**2.4 Diagnostic laboratory testing**

#### **2.4.1 Detection of antigen**

RABV antigens were detected using the direct fluorescent antibody (DFA) test of skin biopsy specimens, touch impressions of corneal epithelial cells, or fresh brain tissue as described (CDC, 2006). Skin biopsy specimens were taken from the nuchal area of the neck where viral antigens can be present in hair follicles containing cutaneous nerves, as described previously (Noah, 1998).
