**4. Conclusion**

This work focuses on optimizing the performance of integrated flow confinement microfluidic biosensors for the immunoassay of SARS-CoV-2. Detection is based on the efficiency of the reaction kinetics of SARS-CoV-2 under the effect of certain parameters such as the inlet rate of the flow confinement and the inlet concentration of the analyte. The confining flow improved the transport of the analyte to the reaction surface of the biosensor and significantly reduced the mass consumption of the sample. Increasing the flow velocity by flow confinement can effectively decrease the thickness of the diffusion boundary layer and therefore increase the association and dissociation rates of the virus in question. This study helps to improve future designs of microfluidic biosensors that can be used for rapid virus detection.

*New Advances in Biosensing*
