**6. Detection of** *Brucella* **antibodies in livestock milk**

The milk ring test (MRT) was first introduced by Fleischhauer in Germany in 1937; it is the best test for screening the milk of suspected cases of animal brucellosis. MRT is characterized by simplicity, ease, accuracy, and inexpensive method, also MRT is not consumed time. Therefore, MRT is considered the method of choice for the surveillance of dairy herds. This test mainly detects the *Brucella* antibodies IgM and IgA in fresh milk. The sensitivity and specificity of MRT are 85% and 95%, respectively [39–41].
