**2.2.3 Troubleshooting**

Although microdialysis is generally a robust technique if difficulties do arise and dialysate is not obtained, a systematic approach is advocated. The micropump should be examined and new batteries placed (if not already done). The catheter insertion site should be examined to check that the catheter remains secure and is not obviously displaced. The microvials can be removed and replaced to ensure that they click into place appropriately. Once confident these components are satisfactory the system may be flushed by re-opening and then closing the lid of the micropump.
