Fig. 1. p130Cas and p140Cap structure.

A) p130Cas consists of an N-terminal SH3 domain, a substrate domain (SD), a serine rich region (SRR), and a C-terminal domain (CT). The main interactors are indicated. In particular, many proteins associate to the N-terminal domain and the Src family kinases (SFKs) bind the CT domain. The 15 YxxP motifs are phosphorylated by Src family kinases to mediate Crk binding.

B) p140Cap consists of an N-terminal tyrosine–rich region (Tyr-rich), an actin binding domain (ABD), a proline rich domain (Pro1), a coil-coiled region (C1-C2), two domains rich in charged amino acids (CH1, CH2) and a C-terminal proline rich domain (Pro2). Src, p130Cas, EB3 and Vinexin bind to the Pro2 domain of p140Cap. The binding regions of Cortactin and Csk have yet to be defined.
