**2.1 Preparation of hemolysate**

Plasma was isolated from the entire blood sample for the hemolysate sample preparation by centrifugation at 2000 rpm. RBCs were washed three times with normal saline, 5 ml of distilled water was put into a fresh test tube, and 20 μl of packed RBCs were added to the 5 ml distilled water tube, which was gently mixed. Distilled water promotes RBC hemolysis, which results in the creation of hemolysate.
