**4.3. Conclusion**

In conclusion, the results of the present experiment have indicated that the data coming from the elevated plus maze and the open field are consistent to each other. However, our results have suggested that elevated plus maze measurements were more sensitive to the melatonin microinjections to amygdala than open field measurements, since the differences were more evident in this maze. This suggests that pinealectomy treatment interacts with anxiety provoking test situations. In open field, it was assumed that the anxiety level experience by animals may be greater in elevated plus maze than open field. In open field the mobility was smaller in pinealectomized rats than controls since the anxiety level may be low compared to the elevated plus maze. This explanation requires further experimental research that illuminates differential effects of pinealectomy on testing conditions.

Taken together, these results are unique contribution to the field of anxiety like behavior and spatial learning in the literature. Further research should take multiple measures of anxiety and learning in the given consideration that melatonin injection produce different outcomes in the investigated parameters in open field, elevated plus maze and Morris water maze.
