**6. Spearman rank order correlations R=-0.039 p>0.05**

230 Neuroimaging for Clinicians – Combining Research and Practice

**53,63 52,85 57,75**

Fig. 12. The immensity of the clinical state (UPDRS), according to the patient's age

Expressed signs

Light signs

Fig. 13. Correlation between the immensity of the clinical state, according to UPDRS and the

vozrast

0 10 20

Minimal signs

30 40

50 60

Mean (adge)

age of the respondents with IPD

UPDRS

signs

**45**

Extremely expressed

In our research, we've concluded that direct or positive statistic significant correlation between the number of hospitalizations and the immensity of the clinical state, according to UPDRS, indeed exists.

The value of the Spermen's coefficient (r=0.48), shows that these two parameters are proportionally connected, in other words, patients with IPD difficult manifestations of the disease, according to UPDRS scale, have more hospitalizations in the University clinic of Neurology in Skopje.

Table 14 and picture 14, show the results from the crostabulation between the CT finding and MRI of brain and the immensity of the clinical state, determined according UPDRS scale. In the respondents group with light disease symptoms, according to UPDRS scale, dominant are the findings with normal level, previously determined with CT and MRI of brain, present in 5 patients (45.4%), from this group. Between the respondents with light disease symptoms, the finding from CT and MRI of the brain shows discrete cortical reduced changes in 6 patients (46.1%). In half of the respondents, the finding from CT and MRI of brain, shows presence of discrete cortical reduced changes.75% of the respondents with highly expressed disease symptoms, have global cortical reduced changes, proved on CT and MRI of brain.


Table 14. Immensity of the clinical state (UPDRS) in relation to CT and MRI of brain

The connection or the correlation between the immensity of the clinical state, determined by UPDRS scale and brain CT/MRI finding, is also proved with the usage of Spearsmen's correlation coefficient. Its value (r=0.2) shows presence of direct, positive correlation between these two parameters, in other words, there is a match between the brain CT/MRI finding and the results from the UPDRS scale. In patients with difficult clinical forms of IPD, most often the brain CT/MRI finding shows global cortical reduced changes. But, the confirmed correlation is statistically insignificant, (p>0.05), picture 15.

Clinical and Genetic Aspects in Patients with Idiopathic Parkinson Disease 233

After amplification of a region from egzon 3 of SNCA gene, with usage of the following primers: 5'GTC TCA CAC TTT GGA GGG TTT C-3 and 5'-CAC CTA CCT ACA CAT ACC TCT GAC TC-3', the result was an amplified product with length of 395 base pairs (bp). PCR amplification of a region from egzon 4 of SNCA gene, was performed with these primers 5'- GTC AAT CAG CAA TTT AAG GCT AG-3' and 5'-GAT ATG TTC TTA GAT GCT CAG-3', and the amplifications have length of 215 bp. The amplified products were digested with proper restrictive endonuclease under optimal conditions (puferian solutions suitable for each enzyme separately on temperature of 37 c). With that, no mutated sequence in the PCR

G88C mutation in egzon 3 of SNCA gene isn't detected in our 32 respondents with IPD. No

Fig. 16. Electroforetogram from PCR-RELP analysis (agaros gels colored with etidium

Fig. 17. Electroforetogram from PCR-RELP analysis (agaros gels colored with etidium

bromide and photographed on ultraviolet light.

bromide and photographed on ultraviolet light

mutations were found in the G209A in the enzon 4 from SNCA gene, also.

**7. Mutation detection** 

product is found.

Fig. 15. Correlation between the immensity of the clinical state (UPDRS) and brain CT/MRI finding Spearman Rank Order Correlations R=0, 2 p>0.05
