**7. Practical recommendations**

	- imaging of the changes in the vitreous structures involved in the pathological process;
	- staining of VB cortical layers to control induced PVD intraoperationally;
	- imaging of VB cortical layers adhered to retinal ILM with a possibility to determine the exact sizes of the vitreoschisis in any of the meridians;
	- the possibility to remove the visualized VB layer not only to determine the topographic anatomy but also to prepare histological preparations for light or electronic microscopy or immunohistochemical studies;
	- In cases of the complete removal of VB cortical layers from ILM surface and the presence of small-diameter MH, it is possible to refrain from ILM removal or maculorhexis temporal side only (**Figures 28–32**)

ILM removal in the presence of a middle-sized MH can be done using the classical technique, i.e., circular maculorexis with the catchment of the entire ILM zone including the vitreous cortex adhered to it and the ILM removal with the formation of petals strictly according to the area of the staining agent location keeping the ILM fixation along the edge of the hole and with the inverted flap of any modification; in case of a bilayer vitreous zone in the presence of MH, a layer-by-layer removal with staining of each layer is advisable; in the presence of large-diameter MH, the maximum complete removal of the upper vitreous cortical layer is highly advisable (if possible) followed by staining of underlying tissues and preserving the fixation of this layer to the edges of the MH; In cases of the removal of the ILM with the cortical layer is performed according to the edge of the stained ILM with cortical layers adhered to it. The adhered zone corresponds to the vitreoschisis (**Figures 33–39**).

*Perspective Chapter: The Vitreous Body Visualization Technique in Diagnosis… DOI: http://dx.doi.org/10.5772/intechopen.109264*

#### **Figure 28.**

*Cortical layers contrasting with Vitreocontrast suspension. Posterior vitreous detachment induction.*

**Figure 29.** *ILM contrasting with remaining vitreous body on the surface.*

#### **Figure 30.**

*ILM pilling in the contrasted layer area, temporal side only, laying the lap on the projection of the macular hole. The layer is held in normal position due to Vitreocontrast particles adhesion.*

**Figure 31.** *Vitreal tamponade by the air.*

*Perspective Chapter: The Vitreous Body Visualization Technique in Diagnosis… DOI: http://dx.doi.org/10.5772/intechopen.109264*

#### **Figure 32.**

*OCT image of the patient (7 days after surgery).*

**Figure 33.** *Posterior vitreous detachment induction with Vitreocontrast suspension.*

**Figure 34.** *Posterior vitreous detachment induction with Vitreocontrast suspension.*

**Figure 35.** *ILM flaps forming (a–d).*

*Perspective Chapter: The Vitreous Body Visualization Technique in Diagnosis… DOI: http://dx.doi.org/10.5772/intechopen.109264*

#### **Figure 36.**

*The reposition of inverted ILM flaps in the macular hole projection. ILM flaps held in the normal position due to Vitreocontrast properties.*

#### **Figure 37.**

*Cortical layer contrasting on the ILM surface. Cortical layer tightly bound to ILM had clear boundaries (blue arrows) and occupies a certain area that can be measured (black arrows).*

**Figure 38.** *ILM flaps formation.*

#### **Figure 39.**

*Macular hole blocked by the inverted flap. ILM removed in the contrasted zone area, in this case ILM removed area corresponded with vitreoschisis zone after abnormal posterior vitreous detachment progress.*

*Perspective Chapter: The Vitreous Body Visualization Technique in Diagnosis… DOI: http://dx.doi.org/10.5772/intechopen.109264*
