**2.7 Antimicrobial activity of peppertree crude methanolic extract and fractions**

For this evaluation an adaptation from the macrodilution method (Clinical and Laboratory Standards Institute 2003*b*) has been used. In a test tube with Müller-Hinton broth already with the extract/fraction in a known concentration, the microorganism to be combated *(Staphylococcus aureus, Pseudomonas aeruginosa or Candida albicans)* was inoculated. To isolate the solvent influence (dimethyl sulfoxide) at the activity of the extract/fraction used, controls having different solvent concentration were prepared. The test tubes were incubated and after that the turbidity standard was analyzed. For an exact analysis of the results an aliquot of 100μL of each test tube was sowed in a Petri dish with Müller-Hinton agar and incubated at 35ºC for 24 hours for posterior growth analysis by colonies counting. For the yeast the incubation period was 48 hours at 35ºC. The test was carried out in duplicate.
