Meet the editors

Oscar Núñez studied chemistry at the University of Barcelona, where he also obtained his Ph.D. in 2004. Since June 2020 he has been a professor in the Analytical Chemistry Department of the same university. He has more than 150 scientific papers and book chapters to his name, and he is the editor of seven books on liquid chromatography, LC-MS/MS, sample preparation techniques in food analysis, capillary electrophoresis, and

food integrity and authenticity. He has extensive experience in the development of liquid chromatography methods with ultraviolet and fluorescence detection, liquid chromatography coupled to low- and high-resolution mass spectrometry, as well as in sample treatment procedures for environmental and food analysis. Currently, his main research interests involve the characterization, classification, and authentication of food and natural products, as well as the prevention of food fraud.

Sònia Sentellas has been a Serra Húnter lecturer in the Department of Chemical Engineering and Analytical Chemistry of the University of Barcelona since 2020. She had previously worked as a researcher in the pharmaceutical company Almirall, S.A. for nearly 20 years, focusing on the study of the metabolism of drugs and bioanalysis. Her research interests cover food analysis, including characterization, classification, and authentica-

tion of food products, fraud prevention, and the recovery of bioactive compounds from agrifood waste. She is a member of the Research Institute for Food Nutrition and Food Safety.

Mercè Granados, Ph.D., is an Associate Professor in the Department of Chemical Engineering and Analytical Chemistry, at the University of Barcelona. She has supervised master's and Ph.D. students and has published more than 80 scientific papers. Her wide research interests include topics related to environmental analytical chemistry and the fate of contaminants, as well as the analysis of residues in food, with liquid chromatography and

mass spectrometry being the main techniques applied in these studies. Currently, she is involved in circular-economy projects related to the recovery of bioactive substances from agrifood waste.

Javier Saurina is a full professor in the Department of Chemical Engineering and Analytical Chemistry at the University of Barcelona. A graduate in chemistry (1988) and pharmacy (1996), and with a Ph.D. in chemistry (1997), his current research focuses on the development of new analytical methods, the characterization and authentication of food products, and data processing. He has published more than 20 book chapters

and 190 articles in indexed journals in the fields of analytics, pharmaceuticals, materials science, and food. He is among the 2% most cited and influential authors according to the updated science-wide author databases of standardized citation indicators published in August 2021 (see https://elsevier.digitalcommonsdata.com/ datasets/btchxktzyw/3).

## Contents



Preface

Increasing interest in topics related to health and quality of life in recent years has led to a growing need in food, environmental and bioanalytical research for high-throughput separation techniques able to cope with the qualitative/quantitative determination of a large number of compounds in very complex matrices. High-performance liquid chromatography (HPLC) is a well-established separation technique widely employed in many fields. The versatility of chromatographic separation modes, coupled with low-resolution and high-resolution mass spectrometry, makes HPLC among the best

This book provides an overview of new advances in high-performance liquid chromatography and its applications in different fields. The first of the book's two sections is devoted to principles and new advances in HPLC. In Chapter 1, "Principles and Applications of Ultra-High-Performance Liquid Chromatography", Ahmed et al. describe the fundamentals of UHPLC techniques (solvent delivery systems, columns, sample injection, and detection), and review the role of UHPLC in different fields such as the analysis of herbal medicines, drugs in human plasma, pharmacokinetics and bioavailability studies, identification of metabolites (metabolomics), detection of impurities, analysis of dosage formulations, food safety and agricultural applications.

The higher separation capacity of 2D-LC techniques can be especially useful. A commercial application, the TurboFlow technology developed by Thermo Fisher Scientific, may enable the direct introduction of biological samples into an online automated extraction system without any pre-treatment. In Chapter 2, "Turbulent Flow Chromatography: A Unique Two-Dimensional Liquid Chromatography", Di Gaudio et al. explain the principles of turbulent flow chromatography, the different column chemistries available, the hardware employed, how to operate in multichannel systems, and how to develop a focus mode method to implement this technique. They also describe successful applications of the turbulent flow technique in different fields, including therapeutic drug monitoring and environmental analysis, and applied to

different matrices such as urine, plasma, food commodities and water.

Monolithic media are excellent substitutes for conventional particle-packed columns. This is because monolithic columns show higher permeability and lower flow resistance than conventional liquid chromatography columns, providing high-throughput performance, resolution and separation in short run times. In Chapter 3, "Monoliths Media: Stationary Phases and Nanoparticles", Hernawy et al. address the general characteristics and properties of monolithic materials, the separation mechanism involved, and the different types of monoliths: organic polymer, silica and metal. Applications of monolithic materials in LC separations and nanoparticle-based monoliths are also described.

In Chapter 4, "Perspective Chapter: High-performance Liquid Chromatography Coupled to Mass Spectrometry – The Advance in Chemical Analysis", da Silva Bezerra presents the advantages and limitations of LC-MS techniques, addressing the principles

options to solve emerging analytical problems.

*Durga Prasad Mishra and Biswajeet Acharya*
