**5. Conclusion**

32 Advances in Unconventional Lithography

(a)chemical synapse structure x40000 bar=0.5μm (b)electrical synapse structure x60000 bar=0.2μm Fig. 18. Under TEM, synapse like structure between neurons from the striatum and the

(a) LN group, width of lines 150μm, (b) PLL group, width of lines 150μm (c) PEI group, width of lines

Fig. 19. Immunofluorescent image of anti-GABA (green fluorescence) +anti-MAP2 (red fluorescence) labelled striatal neurons cultured for 7 days on different substrates by

200μm , (d) LN+PEI group, width of lines 300μm bar=200μm

microfluidic technique x100

substantial nigra on PEI patterns

In summary, this study based on previous work improve the microfluidic technique, evaluate the influence of two different soft lithography, the micro-contact printing and microfluidic technique on various interface materials for the construction of neural network. Our future work can be divided into two levels. On the one hand continue to look for the intersection of microelectrode array and the micro-fabrication technology, trying to make cell grow in accordance with patterns of MEA electrode nodes. On the other hand, need to further improve the characteristics of electrode materials, enhance biocompatibility under the premise of improving signal-to-noise ratio and without adding resistance, do good to the survival of neurons and neurite extension.

The application of the micro-fabrication on microelectrode array may open up a broader platform of the technique for neurochip research and provide new ideas for the treatment of various injuries in the central nervous system. Ultimately, the combination both can achieve position fixing between neurons and electrodes precisely, to make the system as a real sensor, be able to accept electrical stimulation or chemical stimulation and record their signals, to analysis the transfer process of neural network information, and apply to drug screening of related diseases.
