**2.1 Basic methods**

As a practical study, the number of participants in the study was 150 women before and after menopause. A DEXA image was taken for all participants. The participants were divided into three groups according to the current classification of the World Health Organization related to T-Score (normal, osteopenia, and osteoporosis). Normal BMD female participants formed as the control group. Cases of mild osteogenesis imperfecta were diagnosed by a specialist in arthritis and rheumatology according to Sillence standards. The participants were classified into two groups who had symptoms of mild osteogenesis or not. A questionnaire was collected from all the participants, which included information about age, height, weight, body mass index, age of onset of bone complaint, age of the beginning of the menstrual cycle, age of the end of the menstrual cycle, number of children, family history, type of work. Women suffering from hypertension, diabetes, osteomalacia, surgical menopause, and cancer were excluded. Blood samples were collected from the participants on EDTA tubes. PCR, RFLP, DNA sequencing were performed for all samples for two SNPs (LRP5rs121908669 and COL1A2rs72658152). The work was done in accordance with the ethics of scientific research at Tishreen University, Syria.
