**6.1 Role of quorum sensing (QS) mechanism in inhibiting wound healing**

The quorum sensing system is a two-part system made up of an enzyme that catalyzes the synthesis of the signal molecule (auto inducer) and a receptor molecule that binds to the signal molecule (such as acyl-homoserine lactone and cyclic peptides) and controls the transcription of numerous genes in addition to the gene that encodes the signal molecule [54]. Because the bacterial quorum sensing system is required for biofilm development in chronic wounds, it is an important target for anti-biofilm treatment. Because the quorum sensing system is reliant on signaling by auto-inducer chemicals, blocking these would prevent coordinated virulence action. Gram-positive and Gram-negative bacteria have a wide range of quorum sensing systems [55]. The capacity of glyceryl trinitrate (GTN) to suppress quorum sensing-based biofilm development in *P. aeruginosa* burn infections was investigated [56]. The FDA has approved the antibacterial and wound-healing compound glyceryl trinitrate. When administered in ointments at a concentration of 0.15% to 0.3%, it is used to treat anal fissures and to suppress growth tonic *Candida albicans*. *P. aeruginosa* was observed to produce less biofilm when exposed to glyceryl trinitrate [5, 57].
