**Acknowledgements**

I would like to acknowledge the Central University of Technology-Research Office UCDP Grant-for funding the publication.

The CUT Faculty of Health and Environmental Sciences assistance Dean's office: Prof T Makhafola for financial assistance.

The Family, colleagues, and Friends for encouragement.

Thanks to Dr. LF Mogongoa, SD Mokgawa, DE Rampana-Moleleki, D Mathiase, Dr. I Manduna, B Nakedi, A Molelekoa and M Motaung for proof-reading the manuscript and provide constructive comments.
