*13.2.2 Nitric acid and hydrochloric acid*

Alajmi *et al*. [63] stated another method of heavy metal detection in insects by using plasma atomic emission spectrometer [63]. Aphid, leaves and soil samples were oven dried for one hour at 105 degrees, digested for six hours at 550 degrees in a furnace with use of 3 mL of hydrochloric acid then diluted with the deionized water. The targeted heavy metals dried weight was estimated as mg/g and quantified utilizing plasma atomic emission spectrometer.

### **13.3 Plasma optical emission spectroscopy**

Alajmi *et al.* [49] illustrated another method for the quantification of heavy metal concentration in termites and associated soil sample using plasma Optical Emission

Spectroscopy [49]. Samples were oven dried for six to twelve hours at 105 degrees centigrade and measured utilizing microbalance followed by digestion of 0.5 g in 5 mL of nitric acid for six to twelve hours at 105 degree centigrade then added 2 mL of perchloric acid and volume adjusted by deionized water to 10 mL.
