**1. Introduction**

Chlamydiae are nonmotile, gram-negative, cocci [1]. They are obligate intracellular microorganisms with a biphasic life cycle. They can only grow in live cell cultures [2]. Chlamydias belong to the Chlamydiaceae family, from the order Chlamydiales. Species that cause disease in humans are *Chlamydia trachomatis*, *Chlamydophila psittaci,* and *Clamydophila pneumoniae* [3, 4]. Among them, *C. trachomatis* is among the most common sexually transmitted diseases (STIs) in the world today [5]. According to the World Health Organization (WHO) data, around 90 million new cases of chlamydial infections occur worldwide every year [6]. *Chlamydia trachomatis* has been included in the group D notifiable diseases as a sexually transmitted disease agent in our country since 2005 [7].

There are many diagnostic tests used in the diagnosis of sexually transmitted diseases. Laboratory tests are critical because most of the patients are asymptomatic [8]. Developing countries need cost-effective, fast, reliable, sensitive, and specific laboratory services. The high sensitivity of the tests is important in preventing transmission and complications, directing treatment, and controlling infection [9]. Chlamydia testing is indicated for patients with STIs who have urogenital, anorectal, and ocular symptoms, people who have had sexual contact with patients with STIs, and people who are screened for chlamydia [10].
