**4.1 HCR for NER**

NER is responsible for the repair of bulky DNA lesions induced by UV and a lot of anticancer drugs. Here we use UV as a method to damage a luciferase reporter plasmid. Other chemicals (such as cisplatin) that cause bulky DNA adducts can also be used.

### **4.1.1 Materials**

1. The reporter plasmid: pCMV-Luc (Liu et al., 2004). The firefly luciferase is driven by the cytomegalovirus (CMV) immediate early (IE) gene promoter.

