**3. Miscellaneous**

2-Benzoylpyridine N(4)-cyclohexyl thiosemicarbazone-Anti-proliferative activity of the ligand and its indium complexes was tested against human hepatocellular carcinoma. The studies revealed that the indium complex has better activity than the others [78]. N4-(2-Hydroxy-5-chlorobenzyliden e)-2-amino-5-chlorobenzophenone thiosemicarbazone; N4-(2-Hydroxy naphthalene-1-carbaldehyde)-2-amino-5-chlorobenzophenone TSC and its complexes of Ru(II) cytotoxic activity was studied and reported that, all the complexes showed better in vitro cytotoxic activity against MCF-7, Hop62, MDA-MB cell lines [79]. 7-Chloroquinoline thiosemicarbazone and its complexes of Ga(III), Cytotoxic and antimalarial activity were tested and proved that the complex shows 31 times better activity on colon cancer cell line than etoposide. The complex has better antimalarial activity against *Plasmodium falciparum* than lumefantrine [80].

2-Acetylpyridine-N(4)-Orthochlorophenyl thiosemicarbazone and their complexes of Ga(III), Sn(IV), Pd(II) and Pt(II) Cytotoxic activity was reported. The results indicate the ligands and metal complexes showed better cytotoxic activity [81]. (Z)-(2-((1,3-Diphenyl-1H-pyrazol-4-yl) methylene) and hydrazinyl) (pyridin-2-ylamino) methane-thiol thiosemicarbazones and their complexes of Mn(II), Co(II), Ni(II), Cu(II), Zn(II), Cd(II) Cd(II) and Zn(II) complexes had strong antioxidative property. Ni(II) complex shows excellent activity against HepG2 and MCF-7 cancer cell line [82]. Cd(II) and its complexes of 2-Acetylyuridine-N4 substituted thiosemicarbazones and their Cytotoxic activity against human breast cancer cell lines was tested and found that one of cadmium complexes has better activity than the cisplatin drug [83]. Bi(III) and its complexes of 2,6-Diacetylpyridine bis (4N-methylthiosemicarbazone) have much more anti-bacterial and anticancer activity than its parent ligand against *Bacillus cereus* and *Salmonella Typhimurium* and K562 leukemia cells [84]. Ru(II) and its complexes of Benzaldehyde thiosemicarbazones and their Cytotoxic activity were evaluated against the WHCO1 esophageal cancer cell line. Among the tested complexes two had moderate activity against the cancer cell line [85]. 2-Acetylpyridine thiosemicarbazone; 2-Acetylpyridine N(4)-methyl TSC; 2-Benzoylpyridine TSC; 2-Benzoyl pyridine N(4)-methyl TSC; 2-Benzoylpyridine N(4)-phenyl TSC and its complexes of Mn(II), Co(II), Zn(II) was tested against its cytotoxicity and reported that compounds show significant activity against K562 leucocythemia cancel cell line [86].

2-Pyridineformamide thiosemicarbazones, 2-formyl and 2-acetyl acetyl pyridine thiosemicarbazones and its complexes of Ga(III) antiproliferative activity were evaluated against human cancer cell lines (MCF-7, T24, A549 and mouse L-929). 2-acetyl pyridine thiosemicarbazone shows higher activity than others against all cancer cell lines [87]. Kowol et al. [72] studied the anticancer activity of 2-Acetylpyridine N, N-dimethyl TSC, 2-acetyl pyridine N-pyrrolidinyl TSC, acetyl pyrazine N, N-dimethyl TSC, acetyl pyrazine N-pyrrolidinyl and acetyl pyrazine-N-piperidinyl TSC and its complexes of Ga(III), Fe(III). Cytotoxic activity of the ligands was enhanced by the chelation with Ga(III) while weakening with Fe(III). Noblia et al. [88] reported cytotoxic activity of 5-Bromo salicylaldehyde semicarbazone; 2-Hydroxy-naphtalen-1-carboxaldehyde semicarbazone and their

vanadium complexes. The results indicate that the complexes had selective activity against TK-10 cell line. Anticancer activity of ortho-Naphthaquinone thiosemicarbazone and its Cu(II),Ni(II), Pd(II) and Pt(II) complexes were tested against MCF7 human breast cancer cell lines and results revealed that chelation of metal ion into ligand was enhanced its activity [89]. Casas et al. reported the cytotoxic activity of Formylferrocene thiosemicarbazones and their Au(III) complexes were evaluated against HeLa cell lines and found that antiproliferative activity was similar to cisplatin, a commercial drug.

Arion et al. [90] reported cytotoxic activity of 2-Acetylpyridine 4 N-dimethyl TSC and its Ga(III) complex against human cancer cell lines SW480, SK-BR-3, and 41 M were tested and results revealed that complexes show slightly higher activity than ligand. Antiproliferative activity of Acenaphthenequinone thiosemicarbazone and its Ni(II), Fe(II), Cu(II), Zn(II)were reported and found that Cu(II) complex shows better antiproliferative activity than others. Perez et al. [91] tested the cytotoxic activity of p-Isopropyl benzaldehyde and methyl 2-pyridyl ketone thiosemicarbazones and their Zn(II), Cd(II) against various cell lines. Results indicate that zinc complex was proven as a more potent antitumor agent. Jayasree and Araindakshan [92] reported the antitumor activity of Acetoacetanilide thiosemicarbazone and its Mn(II), Zn(II), Cd(II), Co(II), Fe (IIII) complexes against Ehrlich Ascites tumor cells and found that metal complexes were more active than free ligand. Mohan et al. [93] studied the antitumor activity of 2,6-Diacetylpyridine bis(N4-azacyclic thio semicarbazones) and their Mn(II), Fe(III), Fe(II), Co(II), Ni(II), Cu(II), Zn(II), Pt(II) complexes against P 388 lymphocytic leukemia test system in mice shows that copper complex shows better activity than others.
