**3. Materials & methods**

Medicinal plant samples for the study were collected from different areas of Maharashtra with the help of experts and subjected to authentication at by a botanist. All the samples were washed under running tap water and also rinsed with deionized water to remove any earthy matter before drying in an oven at 60°C for near about 2–3 days. Milling of the plant materials was done in a grinder (**Table 1**). Experiments were performed with dried samples of medicinal plants No. 1 – No. 4, (No. 1 – *Azardirachta indica,* No. 2 – *Vitex nigundo*, No. 3 – *A. mexicana*, No. 4 – *Trigonella fornum graecum*,)


#### **Table 1.** *Selected plants information.*
