**4.1.3 Transfection**

	- a. Add 0.5 g of UV-damaged or undamaged (serve as a control) pCMV-Luc together with 0.05 g of internal control plasmid (pRL-CMV, Promega) in 50 μl of Opti-MEM (Invitrogen) medium, mix gently.
	- b. Mix 1 l LipofectamineTM 2000 gently in 50 μl of Opti-MEM medium and incubate for 5 minutes at room temperature.
	- c. Combine the diluted DNA with the diluted LipofectamineTM 2000 (total volume is 100 μl). Mix gently and incubate for 20 min at room temperature to allow the formation of DNA-LipofectamineTM 2000 complexes.
