*1.9.1 Vaginal lavage or smear protocol*

The vaginal smear technique for describing the phases of the estrous cycle was initially probed in 1917 by Stockard and Papanicolaou [26, 27]. A slight adjustment of the method is implemented for this procedure, but it essentially involves the recognition of the cell categories and their relative amounts existing in the sample gotten from flushing walls of the vagina. Ascriptions of the features to explicit phases of the estrous cycle were attributed and recorded by Elvis-Offiah and Bafor [28].
