**4. Conclusion**

196 Chromatography and Its Applications

**Carbon (C) (H) δ Literature (CDCl3/CD3OD-5%)1 C H**  1 162.8 - 161.89 - 2 149.7 - 148.91 - 3 115.9 5.71 (dd, 2.0, 2.0) 116.76 5.76 (t, 2.0) 4 31.60 2.40 (m) 31.07 2.41 (ddq, 1.6,

5 45.70 1.82 (d, 2.0) 45.26 1.86 (ddd, 1.6,

6a 29.0 2.22 (dd, 2.0, 2.0) 28.80 2.27 (dt, 14.0, 2.0) 6b 1.76 (dd, 2.0, 2.0) 1.73 (dt, 14.0, 2.0) 7 83.6 4.85 (d, 2.0) 83.55 4.76 (d, 2.0) 8 47.5 - 46.74 - 9 39.6 2.52 (d, 2.0) 39.06 2.34 (brd, 4.0) 10 42.7 - 42.06 - 11 75.0 4.59 (d, 6.0) 73.60 4.56 (d, 4.0) 12 77.1 4.32 (d, 6.0) 76.00 4.23 (brs) 13 82.3 - 81.74 -

15 68.5 6.04 (d, 12.0) 67.00 6.09 16 167.5 - 168.24 - 18 170.5 - 171.25 - 19 18.6 1.64 (s) 18.12 1.60 (s) 29 19.9 1.18 (d, 6.0) 19.55 1.20 (d, 6.8) 30a 74.2 4.78 (d, 8.0) 73.6 4.78 (d, 8.0) 30b 3.81 (dd, 8.0, 2.0) 3.77 (d, 8.0) 1' 169.0 - 170.40 - 2' 20.6 1.98 (s) 20.49 2.09 (s) 3' 171.7 - 171.56 - 4' 113.4 6.26 (s) 113.17 6.26 (d, 2.0) OMe (5') 52.9 3.78 (s) 53.02 3,84 (s)

Table 2. Chemical shifts in NMR 1H (200 MHz, CDCl3) and NMR 13C (50 MHz, CDCl3) of

12.0) 49.63 3.24 (brd, 11.0)

14 51.1 3.29 (ddd, 15.0, 2.0, 2.0,

1 Vieira, et al. (2000)

neosergeolide (**2**).

2.0, 6.8)

12.4, 14.0)

The HPLC analysis of infusions (aqueous extracts) of stems and roots of *P. sprucei* revealed higher quantities of isobrucein B than neosergeolide, 40 fold, for both infusions. Considering this information and *in vitro* activity of both compounds, it is very likely that isobrucein B plays more important role for the antimalarial activity than neosergeolide.

Quantification of Antimalarial Quassinoids Neosergeolide and Isobrucein B

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More research is needed to describe seasonal, regional and specimen specific variation in *P. sprucei* quassinoid composition which should have a direct influence on the composition of stem and root infusions prepared from samples of different origins. Knowledge of the extent of these variations, especially as they influence quassinoid composition in infusions, is of fundamental importance given the valuable medicinal and dangerous toxic properties of these widely used Amazonian remedies.

The high performance liquid chromatography has proved to be a powerful tool in the plant extract analysis. The possibility to perform qualitative and quantitative analysis, by HPLC, enables the development of new phytoterapeutical products from the Amazon biodiversity.

#### **5. Acknowledgment**

The autors wish to thank Dr Wanderli Pedro Tadei and CNPq/ FAPEAM - PRONEX, Rede Malária for financial support to the publication of this chapter, Massuo Kato for use of analytical HPLC apparatus and Profs. Norberto P. Lopes and Valquiria P. Jabor for helpful comments regarding this manuscript.

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More research is needed to describe seasonal, regional and specimen specific variation in *P. sprucei* quassinoid composition which should have a direct influence on the composition of stem and root infusions prepared from samples of different origins. Knowledge of the extent of these variations, especially as they influence quassinoid composition in infusions, is of fundamental importance given the valuable medicinal and dangerous toxic properties of

The high performance liquid chromatography has proved to be a powerful tool in the plant extract analysis. The possibility to perform qualitative and quantitative analysis, by HPLC, enables the development of new phytoterapeutical products from the Amazon biodiversity.

The autors wish to thank Dr Wanderli Pedro Tadei and CNPq/ FAPEAM - PRONEX, Rede Malária for financial support to the publication of this chapter, Massuo Kato for use of analytical HPLC apparatus and Profs. Norberto P. Lopes and Valquiria P. Jabor for helpful

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