*Type 1 Diabetes: Current Advances in High-Throughput Technologies and Computational… DOI: http://dx.doi.org/10.5772/intechopen.108248*

low-abundance proteins [56], Standard 2D polyacrylamide gel electrophoresis (2D PAGE) alone has several limitations for biomarker discovery including low throughput and low resolution, and is thus used in conjugation with MS analysis [7]. The two-dimensional liquid chromatography (2DLC) mass spectrometry platform has been widely used in the discovery of protein candidates that may serve as biomarkers [46]. MS and MS combined techniques have been used to identify a number of biomarkers in T1D.
