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**14** 

*China* 

**Genetic Diversity and Population** 

**Differentiation of Main Species of** 

Gao Baojia1,2, Nangong Ziyan2 and Gao Lijie2

*1Hebei North University, Zhangjiakou 2Agricultural University of Hebei, Baoding* 

Longitude

28° 11′N 116°54′E 40° 50′N 114°53′E 31° 53′N 105°16′E 30° 25′N 107°24′E

*Dendrolimus* **(Lepidoptera) in China and** 

**Influence of Environmental Factors on Them** 

As the major forestry pest insects in China, *Dendrolimus* included *Dendrolimus punctatus*  Walker, *D. punctatus tabulaeformis* Tsai *et* liu, *D. punctatus spectabilis* Butler, *D. superans* Butler, *D. houi* Lajonquiere and *D. kikuchii* Matsumura. During sequential outbreaks, economical damage can be so extensive for forest appears to be burned and unable to withstand such a long period of defoliation. Gene diversity and genetic structure among the main species of *Dendrolimus* were assessed using morphological diversities, allozyme, Random Amplified Polymorphism DNA(RAPD), Amplified Fragment Length Polymorphism (AFLP), Inter-Simple Sequence Repeat (ISSR), mitochondrial DNA and Simple Sequence Repeat (SSR), which provide the powerful tool for investigation of genetic variation. The influence of ecological factors on the genetic diversity is also discussed by the

**2. Genetic diversities among the main species and geographical populations** 

For investigating the morphological diversity, many pupae of *D. punctatus* Walker, *D. superans* Butler, *D. houi* Lajonquiere and *D. kikuchii* Matsumura were collected in

Species Location Code Latitude and

YJM ZJKL GYYN LPS

**2.1 Morphological diversities among 4 species of** *Dendrolimus* 

Table 1. Origin of 4 different species of the tested *Dendrolimus* materials

*D.punctatus* Walker: Yujiang , Jiangxi *D.superans* Butler: Zhangjiakou, Hebei *D.houi* Lajonquiere: Guangyuan, Sichuan *D.kikuchii* Matsumura: Liangping, Chongqing

**1. Introduction** 

correlation analysis.

**of** *Dendrolimus*

2006(table1).

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