**5.3 Molecular assays**

The traditional methods of *Brucella* typing are gradually being replaced by newer molecular technologies. The laboratory diagnosis of human brucellosis has benefited from advancements in molecular-based technology. Pure *Brucella* culture DNA and DNA from clinical specimens can both be amplified and detected using PCR tests. Extracting *Brucella* DNA from whole blood, serum, and tissue samples is now possible with the help of a wide variety of commercial kits.
