**1.2 De novo lipogenesis**

In addition to fat ingested from diet, hepatocytes can produce fatty acids from the oxidation processes of both glucose and amino acids, and synthesize TGs by acetyl CoA. In fact, hepatocytes can also synthesize endogenous cholesterol by the process of De novo lipogenesis (DNL). Cholesterol biosynthesis in the hepatocytes occurs in the endoplasmic reticulum, and involves more than 30 enzymes, such as acetoacetyl CoA, and may contribute to the excess accumulation of fat droplets inside the hepatocytes.
