**Electrophoresis for proteins**

1.Lyophilization of samples

The samples were lyophilized by the freeze-dryer lyophilization technique, where the samples to be lyophilized were placed in plastic containers and then placed in a lyophilization device at a temperature of 26°C until almost most of the water was removed, after which powdered was used in protein electrophoresis on polyacrylamide gel in the presence of sodium dodecyle sulfate (SDS) by SDS-PAGE method according to [22].

	- A. Sample preparation:

2g of lyophilized samples were crushed with 14 ml of cooled acetone three times, then the powder was thoroughly mixed with the extraction solution consisting of 0.2M sodium phosphate, 5% SDS, and 4 molar urea pH 7.0. The extraction solution was prepared by dissolving 3.12g sodium phosphate dissolving NaH2PO4, 5 g SDS, and 24.024 g urea in a volume of distilled water, the pH was adjusted to 7.0 and the volume was filled to 100 ml with distilled water, and then centrifuged at 4000 cycles min-1 for 15 min. The protein was precipitated using acetone in a ratio of 1:4 (volume:volume) and centrifuged at a speed of 10,000 cycles min-<sup>1</sup> , the filter was neglected, and the precipitate was taken and dissolved in the buffer solution of the sample.

B. Electrophoresis: Protein electrophoresis was carried out on a Polyacrylamide gel using the Slab–electrophoresis method in the presence of SDS according to the method of [23] and described by [24].

### 3.The solutions used:


*The Role of Some Pre and Postharvest Applications on Storage Behavior and Protein Pattern… DOI: http://dx.doi.org/10.5772/intechopen.109899*

