**5.4 Comparison of dark duration in light/dark (DDLD) transition box test among the different experimental groups**

The mean ± SEM dark duration for the control, CdCl2 (14 days), CdCl2 (28 days), CdCl2 + Zinc, and CdCl2 + vitamin E groups were 85.00 ± 12.98, 213.50 ± 17.06, 227.66 ± 15.87, 81.63 ± 15.87, and 63.76 ± 18.35 respectively.

The results showed that the CdCl2 group's time spent in the dark chamber was substantially longer (p *<* 0.05) than that of the control group. When compared to the CdCl2 group, the dark chamber duration was considerably shorter (p *<* 0.05) in the CdCl2 + Zinc and CdCl2 + vitamin E groups (see **Table 1**).

*Ameliorative Effects of Zinc and Vitamin E on Physiological Changes after Exposure to Heavy… DOI: http://dx.doi.org/10.5772/intechopen.111518*
