**7. References**


γ-terpinene[Sonboli et al., 2005] were responsible of antioxidative and antiseptic activities of essential oils studied. However, caryophyllene oxide, E-nerolidol humulene epoxide-1,2 possessed antiinflammatory activity [Chavan et al., 2010; Yu-Tang et al., 2008; Wanjohi Mwangi et al., 2009] Possible synergistic and antagonistic effects compounds in *V. simplicifolia* essential oil should also be taken into consideration. These reports are

This study shows in *vivo* wound healing activity and in *vitro* bacteriostatic effect of *Vitex simplicifolia* essential oil. The oil demonstrates the strongest wound cicatrization activity than cicatryl and natural immunity. In addition the oil may help to prevent wound infections and others such diarrhoea, dysentery and skin diseases. These results indicate that the plant could be use as a natural potential remedy for healing wounds and antiseptic agent. Further investigations will be performed by determination of analgesic, antioxidant and anti inflammatory activities of the essential oil and to expand to other

1. Choice of rabbits: we have chosen rabbits because of they were available in the laboratory and cheaper. They were also very easy to be used in the cicatrization effect

2. The resolution of photographs depend of the quality of the apparatus, we deleted them because we have not a best quality. We are sorry for the bad quality of photos. Thank

Bassole, I.H.N., Ouattara, A.S., Nebié, R., Ouattara, C.A.T., Kaboré, Z.I., Traoré, S.A., 2003.

Bruneton, J., 1993. Pharmacognosy, phytochemistry, medicinal plants. 2nd Tech and Doc.

Chattopadhyay, D., Arunachalam, G., Mandal, A.B., Sur, T.K., Mandal ,S.C., Bahattacharya,

Chavan, M.J., Wakte, P.S., Shinde, D.B., 2010. Analgesic and anti-inflammatory activity of

Draize, J.H., Woodward, G., Calvery, H.O., 1944. Methods for study of irritation and toxicity

peltatus leaf extract. Journal of Ethnopharmacognosy 82, 229-237

journal of phytotherapy and phytopharmacology 17(2), 149-151

Pharmacology and Experimental Therapeutics 2, 377-390

and *Lippia multoflora*. Phytochemistry 62, 209-212

Chemical composition and antibacterial activities of essential oils of *Lippia chevalieri*

S.K., 2002. Antimicrobial and anti inflammatory activity of folklore: Mellotus

Caryophyllene oxide from Annona squamosa L. bark. Phytomedicine international

of substances applied topically to the skin and mucous membranes*.* Journal of

compatible with our results in the present study.

**5. Conclusion** 

*Vitex* species.

**6. Remarks** 

**7. References** 

than mice and rats

you for your understanding.

Lavoisier Paris p915


**7** 

 *Brasil* 

**Use of Associated Chromatographic** 

 **of Bioactive Monoterpenoid Indole** 

*2State University of Campinas, Chemistry Institute, Campinas-SP,* 

**Techniques in Bio-Monitored Isolation** 

**Alkaloids from** *Aspidosperma ramiflorum*

Talita Perez Cantuaria Chierrito1, Ananda de Castro Cunha1, Luzia Koike2, Regina Aparecida Correia Gonçalves1 and Arildo José Braz de Oliveira1 *1Department of Pharmacy, State University of Maringá, Maringá, Paraná State,* 

The genus *Aspidosperma* (Apocynaceae) have been commonly used in folk medicine as potential antimalarial agents; in the treatment of leishmaniasis; uterus and ovary inflammations; as a contraceptive; in diabetes; stomach disorders; against cancer; fever and rheumatism (Oliveira et al, 2009). It commonly grows in tropical America, extracted from trees ranging 2 to 60 m in height. It is found in a variety of habitats from the dry fields of south-central Brazil, Paraguay, and Argentina to the inundated river margins of the Amazon basin (Tanaka, 2006). The main constituents of the *Aspidosperma* genus are indole alkaloids, a class of substances with a wide range of pharmacological activities such as cholinesterase inhibitors, analgesic, anti-inflammatory, bactericidal, oestrogenic, stimulant

Several methods are available for the analysis and identification of known indole alkaloids. Analysis of complex mixtures is frequently done by means of Thyn Layer Chromatography (TLC), through comparison of Rf values obtained in different solvent systems, and also by comparison between specific color reactions of components of the mixture and reference compounds. TLC remains one of the preferred methods for qualitative analysis of known compounds since it requires neither sophisticated equipment nor extensive sample preparation. For quantitative analysis, High Performance Liquid Chromatography (HPLC) systems linked to a UV detector are commonly used. By coupling HPLC to a photodiode array UV detector makes it possible to combine the information over retention times and the UV spectrum of each compound, and in some cases, it also enables the quantification of overlapping peaks. Capillary Gas Chromatography (GC) analysis has been described for several classes of alkaloids. A major advantage of GC over the above-mentioned methods is its enhanced sensitivity and high resolution. Another advantage is its easy coupling to a

and depressant of the central nervous system (CNS) (Zocoler et al, 2005).

**1.1 Chromatographic systems in** *Aspidosperma* **genus** 

**1. Introduction** 

Yu-Tang Tung, Meng-Thong Chua, Sheng-Yang Wang, Shang-Tzen Chang, 2008. Antiinammation activities of essential oil and its constituents from indigenous *Cinnamon* (*Cinnamomum osmophloeum*) twig. Bioresource Technology 99, 3908- 3913
