**Acknowledgments and funding**

Ana Catarina Sousa (SFRH/BD/146689/2019) and Bruna Lopes (2021. 05265.BD), acknowledge Fundação para a Ciência e Tecnologia (FCT) for financial support. Rui Damásio Alvites acknowledges the Animal Science Studies Centre (CECA), Agroenvironment, Technologies and Sciences Institute (ICETA), Porto University (UP), and FCT for the funding and availability of all technical, structural, and human resources necessary for the development of this work. The author Patrícia Sousa

acknowledges the University of Porto (UP) and Centro de Estudos de Ciência Animal (CECA), Instituto de Ciências, Tecnologias e Agroambiente (ICETA) for the funding (H2Cure: POCI-01-0247-FEDER-047032) and availability of all resources needed for this work. The work was supported through the project UIDB/00211/2020 funded by FCT/MCTES, national funds. This research was funded by Projects PEst-OE/AGR/ UI0211/2011 from FCT and COM-PETE 2020, from ANI–Projetos ID&T Empresas em Copromoção, by the project "Print-on-Organs–Engineering bioinks and processes for direct printing on organs" with the reference PO-CI-01-0247-FEDER-033877, and by the project "Bone2Move- Development of "in vivo" experimental techniques and modeling methodologies for the evaluation of 4D scaffolds for a bone defect in sheep model: an integrative research approach" with the reference POCI-01-0145-FEDER-031146.
