**2.4 Antimicrobial peptides derived from bovine secretogranin II**

Because bSGII is weakly expressed in the intragranular matrix of chromaffin secretory vesicles (2% of total proteins), the detection of endogenous AMPs by classical methods was unsuccessful. After *in-silico* analysis, two synthetic peptides with cationic amphipathic sequences were prepared: Rrf and Kvk, which correspond to the sequences bSGII131-138 and bSGII430-443 with respective net charges of +4.5 and 4 (Figure 3). Rrf, completely inhibits the bacterial growth of *M. luteus* and *B. megaterium* with a MIC of 5 and 15 µM, respectively, and Kvk displays antifungal properties at 19 µM against *N. crassa* (Shooshtarizadeh et al., 2010).
