Meet the editor

Dr. Raffaello Papadakis is a chemical engineer (MEng) with a Ph.D. in Physical Organic Chemistry from the Technical University of Athens, Greece. He has conducted research at various research institutes including CNRS/Aix-Marseille University, France and Ångström Laboratories, and Uppsala University, Sweden. His research interests revolve around physical organic chemistry, materials science, and photochemistry with a partic-

ular focus on the chemistry of graphene and polysaccharides as well as the development of novel fluorophores and bioconjugation. Dr. Papadakis has authored/ co-authored more than forty peer-reviewed scientific papers and book chapters. Currently, he works as a senior research scientist at TdB Labs in Uppsala, Sweden, where he is particularly interested in the fluorescent labeling of polysaccharides.

## Contents


Preface

Fluorescence imaging is a non-invasive technique that is widely known to enable visualization of biological processes occurring within living organisms. It encompasses various methods such as microscopy and spectroscopy and can involve a wide

In bioscience, fluorescence imaging constitutes an indispensable tool, which has over the last years revolutionized the field by empowering monitoring of cellular processes. By using fluorescent dyes or proteins, scientists can label specific molecules or structures within cells and track their behavior in real-time. Consequently, fluorescence imaging has enabled breakthroughs in understanding cellular functions, such as protein interactions, gene expression, and intracellular signaling

The use of fluorescence imaging is quite versatile and is not limited only to biological studies. During the last few years, it has also found applicability in a variety of research and technology areas including medical diagnostics, drug discovery, environmental monitoring, and materials science. For instance, fluorescence imaging can be used to detect cancer cells in tissue samples, monitor the distribution of drugs within the body, or assist in detecting microplastics and their degradation. Additionally, it has been highly contributory in studying the effects of pollutants on living organisms as well as

Acknowledging the quick progress in the field, this book focuses on some new developments within fluorescence imaging. It is organized into three sections. The first section covers novel applications of fluorescence imaging, such as renal intravital microscopy and excitation-emission matrix fluorescence environmental applications. The second section pertains to the development of novel fluorophores and optical chemosensors with an emphasis on graphene-based materials and other optical chemosensors. Finally, the last section deals with the advancement of novel fluorescence imaging techniques and methodologies focusing on time-domain

In my role as the academic editor of this publication, I would like to express my appreciation to all the authors who have contributed chapters encompassing various research and technology domains related to fluorescence imaging. It has been an honor to explore the groundbreaking works in this significant area of research. A big

I would also like to acknowledge TdB Labs AB, the company I have had the privilege to work for as a Senior Research Scientist since 2019. I would especially like to thank

variety of fluorescent imaging probes.

for the characterization of nanomaterials.

fluorescence lifetime imaging.

"thanks" goes to all of you.

pathways.
