**3. Research methods and analysis of metabolic alertness during salt stress**

Metabolic alertness during salt stress can be studied using a range of research methods and analyses. These can include *in vitro* studies, animal studies, and human studies. *In vitro* studies involve culturing cells and introducing salt stress to observe the metabolic alertness response. This can be done using a range of techniques such as fluorescence microscopy, flow cytometry, and metabolite assays. Animal studies involve exposing animals to the plants grown under salt stress and observing their metabolic alertness response. This can be done using behavioral tests, metabolic assays, and gene expression analyses. Human studies involve exposing individuals to salt stress and observing their metabolic alertness response. This can be done using various methods such as dietary interventions, metabolic assessments, and physical activity tests. The analysis of metabolic alertness during salt stress will depend on the research method used. For *in vitro* studies, the data can be analyzed using a variety of techniques such as statistical tests, machine learning algorithms, and network analyses. For animal studies, the data can be analyzed using techniques such as behavioral tests, metabolomic profiling, and gene expression analyses. For human studies, the data can be analyzed using techniques such as dietary interventions, metabolic assessments, and physical activity tests [55]. In case of salt stress in plants [56, 57], metabolic alertness during salt stress in plants can be studied by a variety of methods and techniques have been mentioned below:
