**2.6.2 Procedure**

Peeled potatoes (20grams) were cut into small pieces and boiled with 100ml of water for 30 minutes. The pieces are crushed during boiling and the pulp was removed after cooling by filtration through muslin cloth. Dextrose (2grams) and agar (1.5grams) were added and the volume is made up to 100ml. the medium is then distributed in 20ml quantities in two 250ml conical flasks and were sterilized in an autoclave at 121ºC (15lbs/sq. in.) for 30min. the medium was inoculated using 4 days cultures of the test organisms in aseptic condition and transferred to sterile 6 inch diameter petri dishes and allowed to set at room temperature for about 10 minutes. Four cups of 6mm diameter bore in medium at equal distance were made in each agar plate by using sterile borer.

Hexane, chloroform, methanol and water extracts in different concentrations (100mg/ml, 300mg/ml, and 500mg/ml) to get the final drug concentration 5mg/well, 15mg/well, and 25mg/well respectively, control (DMSO) and standard (Bavistin 5μg/ml), were transferred to the cups of each agar plate, incubated at room temperature (28ºC) and examined for inhibition zones of 36 hours of incubation. The results of different studies provide evidence that some medicinal plants might indeed be potential sources of new antibacterial agents even against some antibiotic-resistant strains (Kone *et al.,* 2004).
