*5.2.6 Enzyme-linked immunosorbent assay (ELISA)*

When other tests are negative and there is significant clinical doubt, ELISA is the test of choice for focused, difficult, and chronic patients. This test has high sensitivity and specificity. Moreover, it may detect immunoglobulins (IgG, IgM, and IgA) during 4 to 6 hours. According to the research done in this area, ELISA is a highly effective tool for both the detection of *Brucella* antibodies and the discrimination between the

acute and chronic stages of the disease in large populations. It has been proven by the research community that ELISA assays are quick, accurate, sensitive, and specific. This technique complements agglutination assay in clinical laboratories for the detection of brucellosis and provides an alternative for large-scale screening.
