**3. Experimental animals**

Healthy adult Swiss albino mice of both sexes (20 to 35 g and 6 to 8 weeks old) are used for testing. All mice are fed commercial pellets and must have access to water ad libitum, alternately. Mice must be acclimated one week before experience in all procedures to minimise stress. All mice used in studies should be handled according to internationally accepted standard guidelines for the use of laboratory animals [39, 40].
