**1. Introduction**

Heavy metals are a special type of toxins that cannot be damaged into non-toxic shapes. The level of these toxic heavy elements has risen dramatically since industrial development [1]. These toxic metals can get into the soil directly *via* the usage of heavy elements and are a special kind of poison that cannot be degraded into nontoxic forms. The concentrations of these toxic heavy metals have advanced dramatically since industrial evolution [1]. These toxic heavy metals can reach into the soil presently by the use of pesticides and fertilizers or indirectly because of wastewater remains, factory emissions, and fossil fuel burning, which might make soils unsuited for cultivation if this trouble aggravates and rises by surpassing certain edges [2]. In

complement to selecting out of the agricultural specialization, soils polluted with heavy elements such as chromium, arsenic, lead, cadmium, copper, zinc, mercury, and nickel assess a significant risk to resources of groundwater through heavy elements filtering. Pollution of harvests cultivated in those soils passively impacts human health and food [3, 4]. The major attraction of environmental contamination investigations is discovering creative methods to rescue the environment from pollutants' damaging impacts [5]. Phytoremediation is a term usually supplied to the mechanisms by which living higher plants can completely attribute to the chemical impacts of the soil they are grown in. In other terms, it is an environmentally suitable technique to collect heavy metals in plant tissues to recycle contaminated soils. The origin of word phytoremediation came from the Greek term "Phyto," which means the plant and the Latin term "remedium," which demonstrates cleaning or rehabilitation [6]. Phytoremediation is a low-cost and practical method for operating soil in evolving countries [7]. The plant species utilized for this purpose are also found in several plant families, such as Asteraceae, Brassicaceae, Fabaceae, Poaceae, Euphorbiaceae, Verbenaceae, and Violaceae [8].

The big-sage (*Lantana camara L*.) plant is an ornamental evergreen shrub grown as a fence plant and the attractiveness of its flowers [9]. The original home of the *L. camara* plant is the subtropical and tropical regions of the American continent and in the tropical areas of Africa and Asia. This species was spread widely almost the world through the eighteenth, nineteenth, and twentieth centuries and evolved into a select evergreen shrub [10]. Further, this shrub earlier revealed favorable findings as shrub phytoremediation [11–14].

*In vitro* culture techniques include being near utilized in phytoremediation investigation [15–20]. Regarding the controlled environment and media technique in these investigations, *in vitro* phytoremediation examinations might provide more precise and dependable findings. Thus, this chapter desired to estimate the effectiveness of the root and vegetative tissues of *L. camara* seedlings in assembling heavy elements (cadmium, cobalt, and lead) via *in vitro* plant tissue culture conditions.
