**3. Immune-related assays**

There will be an overlap of parameters in these assays. The panels are recombined for different time points. For example, in CAR-T product evaluation and samples were drawn at earlier time points after CAR-T, CAR-expressing cells and other immune subsets may be detected in the same panel. If CAR-T cells continuously decrease and are lower than LOD twice in a row, the MFC method will no longer be used to detect CAR-T cells and the protocol will be changed to immune function and cytokine assays without CAR-T cell detection.
