*5.2.1 Rose Bengal test (RBT)*

Although this test was developed for veterinary screening, it is now routinely used to diagnose human brucellosis. The Rose Bengal test is a rapid (5–10 minute), simple to conduct, and highly sensitive diagnostic tool for acute brucellosis; nevertheless, it has a high rate of false-negative results in chronic and severe cases, and false-positive results emerge due to cross-reaction with *Yersinia enterocolitica*.
