann, G. Rahimi, J. Risopatròn,

**Vitrification** *vs.* **Slow Freezing: Canadian Experience 77** 
