**6. Adherence of** *Candida albicans* **on PMMA in probiotics solution**

It has been strongly counseled that probiotic consumption improves oral healthiness. However, the impact of probiotics at the microbial fame of denture wearers remains blurring. The acrylic prosthesis dentures in edentulous people possess a non-dropping, tough floor thereby facilitating candida adhesion and subsequent fungal colonization. The ability of the yeast to stick to the epithelial cells superficially and to the fitting denture floor remains as the essential key requirement for colonization of candida species and outcomes in making the denture as a store of contamination.

To evaluate the adherence of *Candida albicans* on PMMA denture base materials, many *in vitro* studies were conducted. In one study, two groups of PMMA dentures were experimented. First group of PMMA dentures was coated with probiotic solution at varying concentrations dipped in saliva containing candida species, and the other PMMA denture group was dipped in saliva containing candida species without probiotics. The result of this study is shown in **Figures 3** and **4**, and **Tables 1** and **2**.

This study had two important result:


*Adherence of* Candida albicans *on Polymethyl Methacrylate in Probiotics Solution DOI: http://dx.doi.org/10.5772/intechopen.112321*

### **Figure 3.**

*The detection rate of candid species was 92.0% in the control group and it was reduced to 16.2% in the test group [12].*

### **Figure 4.**

*Depicting the comparison of probiotics in varying concentrations against candida species [12].*


### **Table 1.**

*Depicting the comparison of probiotics in varying concentrations against candida species [12].*

One other study showed that adhesion and colonization of probiotic bacteria on PMMA dentures prevent attachment of *Candida albicans* through competition for adhesion sites, nutrients, and products of environmental change. In this study, five groups containing 20 PMMA dentures were examined. Each of these groups was


#### **Table 2.**

*Depicting the comparison of* Candida albicans *colony count in varying probiotic concentrations [12].*

dipped in Candida species and then exposed to different substance including the following: (1) probiotic solution of *L. rhamnosus GG*, (2) probiotic solution of k12 *Streptococcus salivarius*, (3) sodium hypochlorite solution, (4) normal saline, and (5) nystatin solution. According to the results of this study, probiotic solutions can be used to reduce the number of *Candida albicans* adhering to the PMMA denture base [13].
