**9. Dye injection techniques**

There are different ways to protect the RPE cells during dye injection in MH surgery:


The most common techniques in dye injection are:

1.The "dry method" consists of removing the balanced salt solution (BSS) in the vitreous cavity by a fluid-gas exchange before dye injection. The technique has the advantage of concentrating the dye in the posterior pole, exposing the retinal surface to a higher concentration of dye in the vitreoretinal interface

2.The "wet method" while the surgeon injects the dye in a fluid-filled vitreous cavity. The amount of dye in contact with the retinal surface is lower because it is diluted by the fluid in the vitreous cavity. The wet method is safer and faster but may be less effective in ILM staining, particularly in highly myopic eyes. If a poor stain is encountered, it is often worth re-staining as there may be greater stain uptake on the second attempt.
