**Acknowledgements**

All authors had made substantial contributions to the work, with well-established division of tasks. All authors reviewed the final work and approved its submission. All authors agreed to be personally accountable for the author's own contributions and for ensuring that questions related to the accuracy or integrity of any part of the work, even ones in which the author was not personally involved, are appropriately

investigated, resolved, and documented in the literature. All authors have read and agreed to the published version of the manuscript.

This research was funded by Projects PEst-OE/AGR/UI0211/2011 and LA/P/0059/2020 funded by the Portuguese Foundation for Science and Technology (FCT), and COMPETE 2020, from ANI–Projetos ID&T Empresas em Copromoção, by the project "Print-on-Organs–Engineering bioinks and processes for direct printing on organs" with the reference POCI-01-0247-FEDER-033877, by the project "Bone2Move-Development of 'in vivo' experimental techniques and modelling methodologies for the evaluation of 4D scaffolds for bone defect in sheep model: an integrative research approach" with the reference POCI-01-0145-FEDER-031146 and by the PhD scholarships Mariana Vieira Branquinho (SFRH/BD/146172/2019), Ana Catarina Sousa (SFRH/BD/146689/2019), and Bruna Lopes (2021.05265.BD). The author Rui D. Alvites acknowledges Centro de Estudos de Ciência Animal (CECA), Instituto de Ciências, Tecnologias e Agroambiente (ICETA), Porto University (UP), and Fundação para a Ciência e Tecnologia (FCT) for the funding and availability of all technical, structural, and human resources necessary for the development of this work. The author Patrícia Sousa acknowledges Instituto Politécnico de Leiria—Center for Rapid and Sustainable Product Development (CDRSP), University of Porto (UP), Centro de Estudos de Ciência Animal (CECA), Instituto de Ciências, Tecnologias e Agroambiente (ICETA) for the funding (UIDB/04044/2020) and availability of all resources needed for this work. The work was supported through the projects UIDB/04044/2020 and UIDB/00211/2020 funded by FCT/MCTES through national funds.
